A comparison of DNA repair using the comet assay in tobacco seedlings after exposure to alkylating agents or ionizing radiation

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 470, Issue 1, 2000, Pages 1-9

  Gichner, Tomáš ^a   Ptáček, Ondřej ^a   Stavreva, Diana A ^a   Wagner, Elizabeth D ^b   Plewa, Michael J ^b  

^a INSTITUTE OF EXPERIMENTAL BOTANY   (Czech Republic)

^b Department of Animal Sciences   (United States)

Author keywords

radiation; Comet assay; EMS; ENU; Ethyl methanesulfonate; N ethyl N nitrosourea; Nicotiana tabacum var. xanthi; SCGE; Single cell gel electrophoresis

Indexed keywords

ALKYLATING AGENT; ETHYLNITROSOUREA; MESYLIC ACID ETHYL ESTER; MUTAGENIC AGENT; PLANT DNA; TOBACCO;

ARTICLE; BIOLOGICAL MONITORING; CELL NUCLEUS; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; DRUG EXPOSURE; ENVIRONMENTAL MONITORING; GAMMA RADIATION; IONIZING RADIATION; MUTAGENICITY; NONHUMAN; PLANT CELL; PLANT GROWTH; PLANT LEAF; PLANT SEED; PRIORITY JOURNAL; RADIATION EXPOSURE;

ALKYLATING AGENTS; CELL NUCLEUS; COMET ASSAY; DNA; DNA REPAIR; ELECTROPHORESIS; ETHYL METHANESULFONATE; ETHYLNITROSOUREA; GAMMA RAYS; MUTAGENS; PLANT LEAVES; PLANT ROOTS; PLANTS, TOXIC; SUPPORT, NON-U.S. GOV'T; TOBACCO;

EID: 0343714603     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(00)00081-4     Document Type: Article

References (28)

1. R.R. Tice, The single cell gel/comet assay: a microgel electrophoretic technique for the detection of DNA damage and repair in individual cells, in: D.H. Phillips, S. Venitt (Eds.), Environmental Mutagenesis, Bios Scientific Publishers, Oxford, 1995, pp. 315-339.
2. Nocentini S. Comet assay analysis of repair of DNA strand breaks in normal and deficient human cells exposed to radiation and chemicals. Evidence for a repair pathway specificity of DNA ligation. Radiation Res. 144:1995;170-180.
3. Alapetite C., Wachter T., Sage E., Moustacchi E. Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and γ-rays. Int. J. Radiation Biol. 69:1996;359-369.
4. Fortini P., Raspaglio G., Falchi M., Dogliotti E. Analysis of DNA alkylation damage and repair in mammalian cells by the comet assay. Mutagenesis. 11:1996;169-175.
5. Lankinen M.H., Vilpo L.M., Vilpo J.A. UV-and γ-irradiation-induced DNA single-strand breaks and their repair in human blood granulocytes and lymphocytes. Mutat. Res. 352:1996;31-38.
6. Helbig R., Speit G. DNA effects in repair-deficient V79 Chinese hamster cells studied with the comet assay. Mutat. Res. 377:1997;279-286.
7. 2 and γ-irradiation with the alkaline comet assay. Mutat. Res. 383:1997;71-80.
8. McKelvey-Martin V.J., Green M.H.L., Schmezer P., Pool-Zobel B.L., De Méo M.P., Collins A. The single cell gel electrophoresis assay (comet assay): A European review. Mutat. Res. 288:1993;47-63.
9. Constantin M.J., Owens E.T. Introduction and perspectives of plant genetic and cytogenetic assays - A report of the US Environmental Protection Agency Gene-Tox Program. Mutat. Res. 99:1982;1-12.
10. Grant W.F. The present status of higher plant bioassays for the detection of environmental mutagens. Mutat. Res. 310:1994;175-185.
11. Gichner T., Plewa M.J. Induction of somatic DNA damage as measured by single cell gel electrophoresis and point mutation in leaves of tobacco plants. Mutat. Res. 401:1998;143-152.
12. Gichner T., Ptáček O., Stavreva D.A., Plewa M.J. Comparison of DNA damage in plants as measured by single cell gel electrophoresis and somatic leaf mutations induced by monofunctional alkylating agents. Environ. Mol. Mutag. 33:1999;279-286.
13. Dulieu H.L., Dalebroux M.A. Spontaneous and induced reversion rates in a double heterozygous mutant of Nicotiana tabacum var. xanthi n.c.: Dose-response relationship. Mutat. Res. 30:1975;63-70.
14. Murashige T., Skoog F. A revised medium for rapid growth and bio-assays with tobacco cultures. Physiol. Plant. 15:1962;473-497.
15. Lovell D.P., Thomas G., Dubow R. Issues related to the experimental design and subsequent statistical analysis of in vivo and in vitro Comet studies. Teratog. Carcinog. and Mutag. 19:1999;109-119.
16. G.E.P. Box, W.G. Hunter, J.S. Hunter, Statistics for Experimenters: An Introduction to Design, Data Analysis, and Model Building, Wiley, New York, 1978, 653 pp.
17. Miyamae Y., Zaizen K., Ohara K., Mine Y., Sasaki Y.F. Detection of DNA lesions induced by chemical mutagens by the single cell gel electrophoresis (Comet) assay. I. Relationship between the onset of DNA damage and the characteristics of mutagens. Mutat. Res. 415:1998;229-235.
18. Britt A.B. DNA damage and repair in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47:1996;75-100.
19. Vonarx E.J., Mitchell H.L., Karthikeyan R., Chatterjee I., Kunz B.A. DNA repair in higher plants. Mutat. Res. 400:1998;187-200.
20. Koppen G., Angelis K.J. Repair of X-ray induced damage measured by the comet assay in roots of Vicia faba. Environ. Mol. Mutag. 32:1998;281-285.
21. Koppen G., Toncelli L.M., Triest L., Verschaeve L. The comet assay: a tool to study alteration of DNA integrity in developing plant leaves. Mech. Ageing Dev. 110:1999;13-24.
22. R.F. Williams, The Shoot Apex and Leaf Growth, Cambridge University Press, Cambridge, 1975.
23. C.F. Konzak, R.A. Nilan, E.E. Froese-Gertzen, R.J. Foster, Factors affecting the biological action of mutagens, in: J. Veleminsky, T. Gichner (Eds.), Induction of Mutations and the Mutation Process, Academia, Prague, 1964, pp. 123-132.
24. Calleja F., Jansen J.G., Vrieling H., Laval F., van Zeeland A.A. Modulation of the toxic and mutagenic effects induced by methyl methanesulfonate in Chinese hamster ovary cells by overexpression of the rat N-alkylpurine-DNA glycosylase. Mutat. Res. 425:1999;185-194.
25. A.S. Jones, A.M. Mian, R.T. Walker, The alkaline degradation of deoxyribonucleic acid derivatives, J. Chem. Soc. Sect. C 1968 (1968) 2042.
26. Chovanec M., Näslund M., Spivak I., Dušinská M., Cedervall B., Kolman A. Rejoining of DNA strand breaks induced by propylene oxide and epichlorohydrin in human diploid fibroblasts. Environ. Mol. Mutag. 32:1998;223-228.
27. Henderson L., Wolfreys A., Fedyk J., Bourner C., Windebank S. The ability of the Comet assay to discriminate between genotoxins and cytotoxins. Mutagenesis. 13:1998;89-94.
28. Plewa M.J., Wagner E.D., Berhow M.A., Conway A., Rayburn A.L., Anderson D. Antimutagenic activity of chemical fractions isolated from a commercial soybean processing by-product. Teratog., Carcinog. and Mutag. 19:1999;121-135.