Development of a time-resolved fluorometric assay for the high throughput screening of melanin concentrating hormone receptor antagonists

Journal of Pharmacological and Toxicological Methods

Volumn 53, Issue 3, 2006, Pages 242-247

  Lee, Sunghou ^a   Kim, Gun Do ^c   Park, Woo Kyu ^b   Cho, Heeyeong ^b   Byung Ho Lee, ^b   Yoo, Sung eun ^b   Jae Yang Kong, ^b  

^a Sangmyung University   (South Korea)

^b Korea Research Institute of Chemical Technology   (South Korea)

^c Pukyong National University   (South Korea)

Author keywords

AcroWell; Filter plate; MCH; Melanin concentrating hormone; Methods; Receptor binding assay; Time resolved; TRF

Indexed keywords

HORMONE RECEPTOR BLOCKING AGENT; MELANIN CONCENTRATING HORMONE RECEPTOR ANTAGONIST; METHYL 3 [[[3 [4 [3 (ACETYLAMINO)PHENYL] 1 PIPERIDINYL]PROPYL]AMINO] CARBONYL] 4 (3,4 DIFLUOROPHENYL) 6 (METHOXYMETHYL) 2 OXO 1,2,3,4 TETRAHYDRO 5 PYRIMIDINECARBOXYLATE; RADIOISOTOPE; RADIOLIGAND; SNAP 7941; UNCLASSIFIED DRUG;

ARTICLE; BINDING ASSAY; CORRELATION COEFFICIENT; DRUG RECEPTOR BINDING; DRUG SCREENING; FILTER; FLUOROMETRY; HIGH THROUGHPUT SCREENING; HUMAN; IC 50; LIGAND BINDING; MOUSE; NONHUMAN; RADIOASSAY; RAT; REPRODUCIBILITY; SENSITIVITY ANALYSIS; SIGNAL NOISE RATIO; VALIDATION PROCESS;

ANIMALS; BIOLOGICAL ASSAY; DOSE-RESPONSE RELATIONSHIP, DRUG; FLUOROMETRY; HUMANS; INHIBITORY CONCENTRATION 50; LIGANDS; MELANINS; MICE; PIPERIDINES; PYRIMIDINES; RADIOLIGAND ASSAY; RATS; RECEPTORS, PITUITARY HORMONE; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 33646266742     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vascn.2005.09.001     Document Type: Article

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