Importance of 3′-hydroxyl group of the nucleosides for the reactivity of thymidine phosphorylase from Escherichia coli

Chemistry Letters

Volumn 35, Issue 2, 2006, Pages 232-233

  Hatano, Akihiko ^a   Harano, Aiko ^a   Kirihara, Masayuki ^a  

^a SHIZUOKA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 33646458601     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2006.232     Document Type: Article

References (23)

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21. Typical procedure for reaction: To 10 mL of 0.1 M phosphate buffer (pH 7.0), were added thymidine (0.5 mmol, 121 mg), 5-fluorouracil (0.05 mmol, 6.5 mg), and thymidine phosphorylase (SIGMA, 5 units, from Escherichia coli, EC 2.4.2.4). The reaction mixture was stirred at 35 °C for 2 h. The reaction was monitored by HPLC.
22. -1. The mobile phase was 0.10 mM phosphate buffer (pH 6.8). The UV detector was set at 260 nm and the column was operated at 35 °C.
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