Assessment of problems in diagnostic and research immunohistochemistry associated with epitope instability in stored paraffin sections

Applied Immunohistochemistry and Molecular Morphology

Volumn 8, Issue 4, 2000, Pages 316-321

  Van den Broek, L J C M ^a   Van de Vijver, M J ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Epitope instability Antigenicity; Immunohistochemistry; Paraffin sections

Indexed keywords

BLOOD CLOTTING FACTOR 8; CARCINOEMBRYONIC ANTIGEN; CD20 ANTIGEN; CD30 ANTIGEN; CD31 ANTIGEN; CD45 ANTIGEN; CD68 ANTIGEN; CHROMOGRANIN A; COMPLEMENT COMPONENT C3D RECEPTOR; CORTICOTROPIN; CYCLIN D1; DESMIN; EPIDERMAL GROWTH FACTOR RECEPTOR; EPITOPE; ESTROGEN RECEPTOR; FORMALDEHYDE; GROWTH HORMONE; HEPATITIS B CORE ANTIGEN; HEPATITIS B SURFACE ANTIGEN; KERATIN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY KI 67; PARAFFIN; POLYCLONAL ANTIBODY; PROLACTIN; PROSTATE SPECIFIC ANTIGEN; PROTEIN P53; PROTEIN S 100; UVOMORULIN; VIMENTIN;

ADHESION; ANTIGEN RETRIEVAL; ANTIGENICITY; ARTICLE; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; IMMUNOHISTOCHEMISTRY; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE;

ANTIBODIES; ANTIGENS; COLON, SIGMOID; COLONIC NEOPLASMS; EPITOPES; HUMANS; IMMUNOHISTOCHEMISTRY; NEOPLASMS; PARAFFIN; TEMPERATURE; TIME FACTORS;

EID: 0033677607     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: 10.1097/00022744-200012000-00009     Document Type: Article

References (13)

1. Oestrogen receptor staining of paraffinembedded breast carcinomas following short fixation in formalin: A comparison with cytosolic and frozen section receptor analyses
2. Preservation of estrogen recepter in paraffin sections
3. p53 antigen loss in stored paraffin slides
4. Estrogen receptor specific (ER1D5) epitope stability in paraffin sections
5. More on p53 antigen loss in stored paraffin slides
6. Loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer
7. Detectability of retinoblastoma (Rb) gene product and p53 antigen expression decreases in stored paraffin sections
8. Tissue preparation for immunocytochemistry
9. Neu-protein overexpression in breast cancer. Association with comedo-type ductal carcinoma in situ and limited prognostic value in stage II breast cancer
10. Loss of p53-immunostaining intensity in breast cancer
11. Influence of storage temperature and high-temperature antigen retrieval buffers on results of immunohistochemical staining in sections stored for long periods
12. Comparison of p53 immunoreactivity in fresh-cut versus stored slides with and without microwave heating
13. Variability of immunohistochemical reactivity on stored paraffin slides