Tissue distribution of UT-A and UT-B mRNA and protein in rat

American Journal of Physiology - Regulatory Integrative and Comparative Physiology

Volumn 290, Issue 5, 2006, Pages

  Doran, John J ^a   Klein, Janet D ^a   Young, Hee Kim ^a   Smith, Tekla D ^a   Kozlowski, Shelley D ^a   Gunn, Robert B ^a   Sands, Jeff M ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Immunohistochemistry; Northern blot analysis; Urea transporter; Western blot analysis

Indexed keywords

CARRIER PROTEIN; ISOPROTEIN; MESSENGER RNA; PROTEIN ANTIBODY; PROTEIN SLA14A1; PROTEIN SLC14A2; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CELL MEMBRANE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; IMMUNOHISTOCHEMISTRY; KIDNEY PARENCHYMA; MALE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; RETICULOCYTE; RNA TRANSCRIPTION; SCREENING TEST; TESTIS; TISSUE DISTRIBUTION; WESTERN BLOTTING;

ANIMALS; BLOTTING, NORTHERN; BLOTTING, WESTERN; BRAIN CHEMISTRY; DNA, COMPLEMENTARY; IMMUNOHISTOCHEMISTRY; KIDNEY; MALE; MEMBRANE TRANSPORT PROTEINS; RATS; RATS, SPRAGUE-DAWLEY; RETICULOCYTES; RNA, MESSENGER; TESTIS; TISSUE DISTRIBUTION;

EID: 33646485107     PISSN: 03636119     EISSN: 15221490     Source Type: Journal    
DOI: 10.1152/ajpregu.00352.2004     Document Type: Article

References (53)

1. Bagnasco SM, Peng T, Janech MG, Karakashian A, and Sands JM. Cloning and characterization of the human urea transporter UT-A1 and mapping of the human Slc14a2 gene. Am J Physiol Renal Physiol 281: F400-F406, 2001.
2. Bagnasco SM, Peng T, Nakayama Y, and Sands JM. Differential expression of individual UT-A urea transporter isoforms in rat kidney. J Am Soc Nephrol 11: 1980-1986, 2000.
3. Berger UV, Tsukaguchi H, and Hediger MA. Distribution of mRNA for the facilitated urea transporter UT3 in the rat nervous system. Anat Embryol (Berl) 197: 405-414, 1998.
4. Bradford AD, Terris JM, Ecelbarger CA, Klein JD, Sands JM, Chou CL, and Knepper MA. 97- and 117-kDa forms of collecting duct urea transporter UT-A1 are due to different states of glycosylation. Am J Physiol Renal Physiol 281: F133-F143, 2001.
5. Couriaud C, Ripoche P, and Rousselet G. Cloning and functional characterization of a rat urea transporter: expression in the brain. Biochim Biophys Acta 1309: 197-199, 1996.
6. Doran JJ, Sands JM, and Timmer RT. Accurate mRNA size determination in Northern analysis using individual lane size markers. Biotechniques 27: 280-282, 1999.
7. Duchesne R, Klein JD, Velotta JB, Doran JJ, Rouillard P, Roberts BR, McDonough AA, and Sands JM. UT-A urea transporter protein in heart: increased abundance during uremia, hypertension, and heart failure. Circ Res 89: 139-145, 2001.
8. Fenton RA, Chou CL, Stewart GS, Smith CP, and Knepper MA. Urinary concentrating defect in mice with selective deletion of phloretinsensitive urea transporters in the renal collecting duct. Proc Natl Acad Sci USA 101: 7469-7474, 2004.
9. Fenton RA, Cooper GJ, Morris ID, and Smith CP. Coordinated expression of UT-A and UT-B urea transporters in rat testis. Am J Physiol Cell Physiol 282: C1492-C1501, 2002.
10. Fenton RA, Cottingham CA, Stewart GS, Howorth A, Hewitt JA, and Smith CP. Structure and characterization of the mouse UT-A gene (Slc14a2). Am J Physiol Renal Physiol 282: F630-F638, 2002.
11. Fenton RA, Hewitt JE, Howorth A, Cottingham CA, and Smith CP. The murine urea transporter genes Slc14a1 and Slc14a2 occur in tandem on chromosome 18. Cytogenet Cell Genet 87: 95-96, 1999.
12. Fenton RA, Howorth A, Cooper GJ, Meccariello R, Morris ID, and Smith CP. Molecular characterization of a novel UT-A urea transporter isoform (UT-A5) in testis. Am J Physiol Cell Physiol 279: C1425-C1431, 2000.
13. Fenton RA, Stewart GS, Carpenter B, Howorth A, Potter EA, Cooper GJ, and Smith CP. Characterization of the mouse urea transporters UT-A1 and UT-A2. Am J Physiol Renal Physiol 283: F817-F825, 2002.
14. Hu MC, Bankir L, Michelet S, Rousselet G, and Trinh-Trang-Tan MM. Massive reduction of urea transporters in remnant kidney and brain of uremic rats. Kidney Int 58: 1202-1210, 2000.
15. Inoue H, Jackson SD, Vikulina T, Klein JD, Tomita K, and Bagnasco SM. Identification and characterization of a Kidd antigen/UT-B urea transporter expressed in human colon. Am J Physiol Cell Physiol 287: C30-C35, 2004.
16. Jung JY, Madsen KM, Han KH, Yang CW, Knepper MA, Sands JM, and Kim J. Expression of urea transporters in potassium-depleted mouse kidney. Am J Physiol Renal Physiol 285: F1210-F1224, 2003.
17. Karakashian A, Timmer RT, Klein JD, Gunn RB, Sands JM, and Bagnasco SM. Cloning and characterization of two new isoforms of the rat kidney urea transporter: UT-A3 and UT-A4. J Am Soc Nephrol 10: 230-237, 1999.
18. Klein JD, Price SR, Bailey JL, Jacobs JD, and Sands JM. Glucocorticoids mediate a decrease in AVP-regulated urea transporter in diabetic rat inner medulla. Am J Physiol Renal Physiol 273: F949-F953, 1997.
19. Klein JD, Sands JM, Qian L, Wang X, and Yang B. Upregulation of urea transporter UT-A2 and water channels AQP2 and AQP3 in transgenic mice lacking urea transporter UT-B. J Am Soc Nephrol 15: 1161-1167, 2004.
20. Klein JD, Timmer RT, Rouillard P, Bailey JL, and Sands JM. UT-A urea transporter protein expressed in liver: upregulation by uremia. J Am Soc Nephrol 10: 2076-2083, 1999.
21. Kwun YS, Yeo SW, Ahn YH, Lim SW, Jung JY, Kim WY, Sands JM, and Kim J. Immunohistochemical localization of urea transporters A and B in the rat cochlea. Hear Res 183: 84-96, 2003.
22. Lim S-W, Han K-H, Jung J-Y, Kim W-Y, Yang C-W, Sands JM, Knepper MA, Madsen KM, and Kim J. Ultrastructural localization of UT-A and UT-B in rat kidneys with different hydration status. Am J Physiol Regul Integr Comp Physiol 290: R479-R492, 2006.
23. Lucien N, Bruneval P, Lasbennes F, Belair MF, Mandet C, Cartron JP, Bailly P, and Trinh-Trang-Tan MM. UT-B1 urea transporter is expressed along the urinary and gastrointestinal tracts of the mouse. Am J Physiol Regul Integr Comp Physiol 288: R1046-R1056, 2005.
24. Lucien N, Sidoux-Walter F, Olives B, Moulds J, Le Pennec PY, Cartron JP, and Bailly P. Characterization of the gene encoding the human Kidd blood group/urea transporter protein. Evidence for splice site mutations in Jknull individuals. J Biol Chem 273: 12973-12980, 1998.
25. Marini JC, Klein JD, Sands JM, and Van Amburgh ME. Effect of nitrogen intake on nitrogen recycling and urea transporter abundance in lambs. J Anim Sci 82: 1157-1164, 2004.
26. Nakayama Y, Naruse M, Karakashian A, Peng T, Sands JM, and Bagnasco SM. Cloning of the rat Slc14a2 gene and genomic organization of the UT-A urea transporter. Biochim Biophys Acta 1518: 19-26, 2001.
27. Naruse M, Klein JD, Ashkar ZM, Jacobs JD, and Sands JM. Glucocorticoids downregulate the vasopressin-regulated urea transporter in rat terminal inner medullary collecting ducts. J Am Soc Nephrol 8: 517-523, 1997.
28. Olives B, Martial S, Mattei MG, Matassi G, Rousselet G, Ripoche P, Cartron JP, and Bailly P. Molecular characterization of a new urea transporter in the human kidney. FEBS Lett 386: 156-160, 1996.
29. Olives B, Mattei MG, Huet M, Neau P, Martial S, Cartron JP, and Bailly P. Kidd blood group and urea transport function of human erythrocytes are carried by the same protein. J Biol Chem 270: 15607-15610, 1995.
30. Olives B, Neau P, Bailly P, Hediger MA, Rousselet G, Cartron JP, and Ripoche P. Cloning and functional expression of a urea transporter from human bone marrow cells. J Biol Chem 269: 31649-31652, 1994.
31. Pawar VP and Prabhu A. Isolation of large numbers of fully viable human reticulocytes using continuous Percoll density gradient. Clin Lab Sci 4: 360-363, 1991.
32. Prichett WP, Patton AJ, Field JA, Brun KA, Emery JG, Tan KB, Rieman DJ, McClung HA, Nadeau DP, Mooney JL, Suva LJ, Gowen M, and Nuttall ME. Identification and cloning of a human urea transporter HUT11, which is downregulated during adipogenesis of explant cultures of human bone. J Cell Biochem 76: 639-650, 2000.
33. Promeneur D, Rousselet G, Bankir L, Bailly P, Cartron JP, Ripoche P, and Trinh-Trang-Tan MM. Evidence for distinct vascular and tubular urea transporters in the rat kidney. J Am Soc Nephrol 7: 852-860, 1996.
34. Shayakul C, Steel A, and Hediger MA. Molecular cloning and characterization of the vasopressin-regulated urea transporter of rat kidney collecting ducts. J Clin Invest 98: 2580-2587, 1996.
35. Shayakul C, Tsukaguchi H, Berger UV, and Hediger MA. Molecular characterization of a novel urea transporter from kidney inner medullary collecting ducts. Am J Physiol Renal Physiol 280: F487-F494, 2001.
36. Sidoux-Walter F, Lucien N, Olives B, Gobin R, Rousselet G, Kamsteeg EJ, Ripoche P, Deen PM, Cartron JP, and Bailly P. At physiological expression levels the Kidd blood group/urea transporter protein is not a water channel. J Biol Chem 274: 30228-30235, 1999.
37. Smith CP, Lee WS, Martial S, Knepper MA, You G, Sands JM, and Hediger MA. Cloning and regulation of expression of the rat kidney urea transporter (rUT2). J Clin Invest 96: 1556-1563, 1995.
38. Smith CP, Potter EA, Fenton RA, and Stewart GS. Characterization of a human colonic cDNA encoding a structurally novel urea transporter, hUT-A6. Am J Physiol Cell Physiol 287: C1087-C1093, 2004.
39. Spector DA, Yang Q, Liu J, and Wade JB. Expression, localization, and regulation of urea transporter B in rat urothelia. Am J Physiol Renal Physiol 287: F102-F108, 2004.
40. Stewart GS, Fenton RA, Thevenod F, and Smith CP. Urea movement across mouse colonic plasma membranes is mediated by UT-A urea transporters. Gastroenterology 126: 765-773, 2004.
41. Stewart GS, Graham C, Cattell S, Smith TPL, Simmons NL, and Smith CP. UT-B is expressed in bovine rumen: potential role in ruminal urea transport. Am J Physiol Regul Integr Comp Physiol 289: R605-R612, 2005.
42. Terris J, Ecelbarger CA, Sands JM, and Knepper MA. Long-term regulation of renal urea transporter protein expression in rat. J Am Soc Nephrol 9: 729-736, 1998.
43. Terris JM, Knepper MA, and Wade JB. UT-A3: localization and characterization of an additional urea transporter isoform in the IMCD. Am J Physiol Renal Physiol 280: F325-F332, 2001.
44. Timmer RT, Klein JD, Bagnasco SM, Doran JJ, Verlander JW, Gunn RB, and Sands JM. Localization of the urea transporter UT-B protein in human and rat erythrocytes and tissues. Am J Physiol Cell Physiol 281: C1318-C1325, 2001.
45. Trinh-Trang-Tan MM, Lasbennes F, Gane P, Roudier N, Ripoche P, Cartron JP, and Bailly P. UT-B1 proteins in rat: tissue distribution and regulation by antidiuretic hormone in kidney. Am J Physiol Renal Physiol 283: F912-F922, 2002.
46. Tsukaguchi H, Shayakul C, Berger UV, Tokui T, Brown D, and Hediger MA. Cloning and characterization of the urea transporter UT3: localization in rat kidney and testis. J Clin Invest 99: 1506-1515, 1997.
47. Uchida S, Sohara E, Rai T, Ikawa M, Okabe M, and Sasaki S. Impaired urea accumulation in the inner medulla of mice lacking the urea transporter UT-A2. Mol Cell Biol 25: 7357-7363, 2005.
48. Wade JB, Lee AJ, Liu J, Ecelbarger CA, Mitchell C, Bradford AD, Terris J, Kim GH, and Knepper MA. UT-A2: a 55-kDa urea transporter in thin descending limb whose abundance is regulated by vasopressin. Am J Physiol Renal Physiol 278: F52-F62, 2000.
49. Wagner L, Klein JD, Sands JM, and Baylis C. Urea transporters are distributed in endothelial cells and mediate inhibition of L-arginine transport. Am J Physiol Renal Physiol 283: F578-F582, 2002.
50. Ward C, Thien F, Secombe J, Gollant S, and Walters EH. Bronchoalveolar lavage fluid urea as a measure of pulmonary permeability in healthy smokers. Eur Respir J 15: 285-290, 2000.
51. Xu Y, Olives B, Bailly P, Fischer E, Ripoche P, Ronco P, Cartron JP, and Rondeau E. Endothelial cells of the kidney vasa recta express the urea transporter HUT11. Kidney Int 51: 138-146, 1997.
52. Yang B, Bankir L, Gillespie A, Epstein CJ, and Verkman AS. Urea-selective concentrating defect in transgenic mice lacking urea transporter UT-B. J Biol Chem 277: 10633-10637, 2002.
53. You G, Smith CP, Kanai Y, Lee WS, Stelzner M, and Hediger MA. Cloning and characterization of the vasopressin-regulated urea transporter. Nature 365: 844-847, 1993.