Caldesmon transgene expression disrupts focal adhesions in HTM cells and increases outflow facility in organ-cultured human and monkey anterior segments

Experimental Eye Research

Volumn 82, Issue 6 SPEC. ISS., 2006, Pages 935-944

  Gabelt, B'Ann True ^a   Hu, Yujie ^a   Vittitow, Jason L ^b   Rasmussen, Carol R ^a   Grosheva, Inna ^c   Bershadsky, Alexander D ^c   Geiger, Benjamin ^c   Borrás, Terete ^b   Kaufman, Paul L ^a  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

actin cytoskeleton; caldesmon; focal adhesions; gene therapy; organ cultured anterior segments; outflow facility; trabecular meshwork; vinculin

Indexed keywords

ACTIN; ADENOVIRUS VECTOR; CALDESMON; VINCULIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOSKELETON; EMBRYO; EXTRACELLULAR MATRIX; FOCAL ADHESION; GENE EXPRESSION; GENE THERAPY; GLAUCOMA; HUMAN; HUMAN CELL; HUMAN TISSUE; MONKEY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; STRESS FIBER; TRABECULAR MESHWORK; TRANSGENE; VIRAL GENE DELIVERY SYSTEM;

EID: 33646200546     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exer.2005.12.002     Document Type: Article

References (40)

1. Bahler C.K., Hann C.R., Fautsch M.P., and Johnson D.H. Pharmacologic disruption of Schlemm's canal cells and outflow facility in anterior segments of human eyes. Invest. Ophthalmol. Vis. Sci. 45 (2004) 2246-2254
2. Bárány E.H. Simultaneous measurements of changing intraocular pressure and outflow facility in the vervet monkey by constant pressure infusion. Invest. Ophthalmol. 3 (1964) 135-143
3. Bershadsky A., Chausovsky A., Becker E., Lyubimova A., and Geiger B. Involvement of microtubules in the control of adhesion-dependent signal transduction. Curr. Biol. 6 (1996) 1279-1289
4. Borrás T., Tamm E., and Zigler Jr. J.S. Ocular adenovirus gene transfer varies in efficiency and inflammatory response. Invest. Ophthalmol. Vis. Sci. 37 (1996) 1282-1293
5. Borrás T., Masumoto Y., Epstein D.L., and Johnson D.H. Gene transfer to the human trabecular meshwork by anterior segment perfusion. Invest. Ophthalmol. Vis. Sci. 39 (1998) 1503-1507
6. Borrás T., Rowlette L.L., Erzurum S.C., and Epstein D.L. Adenoviral reporter gene transfer to the human trabecular meshwork does not alter aqueous humor outflow. Relevance for potential gene therapy of glaucoma. Gene Ther. 6 (1999) 515-524
7. Borrás T., Gabelt B.T., Klintworth G.K., Peterson J.C., and Kaufman P.L. Non-invasive observation of repeated adenoviral GFP gene delivery to the anterior segment of the monkey eye in vivo. J. Gene Med. 3 (2001) 437-449
8. Cai S., Liu X., Glasser A., Volberg T., Filla M., Geiger B., and Kaufman P.L. Effect of latrunculin-A on morphology and actin-associated adhesions of cultured human trabecular meshwork cells. Mol. Vis. 6 (2000) 132-143
9. Chalovich J.M., Sen A., Resetar A., Leinweber B., Fredricksen R.S., Lu F., and Chen Y.D. Caldesmon: binding to actin and myosin and effects on elementary steps in the ATPase cycle. Acta Physiol. Scand. 164 (1998) 427-435
10. Chrzanowska-Wodnicka M., and Burridge K. Rho-stimulated contractility drives the formation of stress fibers and focal adhesions. J. Cell Biol. 133 (1996) 1403-1415
11. Comes N., Gasuli X., Gual A., and Borras T. Differential expression of the human chloride channel genes in the trabecular meshwork under stress conditions. Exp. Eye Res. 80 (2005) 801-813
12. Epstein D.L., Rowlette L.-L., and Roberts B.C. Acto-myosin drug effects and aqueous outflow function. Invest. Ophthalmol. Vis. Sci. 40 (1999) 74-81
13. Gabelt B.T., Gottanka J., Lütjen-Drecoll E., and Kaufman P.L. Aqueous humor dynamics and trabecular meshwork and anterior ciliary muscle morphologic changes with age in rhesus monkeys. Invest. Ophthalmol. Vis. Sci. 44 (2003) 2118-2125
14. Goldberg I. Compliance with medical therapy in chronic glaucoma. In: Gramer E., and Grehn F. (Eds). Pathogenesis and Risk Factors of Glaucoma (1999), Springer, Berlin 25-33 (Chapter 4)
15. Gonzalez P., Caballero M., Liton P.B., Stamer W.D., and Epstein D.L. Expression analysis of the matrix GLA protein and VE-cadherin gene promotoers in the outflow pathway. Invest. Ophthalmol. Vis. Sci. 45 (2004) 1389-1395
16. Grierson I., and Howes R.C. Age-related depletion of the cell population in the human trabecular meshwork. Eye 1 (1987) 204-210
17. Helfman D.M., Lemy E.T., Berthier C., Shtutman M., Riveline D., Grosheva I., Lachish-Zalait A., Elbaum M., and Bershadsky A.D. Caldesmon inhibits nonmuscle cell contractility and interferes with the formation of focal adhesions. Mol. Biol. Cell 10 (1999) 3097-3112
18. Honjo M., Tankhara H., Inatani M., Kido N., Sawamura T., Yue B.Y.J.T., Narumiya S., and Honda Y. Effects of rho-associated protein kinase inhibitor Y-27632 on intraocular pressure and outflow facility. Invest. Ophthalmol. Vis. Sci. 42 (2001) 137-144
19. Huber P.A. Caldesmon. Int. J. Biochem. Cell Biol. 29 (1997) 1047-1051
20. Ito S., and Karnovsky M.J. Formaldehyde-glutaraldehyde fixatives containing trinitro compounds. J. Cell Biol. 39 (1968) 168a-169a (Abs nr 418)
21. Johnson D.H., and Tschumper R.C. Human trabecular meshwork organ culture: a new model. Invest. Ophthalmol. Vis. Sci. 28 (1987) 945-953
22. Kaufman P.L., Tian B., Gabelt B.T., and Liu X. Outflow enhancing drugs and gene therapy in glaucoma. In: Weinreb R., Krieglstein G., and Kitazawa Y. (Eds). Glaucoma in the 21st Century (2000), Harcourt/Mosby, London 117-128 (Chapter 17)
23. Liton P.B., Liu X., Stamer W.D., Challa P., Epstein D.L., and Gonzalez P. Specific targeting of gene expression to a subset of human trabecular meshwork cells using the chitinase 3-like 1 promoter. Invest. Ophthalmol. Vis. Sci. 46 (2005) 183-190
24. Liu, X., Hu, Y., Filla, M.S., Gabelt, B.T., Peters, D.M., Brandt, C.R., Kaufman, P.L., 2005. The effects of C3 transgene expression on actin and cellular adhesions in cultured HTM cells and on outflow facility in organ cultured monkey eyes. Mol. Vis. 11, 1112-1121.
25. Marston S., Burton D., Copeland O., Fraser I., Gao Y., Hodgkinson J., Huber P., Levine B., El-Mezgueidi M., and Notarianni G. Structural interactions between actin, tropomyosin, caldesmon and calcium binding protein and the regulation of smooth muscle thin filaments. Acta Physiol. Scand. 164 (1998) 401-414
26. Matsumura F., and Yamashiro S. Caldesmon. Curr. Opin. Cell Biol. 5 (1993) 70-76
27. Pelham R.J., and Wang Y.-L. Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc. Natl Acad. Sci. USA 94 (1997) 13661-13665
28. Peterson J.A., Tian B., Bershadsky A.D., Volberg T., Gangnon R.E., Spector I., Geiger B., and Kaufman P.L. Latrunculin-A increases outflow facility in the monkey. Invest. Ophthalmol. Vis. Sci. 40 (1999) 931-941
29. Rao P.V., Deng P.-F., Kumar J., and Epstein D.L. Modulation of aqueous humor outflow facility by the rho kinase-specific inhibitor Y-27632. Invest. Ophthalmol. Vis. Sci. 42 (2001) 1029-1037
30. Ritch R., Shields M.B., and Krupin T. Chronic open-angle glaucoma: treatment overview. In: Ritch R., Shields M.B., and Krupin T. (Eds). The Glaucomas. second ed. (1996), Mosby, St Louis, MO 1507-1519 (Chapter 73)
31. Riveline D., Zamir E., Balaban N.Q., Schwarz U.S., Ishizaki T., Marumiya S., Kam Z., Geiger B., and Bershadsky A.D. Focal contacts as mechanosensors: externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism. J. Cell Biol. 153 (2001) 1175-1186
32. Sabanay I., Gabelt B.T., Tian B., Kaufman P.L., and Geiger B. H-7 effects on structure and fluid conductance of monkey trabecular meshwork. Arch. Ophthalmol. 118 (2000) 955-962
33. Sabanay I., Tian B., Gabelt B.T., Geiger B., and Kaufman P.L. Functional and structural reversibility of H-7 effects on the conventional aqueous outflow pathway in monkeys. Exp. Eye Res. 78 (2004) 137-150
34. Sabanay, I., Tian, B., Gabelt, B.T., Geiger, B., Kaufman, P.L., in press. Latrunculin B effects on trabecular meshwork and corneal endothelium structure in the monkey eye. Exp. Eye Res.
35. Tian B., Kaufman P.L., Volber T., Gabelt B.T., and Geiger B. H-7 disrupts the actin cytoskeleton and increases outflow facility. Arch. Opthalmol. 116 (1998) 633-643
36. Tian B., Brumback L.C., and Kaufman P.L. ML-7, chelerythrine and phorbol ester increase outflow facility in the monkey eye. Exp. Eye Res. 71 (2000) 551-566
37. Tian B., Wang R.-F., Podos S.M., and Kaufman P.L. Effects of topical H-7 on outflow facility, intraocular pressure and corneal thickness in monkeys. Arch. Ophthalmol. 122 (2004) 1171-1177
38. Vittitow J.L., Garg R., Rowlette L.L., Epstein D.L., O'Brien E.T., and Borrás T. Gene transfer of dominant-negative RhoA increases outflow facility in perfused human anterior segment cultures. Mol. Vis. 8 (2002) 32-44
39. Vittitow J.L., Grosheva I., Bershadsky A.D., Geiger B., Kaufman P.L., and Borrás T. Effect of rat non-muscle caldesmon overexpression on the cytoskeleton of human trabecular meshwork cells. Invest. Ophthalmol. Vis. Sci. 45 (2004) (Abs nr 4386)
40. Volberg T., Geiger B., Citi S., and Bershadsky A.D. Effect of protein kinase inhibitor H-7 on the contractility, integrity and membrane anchorage of the microfilament system. Cell Motil. Cytoskeleton 29 (1994) 321-338