Temperature-controlled primer limit for multiplexing of rapid, Quantitative Reverse Transcription-PCR assays: Application to intraoperative cancer diagnostics

Clinical Chemistry

Volumn 48, Issue 8, 2002, Pages 1329-1337

  Raja, Siva ^a   El Hefnawy, Talal ^a   Kelly, Lori A ^a   Chestney, Melissa L ^a   Luketich, James D ^a   Godfrey, Tony E ^a  

^a PRESBYTERIAN UNIVERSITY HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARCINOEMBRYONIC ANTIGEN; MESSENGER RNA; MONOPHENOL MONOOXYGENASE; PRIMER DNA;

ANALYTIC METHOD; ARTICLE; CANCER STAGING; CHEMICAL REACTION; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC KIT; ESOPHAGUS CARCINOMA; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; INTRAOPERATIVE PERIOD; LYMPH NODE BIOPSY; LYMPH NODE METASTASIS; QUANTITATIVE DIAGNOSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SIGNAL DETECTION; TEMPERATURE DEPENDENCE; TIME;

CEPHEIDAE;

EID: 0036323156     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/48.8.1329     Document Type: Article

References (30)

1. Detection of arboviral RNA directly from mosquito homogenates by reverse-transcription-polymerase chain reaction
2. High-throughput detection of West Nile virus RNA
3. Quantitative, real-time polymerase chain reactions for FLT3 internal tandem duplications are highly sensitive and specific
4. Real-time quantitative PCR for detection of minimal residual disease before allogeneic stem cell transplantation predicts outcome in children with acute lymphoblastic leukemia
5. Prognostic value of minimal residual disease in relapsed childhood acute lymphoblastic leukaemia
6. Rapid detection of group B streptococci in pregnant women at delivery
7. Development of a PCR assay for rapid detection of enterococci
8. Prognostic value of quantitative reverse transcription-polymerase chain reaction in lymph node-negative esophageal cancer patients
9. Micrometastases and survival in stage II colorectal cancer
10. Molecular staging of malignant melanoma: Correlation with clinical outcome
11. Prognostic significance of occult metastases detected by sentinel lymphadenectomy and reverse transcriptase-polymerase chain reaction in early-stage melanoma patients
12. A randomized trial comparing perioperative chemotherapy and surgery with surgery alone in resectable stage IIIa non-small-cell lung cancer
13. Preoperative chemotherapy for stage IIIa (N2) lung cancer: The Sloan-Kettering experience with 136 patients
14. Preresectional chemotherapy in stage IIIA non-small-cell lung cancer: A 7-year assessment of a randomized controlled trial
15. Endoscopic ultrasound-guided fine needle aspiration for staging patients with carcinoma of the lung
16. Is routine intraoperative frozen-section examination of sentinel lymph nodes in breast cancer worthwhile?
17. Recent developments in the molecular characterization of Barrett's esophagus
18. Quantitative real-time RT-PCR detection of breast cancer micrometastasis using a multigene marker panel
19. Rapid, quantitative reverse transcriptase-polymerase chain reaction: Application to intraoperative molecular detection of occult metastases in esophageal cancer
20. Development of internal controls for PCR detection of Bacillus anthracis
21. An internal control for routine diagnostic PCR: Design, properties, and effect on clinical performance
22. A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR
23. Quantitative mRNA expression analysis from formalin-fixed, paraffin-embedded tissues using 5′ nuclease quantitative reverse transcription-polymerase chain reaction
24. Increased sensitivity of one-tube, quantitative RT-PCR
25. Interinstitutional comparison of frozen section turnaround time. A College of American Pathologists Q-Probes study of 32868 frozen sections in 700 hospitals
26. The PCR plateau pH
27. Development of a highly sensitive nested-PCR procedure using a single closed tube for detection of Erwinia amylovora in asymptomatic plant material
28. Quantitation of intratumoral thymidylate synthase expression predicts for disseminated colorectal cancer response and resistance to protracted-infusion fluorouracil and weekly leucovorin
29. Anticancer drugs acting against signaling pathways
30. Antitumor effect and potentiation of cytotoxic drugs activity in human cancer cells by ZD-1839 (Iressa), an epidermal growth factor receptor-selective tyrosine kinase inhibitor