The diagnostic accuracy of reverse transcription-PCR quantification of cytokeratin mRNA in the detection of sentinel lymph node invasion in oral and oropharyngeal squamous cell carcinoma: A comparison with immunohistochemistry

Clinical Cancer Research

Volumn 12, Issue 8, 2006, Pages 2498-2505

  Garrel, Renaud ^a   Dromard, Mathilde ^a   Costes, Valérie ^a   Barbotte, Eric ^a   Comte, Frédéric ^a   Gardiner, Quentin ^b   Cartier, César ^a   Makeieff, Marc ^a   Crampette, Louis ^a   Guerrier, Bernard ^a   Boulle, Nathalie ^a  

^a MONTPELLIER UNIVERSITY HOSPITAL   (France)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKERATIN 14; CYTOKERATIN 17; CYTOKERATIN 5; MESSENGER RNA; TUMOR MARKER;

ADULT; AGED; ARTICLE; CANCER DIAGNOSIS; CANCER STAGING; CELL ISOLATION; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION COEFFICIENT; DIAGNOSTIC ACCURACY; FEMALE; HEAD AND NECK CARCINOMA; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INTERMETHOD COMPARISON; MALE; MICROMETASTASIS; MOUTH CANCER; NUCLEOTIDE SEQUENCE; OROPHARYNX CARCINOMA; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SENTINEL LYMPH NODE; SQUAMOUS CELL CARCINOMA; TUMOR BIOPSY;

ADULT; AGED; CARCINOMA, SQUAMOUS CELL; HUMANS; IMMUNOHISTOCHEMISTRY; KERATIN-14; KERATIN-5; KERATINS; LYMPH NODES; LYMPHATIC METASTASIS; MIDDLE AGED; MOUTH NEOPLASMS; NEOPLASM STAGING; OROPHARYNGEAL NEOPLASMS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY; SENTINEL LYMPH NODE BIOPSY;

EID: 33646389526     PISSN: 10780432     EISSN: None     Source Type: Journal    
DOI: 10.1158/1078-0432.CCR-05-2136     Document Type: Article

References (31)

1. Shah JP. Cervical lymph node metastases-diagnostic, therapeutic, and prognostic implications. Oncology (Huntingt) 1990;4:61-9; discussion 72, 76.
2. Kerrebijn JD, Freeman JL, Irish JC, et al. Supraomohyoid neck dissection. Is it diagnostic or therapeutic? Head Neck 1999;21:39-42.
3. 0 neck? Head Neck 2004;26:603-11.
4. Ross GL, Soutar DS, MacDonald DG, et al. Improved staging of cervical metastases in clinically node-negative patients with head and neck squamous cell carcinoma. Ann Surg Oncol 2004;11:213-8.
5. van Diest PJ, Torrenga H, Meijer S, Meijer CJ. Pathologic analysis of sentinel lymph nodes. Semin Surg Oncol 2001;20:238-45.
6. Hermanek P, Hutter RV, Sobin LH, Wittekind C. International Union Against Cancer. Classification of isolated tumor cells and micrometastasis. Cancer 1999;86:2668-73.
7. 0 squamous cell carcinoma of the oral cavity and oropharynx. Otolaryngol Head Neck Surg 2005;132:99-102.
8. Asthana S, Deo SV, Shukla NK, et al. Intraoperative neck staging using sentinel node biopsy and imprint cytology in oral cancer. Head Neck 2003;25:368-72.
9. Raja S, El-Hefnawy T, Kelly LA, et al. Temperature-controlled primer limit for multiplexing of rapid, quantitative reverse transcription-PCR assays: application to intraoperative cancer diagnostics. Clin Chem 2002;48:1329-37.
10. Hamakawa H, Onishi A, Sumida T, et al. Intraoperative real-time genetic diagnosis for sentinel node navigation surgery. Int J Oral Maxillofac Surg 2004;33:670-5.
11. Ferris RL, Xi L, Raja S, et al. Molecular staging of cervical lymph nodes in squamous cell carcinoma of the head and neck. Cancer Res 2005;65:2147-56.
12. Kataoka A, Mori M, Sadanaga N, et al. RT-PCR detection of breast cancer cells in sentinel lymph modes. Int J Oncol 2000;16:1147-52.
13. Blaheta HJ, Schittek B, Breuninger H, Garbe C. Detection of micrometastasis in sentinel lymph nodes of patients with primary cutaneous melanoma. Recent Results Cancer Res 2001;158:137-46.
14. Hamakawa H, Fukuzumi M, Bao Y, et al. Keratin mRNA for detecting micrometastasis in cervical lymph nodes of oral cancer. Cancer Lett 2000;160:115-23.
15. Becker MT, Shores CG, Yu KK, Yarbrough WG. Molecular assay to detect metastatic head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 2004;130:21-7.
16. Shores CG, Yin X, Funkhouser W, Yarbrough W. Clinical evaluation of a new molecular method for detection of micrometastases in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 2004;130:937-42.
17. Garrel R, Makeieff M, Alovisetti C, et al. Sentinel lymph node in oropharyngeal and oral carcinomas. Fr ORL 2005;88:108-15.
18. Ross GL, Shoaib T, Soutar DS, et al. The First International Conference on Sentinel Node Biopsy in Mucosal Head and Neck Cancer and adoption of a multicenter trial protocol. Ann Surg Oncol 2002;9:406-10.
19. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982;31:11-24.
20. Cohen-Kerem R, Madah W, Sabo E, et al. Cytokeratin-17 as a potential marker for squamous cell carcinoma of the larynx. Ann Otol Rhinol Laryngol 2004;113:821-7.
21. Chu PG, Weiss LM. Keratin expression in human tissues and neoplasms. Histopathology 2002;40:403-39.
22. Miettinen M, Fetsch JF. Distribution of keratins in normal endothelial cells and a spectrum of vascular tumors: implications in tumor diagnosis. Hum Pathol 2000;31:1062-7.
23. Copois V, Bret C, Bibeau F, et al. Assessment of RNA quality extracted from laser-captured tissues using miniaturized capillary electrophoresis. Lab Invest 2003;83:599-602.
24. Hanley JA, McNeil BJ. The meaning and use of the area under a receiver operating characteristic (ROC) curve. Radiology 1982;143:29-36.
25. DeLong ER, DeLong DM, Clarke-Pearson DL. Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach. Biometrics 1988;44:837-45.
26. Swets JA. Measuring the accuracy of diagnostic systems. Science 1988;240:1285-93.
27. Ross GL, Soutar DS, Gordon MacDonald D, et al. Sentinel node biopsy in head and neck cancer: preliminary results of a multicenter trial. Ann Surg Oncol 2004;11:690-6.
28. Romanini A, Manca G, Pellegrino D, et al. Molecular staging of the sentinel lymph node in melanoma patients: correlation with clinical outcome. Ann Oncol 2005;16:1832-40.
29. Stoeckli SJ, Pfaltz M, Steinert H, Schmid S. Histopathological features of occult metastasis detected by sentinel lymph node biopsy in oral and oropharyngeal squamous cell carcinoma. Laryngoscope 2002;112:111-5.
30. Rhee D, Wenig BM, Smith RV. The significance of immunohistochemically demonstrated nodal micrometastases in patients with squamous cell carcinoma of the head and neck. Laryngoscope 2002;112:1970-4.
31. Robbins KT, Clayman GL, Levine PA, et al. Neck dissection classification update: revisions proposed by the American Head and Neck Society and the American Academy of Otolaryngology-Head and Neck surgery. Arch Otolaryngol Head Neck Surg 2002;128:751-8.