Freshly prepared immune complexes with intermittent microwave irradiation result in rapid and high-quality immunostaining

Pathology Research and Practice

Volumn 202, Issue 6, 2006, Pages 439-445

  Hatta, Hideki ^a   Tsuneyama, Koichi ^a   Kumada, Tokimasa ^a   Zheng, Huachuan ^a   Cheng, Chunmei ^a   Cui, Zhengguo ^a   Takahashi, Hiroyuki ^a   Nomoto, Kazuhiro ^a   Murai, Yoshihiro ^a   Takano, Yasuo ^a  

^a UNIVERSITY OF TOYAMA   (Japan)

Author keywords

Immune complex; Microwave irradiation; One step immunostaining

Indexed keywords

ANTIBODY;

ACCURACY; ANTIGEN ANTIBODY COMPLEX; ARTICLE; COLON CANCER; CONTROLLED STUDY; DIAGNOSTIC VALUE; FROZEN SECTION; HUMAN; HUMAN TISSUE; IMMUNOCHEMISTRY; IMMUNOHISTOCHEMISTRY; INCUBATION TIME; LYMPH NODE METASTASIS; MICROWAVE IRRADIATION; QUALITY CONTROL;

EID: 33646526730     PISSN: 03440338     EISSN: 16180631     Source Type: Journal    
DOI: 10.1016/j.prp.2006.02.003     Document Type: Article

References (20)

1. Banik S., Bandyopadhyay S., and Ganguly S. Bioeffects of microwave - a brief review. Bioresour. Technol. 87 (2003) 155-159
2. Brown R.W., and Chirala R. Utility of microwave-citrate antigen retrieval in diagnostic immunohistochemistry. Mod. Pathol. 8 (1995) 515-520
3. Cattoretti G., Pileri S., Parravicini C., Becker M.H., Poggi S., Bifulco C., Key G., D'Amato L., Sabattini E., and Feudale E. Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J. Pathol. 171 (1993) 83-98
4. Chilosi M., Lestani M., Pedron S., Montagna L., Benedetti A., Pizzolo G., and Menestrina F. A rapid immunostaining method for frozen sections. Biotech. Histochem. 69 (1994) 235-239
5. Cuevas E.C., Bateman A.C., Wilkins B.S., Johnson P.A., Williams J.H., Lee A.H., Jones D.B., and Wright D.H. Microwave antigen retrieval in immunocytochemistry: a study of 80 antibodies. J. Clin. Pathol. 47 (1994) 448-452
6. Frost A.R., Sparks D., and Grizzle W.E. Methods of antigen recovery vary in their usefulness in unmasking specific antigens in immunohistochemistry. Appl. Immunohistochem. Mol. Morphol. 8 (2000) 236-243
7. Guesdon J.L., Ternynck T., and Avrameas S. The use of avidin-biotin interaction in immunoenzymatic techniques. J. Histochem. Cytochem. 27 (1979) 1131-1139
8. Heitzmann H., and Richards F.M. Use of the avidin-biotin complex for specific staining of biological membranes in electron microscopy. Proc. Natl. Acad. Sci. USA 71 (1974) 3537-3541
9. Hoetelmans R.W., van Slooten H.J., Keijzer R., van de Velde C.J., and van Dierendonck J.H. Comparison of the effects of microwave heating and high pressure cooking for antigen retrieval of human and rat Bc1-2 protein in formaldehyde-fixed paraffin-embedded sections. Biotech. Histochem. 77 (2002) 137-144
10. Hsu S.M., Raine L., and Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29 (1981) 577-580
11. Imam S.A., Young L., Chaiwun B., and Taylor C.R. Comparison of two microwave based antigen-retrieval solutions in unmasking epitopes in formalin-fixed tissue for immunostaining. Anticancer Res. 15 (1995) 1153-1158
12. Jackson P., Lalani E.N., and Boutsen J. Microwave-stimulated immunogold silver staining. Histochem. J. 20 (1988) 353-358
13. Kanai K., Nunoya T., Shibuya K., Nakamura T., and Tajima M. Variations in effectiveness of antigen retrieval pretreatments for diagnostic immunohistochemistry. Res. Vet. Sci. 64 (1998) 57-61
14. Kumada T., Tsuneyama K., Hatta H., Ishizawa S., and Takano Y. Improved 1-h rapid immunostaining method using intermittent microwave irradiation: practicability based on 5 years application in Toyama Medical and Pharmaceutical University Hospital. Mod. Pathol. 17 (2004) 1141-1149
15. Leong A.S. Microwaves in diagnostic immunohistochemistry. Eur. J. Morphol. 34 (1996) 381-383
16. Nomoto S., Nakao A., Ichihara T., Nonami T., Harada A., Ichihara T., and Takagi H. Intraoperative quick immunoperoxidase staining: a useful adjunct to routine pathological diagnosis in pancreatic carcinoma. Hepatogastroenterology 42 (1995) 717-723
17. Taylor C.R., Shi S.R., Chen C., Young L., Yang C., and Cote R.J. Comparative study of antigen retrieval heating methods: microwave, microwave and pressure cooker, autoclave, and steamer. Biotech. Histochem. 71 (1996) 263-270
18. van den Broek L.J., and van de Vijver M.J. Assessment of problems in diagnostic and research immunohistochemistry associated with epitome instability in stored paraffin sections. Appl. Immunohistochem. Mol. Morphol. 8 (2000) 316-321
19. von Wasielewski R., Werner M., Nolte M., Wilkens L., and Georgii A. Effects of antigen retrieval by microwave heating in formalin-fixed tissue sections on a broad panel of antibodies. Histochemistry 102 (1994) 165-172
20. Wester K., Wahlund E., Sundstrom C., Ranefall P., Bengtsson E., Russell P.J., Ow K.T., Malmstrom P.U., and Busch C. Paraffin section storage and immunohistochemistry. Effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen,. Appl. Immunohistochem. Mol. Morphol. 8 (2000) 61-70