Probing the sterol binding site of soybean sterol methyltransferase by site-directed mutagenesis: Functional analysis of conserved aromatic amino acids in Region 1

Archives of Biochemistry and Biophysics

Volumn 448, Issue 1-2, 2006, Pages 23-30

  Nes, W David ^a   Sinha, Archana ^a   Jayasimha, Pruthvi ^a   Zhou, Wenxu ^a   Song, Zhihong ^a   Dennis, Allen L ^a  

^a TEXAS TECH UNIVERSITY   (United States)

Author keywords

C Methylation; Cycloartenol; Glycine max; Site directed mutagenesis; Sitosterol; Soybean phytosterols; Sterol methyltransferase; Tyrosine

Indexed keywords

ACETAL; AROMATIC AMINO ACID; CYCLOARTENOL; HYDROXYL GROUP; ISOLEUCINE; LEUCINE; METHYLTRANSFERASE; MUTANT PROTEIN; PHENYLALANINE; SITOSTEROL; STEROL;

ANIMAL CELL; ARTICLE; BACTERIAL CELL; BINDING SITE; CATALYSIS; CHEMICAL REACTION; CIRCULAR DICHROISM; FUNCTIONAL ASSESSMENT; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; METHYLATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; SOYBEAN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; CONSERVED SEQUENCE; ESCHERICHIA COLI; HYDROCARBONS, AROMATIC; METHYLTRANSFERASES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; RECOMBINANT PROTEINS; SOYBEANS; STEROLS;

ANIMALIA; BACTERIA (MICROORGANISMS); GLYCINE MAX;

EID: 33646152537     PISSN: 00039861     EISSN: 10960384     Source Type: Journal    
DOI: 10.1016/j.abb.2005.08.022     Document Type: Article

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