Impaired urea accumulation in the inner medulla of mice lacking the urea transporter UT-A2

Molecular and Cellular Biology

Volumn 25, Issue 16, 2005, Pages 7357-7363

  Uchida, Shinichi ^a,c   Sohara, Eisei ^a   Rai, Tatemitsu ^a   Ikawa, Masahito ^b   Okabe, Masaru ^b   Sasaki, Sei ^a  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c NONE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; CHLORIDE ION; SODIUM ION; UNCLASSIFIED DRUG; UREA; UREA TRANSPORTER UT A2;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DEHYDRATION; GENE DELETION; HENLE LOOP; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; KIDNEY MEDULLA; KNOCKOUT MOUSE; MOUSE; NEPHRON; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DIET; PROTEIN RESTRICTION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UREA CYCLE; WESTERN BLOTTING; WILD TYPE;

ALLELES; ANIMALS; BIOLOGICAL TRANSPORT; BLOTTING, NORTHERN; BLOTTING, WESTERN; CHLORIDES; EXONS; FEMALE; GENE DELETION; GENOTYPE; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; KIDNEY MEDULLA; MALE; MEMBRANE TRANSPORT PROTEINS; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MICE, TRANSGENIC; MODELS, GENETIC; NEPHRONS; PHENOTYPE; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SODIUM; UREA;

EID: 23344432987     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.25.16.7357-7363.2005     Document Type: Article

References (26)

1. -/- mice kidney. Am. J. Physiol. Renal Physiol. 280:F79-F87.
2. Bagnasco, S. M. 2003. Gene structure of urea transporters. Am. J. Physiol. Renal Physiol. 284:F3-F10.
3. Bagnasco, S. M., T. Peng, M. G. Janech, A. Karakashian, and J. M. Sands. 2001. Cloning and characterization of the human urea transporter UT-A1 and mapping of the human Slc14a2 gene. Am. J. Physiol. Renal Physiol. 281:F400-F406.
4. Bagnasco, S. M., T. Peng, Y. Nakayama, and J. M. Sands. 2000. Differential expression of individual UT-A urea transporter isoforms in rat kidney. J. Am. Soc. Nephrol. 11:1980-1986.
5. Bankir, L., K. Chen, and B. Yang. 2004. Lack of UT-B in vasa recta and red blood cells prevents urea-induced improvement of urinary concentrating ability. Am. J. Physiol. Renal Physiol. 286:F144-F151.
6. Fenton, R. A., C. L. Chou, G. S. Stewart, C. P. Smith, and M. A. Knepper. 2004. Urinary concentrating defect in mice with selective deletion of phloretin-sensitive urea transporters in the renal collecting duct. Proc. Natl. Acad. Sci. USA 101:7469-7474.
7. Fenton, R. A., A. Howorth, G. J. Cooper, R. Meccariello, I. D. Morris, and C. P. Smith. 2000. Molecular characterization of a novel UT-A urea transporter isoform (UT-A5) in testis. Am. J. Physiol. Cell Physiol. 279:C1425-C1431.
8. Fenton, R. A., G. S. Stewart, B. Carpenter, A. Howorth, E. A. Potter, G. J. Cooper, and C. P. Smith. 2002. Characterization of mouse urea transporters UT-A1 and UT-A2. Am. J. Physiol. Renal Physiol. 283:F817-F825.
9. Karakashian, A., R. T. Timmer, J. D. Klein, R. B. Gunn, J. M. Sands, and S. M. Bagnasco. 1999. Cloning and characterization of two new isoforms of the rat kidney urea transporter: UT-A3 and UT-A4. J. Am. Soc. Nephrol. 10:230-237.
10. Klein, J. D., J. M. Sands, L. Qian, X. Wang, and B. Yang. 2004. Upregulation of urea transporter UT-A2 and water channels AQP2 and AQP3 in mice lacking urea transporter UT-B. J. Am. Soc. Nephrol. 15:1161-1167.
11. Knepper, M., and G. Gamba. 2004. Urine concentration and dilution, p. 599-636. In B. Brenner (ed.), Brenner & Rector's the kidney, 7th ed., vol. 1. Saunders, Philadelphia, Pa.
12. Lim, S. W., C. Li, B. K. Sun, K. H. Han, W. Y. Kim, Y. W. Oh, J. U. Lee, P. F. Kador, M. A. Knepper, J. M. Sands, J. Kim, and C. W. Yang. 2004. Long-term treatment with cyclosporine decreases aquaporins and urea transporters in the rat kidney. Am. J. Physiol. Renal Physiol. 287:F139-F151.
13. Matsumura, Y., S. Uchida, Y. Kondo, H. Miyazaki, S. B. Ko, A. Hayama, T. Morimoto, W. Liu, M. Arisawa, S. Sasaki, and F. Marumo. 1999. Overt nephrogenic diabetes insipidus in mice lacking the CLC-K1 chloride channel. Nat. Genet. 21:95-98.
14. Nakayama, Y., M. Naruse, A. Karakashian, T. Peng, J. M. Sands, and S. M. Bagnasco. 2001. Cloning of the rat Slc14a2 gene and genomic organization of the UT-A urea transporter. Biochim. Biophys. Acta 1518:19-26.
15. Nielsen, S., J. Terris, C. P. Smith, M. A. Hediger, C. A. Ecelbarger, and M. A. Knepper. 1996. Cellular and subcellular localization of the vasopressin-regulated urea transporter in rat kidney. Proc. Natl. Acad. Sci. USA 93:5495-5500.
16. Olives, B., P. Neau, P. Bailly, M. A. Hediger, G. Rousselet, J. P. Caitron, and P. Ripoche. 1994. Cloning and functional expression of a urea transporter from human bone marrow cells. J. Biol. Chem. 269:31649-31652.
17. Sands, J. M. 2003. Mammalian urea transporters. Annu. Rev. Physiol. 65:543-566.
18. Sands, J. M. 2004. Renal urea transporters. Curr. Opin. Nephrol. Hypertens. 13:525-532.
19. Shayakul, C., C. P. Smith, H. S. Mackenzie, W. S. Lee, D. Brown, and M. A. Hediger. 2000. Long-term regulation of urea transporter expression by vasopressin in Brattleboro rats. Am. J. Physiol. Renal Physiol. 278:F620-F627.
20. Smith, C. P., W. S. Lee, S. Martial, M. A. Knepper, G. You, J. M. Sands, and M. A. Hediger. 1995. Cloning and regulation of expression of the rat kidney urea transporter (rUT2). J. Clin. Investig. 96:1556-1563.
21. Stewart, G. S., R. A. Fenton, W. Wang, T. H. Kwon, S. J. White, V. M. Collins, G. Cooper, S. Nielsen, and C. P. Smith. 2004. The basolateral expression of mUT-A3 in the mouse kidney. Am. J. Physiol. Renal Physiol. 286:F979-F987.
22. Terris, J. M., M. A. Knepper, and J. B. Wade. 2001. UT-A3: localization and characterization of an additional urea transporter isoform in the IMCD. Am. J. Physiol. Renal Physiol. 280:F325-F332.
23. Timmer, R. T., J. D. Klein, S. M. Bagnasco, J. J. Doran, J. W. Verlander, R. B. Gunn, and J. M. Sands. 2001. Localization of the urea transporter UT-B protein in human and rat erythrocytes and tissues. Am. J. Physiol. Cell Physiol. 281:C1318-C1325.
24. Wade, J. B., A. J. Lee, J. Liu, C. A. Ecelbarger, C. Mitchell, A. D. Bradford, J. Terris, G. H. Kim, and M. A. Knepper. 2000. UT-A2: a 55-kDa urea transporter in thin descending limb whose abundance is regulated by vasopressin. Am. J. Physiol. Renal Physiol. 278:F52-F62.
25. Yang, B., L. Bankir, A. Gillespie, C. J. Epstein, and A. S. Verkman. 2002. Urea-selective concentrating defect in transgenic mice lacking urea transporter UT-B. J. Biol. Chem. 277:10633-10637.
26. You, G., C. P. Smith, Y. Kanai, W. S. Lee, M. Stelzner, and M. A. Hediger. 1993. Cloning and characterization of the vasopressin-regulated urea transporter. Nature 365:844-847.