Comparison of the effects of microwave heating and high pressure cooking for antigen retrieval of human and rat Bcl-2 protein in formaldehyde-fixed, paraffin-embedded sections

Biotechnic and Histochemistry

Volumn 77, Issue 3, 2002, Pages 137-144

  Hoetelmans, R W M ^a   van Slooten, H J ^a   Keijzer, R ^a   van de Velde, C J H ^a   van Dierendonck, J H ^a  

^a Department of Medical Information   (Netherlands)

Author keywords

Antigen retrieval; Bcl 2; Formaldehyde; High pressure cooking; Immunohistochemistry; Microwave heating; Mitotic chromosomes; Nucleus; Western blot

Indexed keywords

BUFFER; CITRIC ACID; CYTOPLASM PROTEIN; EPITOPE; FORMALDEHYDE; MONOCLONAL ANTIBODY; PARAFFIN; PROTEIN BCL 2;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN BINDING; ANTIGEN RETRIEVAL; ARTICLE; CANCER; CELL NUCLEUS; CHROMOSOME; COMPARATIVE STUDY; CONTROLLED STUDY; COOKING; HEATING; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTERPHASE; MALE; MICROWAVE RADIATION; MITOSIS; NONHUMAN; PH; PREDICTION; PROGNOSIS; PROTEIN EXPRESSION; RAT; TEMPERATURE; TISSUE FIXATION; TISSUE SECTION; WESTERN BLOTTING; ANIMAL; HEAT; METHODOLOGY; PRESSURE;

ANIMALIA;

ANIMALS; FORMALDEHYDE; HEAT; HUMANS; IMMUNOHISTOCHEMISTRY; MICROWAVES; PARAFFIN EMBEDDING; PRESSURE; PROTO-ONCOGENE PROTEINS C-BCL-2; RATS; TISSUE FIXATION;

EID: 0036020721     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.1080/bih.77.3.137.144     Document Type: Article

References (30)

1. Nuclear and cytoplasmic bcl-2 expression in endometrial hyperplasia and adenocarcinoma
2. The immunoexpression of bcl-2 and p53 in Kaposi's sarcoma
3. Subcellular localization of the bcl-2 protein in malignant and normal lymphoid cells
4. Comparative study of invasive squamous cell carcinoma and verrucous carcinoma of the oral cavity: Expression of bcl-2, p53, and Her-2/neu, and indexes of cell turnover
5. Microwave-stimulated antigen retrieval is pH and temperature dependent
6. Reaction of formaldehyde with protein. VI. Cross-linking of amino groups with phenol, imidazole, or indole groups
7. Inactivation of Bcl-2 by phosphorylation
8. Bcl-2 and Bax proteins are present in interphase nuclei of mammalian cells
9. Routine formaldehyde fixation irreversibly reduces immunoreactivity of Bcl-2 in the nuclear compartment of breast cancer cells, but not in the cytoplasm
10. High temperature antigen retrieval and loss of nuclear morphology: A comparison of microwave and autoclave techniques
11. Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections
12. Bcl-2 gene family and the regulation of programmed cell death
13. Investigation of the subcellular distribution of the bcl-2 oncoprotein: Residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes
14. Immunohistochemical analysis of Mcl-1 and Bcl-2 proteins in normal and neoplastic lymph nodes
15. Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2
16. Bcl-2 protein localizes to the chromosomes of mitotic nuclei and is correlated with the cell cycle in cultured epithelial cell lines
17. Interferon treatment of a transplantable rat colon adenocarcinoma: Importance of tumor site
18. Brief, high-temperature heat denaturation (pressure cooking): A simple and effective method of antigen retrieval for routinely processed tissues
19. Antigen retrieval techniques in immunohistochemistry: Comparison of different methods
20. Bcl-2 and p53 immunoprofile in Kaposi's sarcoma
21. Mitotic chromosomal bcl-2. I. Stable expression throughout the cell cycle and association with isolated chromosomes
22. Mitotic chromosomal bcl-2. II. Localization to interphase nuclei
23. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections
24. Development of optimal protocols for antigen retrieval immunohistochemistry based on the effects of variation in temperature and pH: Use of a "test battery"
25. Antigen retrieval immunohistochemistry: Past, present, and future
26. Hydrated autoclave pretreatment enhances tau immunoreactivity in formalin-fixed normal and Alzheimer's disease brain tissues
27. Comparative study of antigen retrieval heating methods: Microwave, microwave and pressure cooker, autoclave, and steamer
28. Involvement of the bcl-2 gene in human follicular lymphoma
29. Antigen retrieval for bcl-2 protein in formalin-fixed, paraffin-embedded sections
30. Expression of Bcl-2 in node-negative breast cancer is associated with various prognostic factors, but does not predict response to one course of perioperative chemotherapy