Immunity, Pathophysiology, and Diagnosis of Equine Protozoal Myeloencephalitis

Clinical Techniques in Equine Practice

Volumn 5, Issue 1, 2006, Pages 3-8

  Furr, Martin ^a  

^a MARION DUPONT SCOTT EQUINE MEDICAL CENTER   (United States)

Author keywords

equine protozoal myeloencephalitis; indirect fluorescent antibody test; Sarcocystis neurona; Western blot

Indexed keywords

EQUIDAE; SARCOCYSTIS NEURONA;

EID: 33646109056     PISSN: 15347516     EISSN: None     Source Type: Journal    
DOI: 10.1053/j.ctep.2006.01.002     Document Type: Article

References (58)

1. Bentz B.G., Ealey K.A., Morrow J., et al. Seroprevalence of antibodies to Sarcocystis neurona in equids residing in Oklahoma. J Vet Diagn Invest 15 (2003) 597-600
2. Blythe L.L., Granstrom D.E., Hansen D.E., et al. Seroprevalence of antibodies to Sarcocystis neurona in horses residing in Oregon. J Am Vet Med Assoc 210 (1997) 525-527
3. Saville W.J., Reed S.M., Granstrom D.E., et al. Seroprevalence of antibodies to Sarcocystis neurona in horses residing in Ohio. J Am Vet Med Assoc 210 (1997) 519-524
4. Dubey J.P., Lindsay D.S., Saville W.J., et al. A review of Sarcocystis neurona and equine protozoal myeloencephalitis (EPM). Vet Parasitol 95 (2001) 89-131
5. Sofaly C.D., Reed S.M., Gordon J.C., et al. Experimental induction of equine protozoan myeloencephalitis (EPM) in the horse. effect of Sarcocystis neurona sporocyst inoculation dose on the development of clinical neurologic disease. J Parasitol 88 (2002) 1164-1170
6. Saville W.J., Reed S.M., Morley P.S., et al. Analysis of risk factors for the development of equine protozoal myeloencephalitis in horses. J Am Vet Med Assoc 217 (2000) 1174-1180
7. Saville W.J., Stich R.W., Reed S.M., et al. Utilization of stress in the development of an equine model for equine protozoal myeloencephalitis. Vet Parasitol 95 (2001) 211-222
8. Cutler T.J., MacKay R.J., Ginn P.E., et al. Immunoconversion against Sarcocystis neurona in normal and dexamethasone-treated horses challenged with S. neurona sporocysts. Vet Parasitol 95 (2001) 197-210
9. Njoku C.J., Saville W.J., Reed S.M., et al. Reduced levels of nitric oxide metabolites in cerebrospinal fluid are associated with equine protozoal myeloencephalitis. Clin Diagn Lab Immunol 9 (2002) 605-610
10. Dubey J.P. Migration and development of Sarcocystis neurona in tissues of interferon gamma knockout mice fed sporocysts from a naturally infected opossum. Vet Parasitol 95 (2001) 341-351
11. Sellon D.C., Knowles D.P., Greiner E.C., et al. Infection of immunodeficient horses with Sarcocystis neurona does not result in neurologic disease. Clin Diagn Lab Immunol 11 (2004) 1134-1139
12. Rossano M.G., Schott II H.C., Murphy A.J., et al. Parasitemia in an immunocompetent horse experimentally challenged with Sarcocystis neurona sporocysts. Vet Parasitol 127 (2005) 3-8
13. Ellison S.P., Kennedy T.J., and Brown K.K. Early signs of equine protozoal myeloencephalitis. J Appl Res Vet Med 1 (2003) 272-278
14. Lindsay D.S., Mitchell S.M., Yang J., et al. Penetration of equine leukocytes by merozoites of Sarcocystis neurona. ultrastructural evidence for possible transport mechanism for equine protozoal myeloencephalitis. Proceedings of the 50th Annual Meeting of the American Association of Veterinary Parasitology (2005) p 45 (abstr)
15. Witonsky S.G., Gogal Jr. R.M., Duncan R.B., et al. Protective immune response to experimental infection with Sarcocystis neurona in 57BL/6 mice. J Parasitol 89 (2003) 924-931
16. Alvers M.J., Abuin G., Kuwajima V.Y., et al. Partial inhibition of trypomastigote entry into cultured mammalian cells by monoclonal antibodies against a surface glycoprotein of Trypanosoma cruzi. Mol Biochem Parasitol 21 (1986) 75-82
17. Tzipori S., Robertson D., Cooper D., et al. Chronic cryptosporidiosis and hyperimmune bovine colostrum. Lancet ii (1987) 344-345
18. Doyle P.S., Crabb J., and Peterson C. Anti-Cryptosporidium parvum antibodies inhibit infectivity in vitro and in vivo. Infect Immun 61 (1993) 4079-4084
19. Liang F.T., Granstrom D.E., Zhao X.M., et al. Evidence that surface proteins Sn14 and Sn16 of Sarcocystis neurona merozoites are involved in infection and immunity. Infect Immun 66 (1998) 1834-1838
20. Furr M., Pontzer C., and Gasper P. Lymphocyte phenotype subsets in the cerebrospinal fluid of normal horses and horses with equine protozoal myeloencephalitis. Vet Ther 2 (2001) 317-324
21. Tornquist S.J., Boeder L.J., Mattson D.E., et al. Lymphocyte responses and immunophenotypes in horses with Sarcocystis neurona infection. Equine Vet J 33 (2001) 726-729
22. Witonsky S.G., Gogal Jr. R.M., Duncan Jr. R.B., et al. Prevention of meningo/encephalomyelitis due to Sarcocystis neurona infection in mice is mediated by CD8 cells. Int J Parasitol 35 (2005) 113-123
23. Suzuki Y., Orrelman M., Schreiber R., et al. Interferon-γ. the major mediator of resistance against Toxoplasma gondii. Science 240 (1988) 516-518
24. Dubey J.P., and Lindsay D.S. Isolation in immunodeficient mice of Sarcocystis neurona from opossum (Didelphis virginiana) faeces, and its differentiation from Sarcocystis falcatula. Int J Parasitol 28 (1998) 1823-1828
25. Sellon D.C., Knowles D.P., Greiner E.C., et al. Depletion of natural killer cells does not result in neurologic disease due to Sarcocystis neurona in mice with severe combined immunodeficiency. J Parasitol 90 (2004) 782-788
26. Denkers E.Y., Gassinelli R.T., Martin C., et al. Emergence of NK1.1+ cells as effectors of IFN-gamma dependent immunity to Toxoplasma gondii in MHC class I-deficient mice. J Exp Med 178 (1993) 1465-1472
27. Dawson V.L., and Dawson T.M. Nitric oxide actions in neurochemistry. Neurchem Int 29 (1996) 97-110
28. Stich R.W., Shoda L., Dreewes M., et al. Stimulation of nitric oxide production in macrophages by Babesia bovis. Infect Immun 66 (1998) 4130-4136
29. Metz G., Carlier Y., and Vray B. Trypanosoma cruzi upregulates nitric oxide release by IFN-gamma-preactived macrophages, limiting cell infection independently of the respiratory burst. Parasite Immunol 15 (1993) 693-699
30. Shoda L., Kegerreis K., Suarez E., et al. DNA from protozoan parasites Babesia bovis, Trypanosoma cruzi, and T. Brucei is mitogenic for B lymphocytes and stimulates macrophage expression of interleukin-12, tumor necrosis factor alpha, and nitric oxide. Infect Immun 69 (2001) 2162-2171
31. Rosypal A.C., Lindsay D.S., Duncan Jr. R., et al. Mice lacking the gene for inducible or endothelial nitric oxide are resistant to sporocyst induced Sarcocystis neurona infections. Vet Parasitol 103 (2002) 315-321
32. Spencer J.A., Ellison S.E., Guarino A.J., et al. Cell-mediated immune responses in horses with equine protozoal myeloencephalitis. J Parasitol 90 (2004) 428-430
33. Cserr H.F., and Knopf P.M. Cervical lymphatics, the blood-brain barrier and the immunoreactivity of the brain. a new view. Immunol Today 13 (1992) 507-512
34. Taylor A.W. Neuroimmunomodulation in immune privilege. Role of neuropeptides in ocular immunosuppression. Neuroimmunomodulation 3 (1996) 195-204
35. Taylor A., and Streilein J. Inhibition of antigen stimulated effector T cells by human cerebrospinal fluid. Neuroimmunomodulation 3 (1996) 112-118
36. Wilbanks G., and Streilein J. Fluids from immune privileged sites endow macrophages with the capacity to induce antigen-specific immune deviation via a mechanism involving transforming growth factor-β. Eur J Immunol 22 (1992) 1031-1036
37. Silva J.C., Twardzik D., and Reed S. Regulation of Trypanosoma cruzi infection in vitro and in vivo by transforming growth factor β (TGF-β). J Exp Med 174 (1991) 539-545
38. Letterio J., and Roberts A. TGF-β; a critical modulator of immune cell function. Clin Immunol Immunopathol 84 (1997) 244-250
39. Furr M., and Pontzer C. Transforming growth factor beta concentrations and interferon gamma responses in cerebrospinal fluid of horses with equine protozoal myeloencephalitis. Equine Vet J 33 (2001) 721-725
40. Furr M., MacKay R., Granstrom D., et al. Clinical diagnosis of equine protozoal myeloencephalitis (EPM). J Vet Intern Med 16 (2002) 618-621
41. Furr M.O., and Tyler R.D. Cerebrospinal fluid creatine kinase activity in horses with central nervous system disease. 69 cases (1984-1989). J Am Vet Med Assoc 197 (1990) 245-248
42. Jackson C., DeLahunta A., Divers T.J., et al. The diagnostic utility of cerebrospinal fluid creatine kinase activity in the horse. J Vet Intern Med 10 (1996) 246-251
43. Granstrom D. Diagnosis of equine protozoal myeloencephalitis. western blot analysis. Proceedings of the American College of Veterinary Internal Medicine Forum (1993) 587-590
44. Bentz B.G., Carter W.G., and Tobin T. Diagnosing equine protozoal myeloencephalitis. complicating factors. Compend Contin Educ Pract Vet 21 (1999) 975-981
45. Cook A.G., Maxwell V.B., Donaldson L.L., et al. Detection of antibodies against Sarcocystis neurona in cerebrospinal fluid from clinically normal neonatal foals. J Am Vet Med Assoc 220 (2002) 208-211
46. Andrews F., Maddux J., and Faulk B. Total protein, albumin quotient, IgG and IgG index determinations for horse cerebrospinal fluid. Prog Vet Neur 1 (1990) 197-204
47. Miller M.M., Sweeney C.R., Russell G.E., et al. Effects of blood contamination of cerebrospinal fluid on western blot analysis for detection of antibodies against Sarcocystis neurona and on albumin quotient and immunoglobulin G index in horses. J Am Vet Med Assoc 215 (1999) 67-71
48. Furr M. Antigen-specific antibodies in cerebrospinal fluid after intramuscular injection of ovalbumin in horses. J Vet Intern Med 16 (2002) 588-592
49. Andrews F.M., Latimer F., Grubbs S., et al. CSF indices after repeated spinal taps in horses diagnosed with equine protozoal myeloencephalitis. Proc Ann Conv Am Assoc Equine Pract 43 (1997) 10-12
50. Furr M., Kennedy T., MacKay R., et al. Efficacy of ponazuril 15% oral paste as a treatment for equine protozoal myeloencephalitis. Vet Ther 2 (2001) 215-222
51. Rossano M.G., Mansfield L.S., Kaneene J.B., et al. Improvement of western blot test specificity for detecting equine serum antibodies to Sarcocystis neurona. J Vet Diagn Invest 12 (2000) 28-32
52. Duarte P.C., Daft B.M., Conrad P.A., et al. Comparison of a serum indirect fluorescent antibody test with two Western blot tests for the diagnosis of equine protozoal myeloencephalitis. J Vet Diagn Invest 15 (2003) 8-13
53. Duarte P.C., Daft B.M., Conrad P.A., et al. Evaluation and comparison of an indirect fluorescent antibody test for detection of antibodies to Sarcocystis neurona, using serum and cerebrospinal fluid of naturally and experimentally infected, and vaccinated horses. J Parasitol 90 (2004) 379-386
54. Granstrom D.E., Dubey J.P., Davis S.W., et al. Equine protozoal myeloencephalitis. antigen analysis of cultured Sarcocystis neurona merozoites. J Vet Diagn Invest 5 (1993) 88-90
55. Saville W.J., Dubey J.P., Oglesbee M.J., et al. Experimental infection of ponies with Sarcocystis fayeri and differentiation from Sarcocystis neurona infections in horses. J Parasitol 90 (2004) 1487-1491
56. Hoane J.S., Morrow J., Saville W.J., et al. Enzyme-linked immunosorbent assays for detection of equine antibodies specific to Sarcocystis neurona surface antigens. Clin Diagn Lab Immunol 12 (2005) 1050-1056
57. Hyun C., Gupta G.D., and Marsh A.E. Sequence comparison of Sarcocystis neurona surface antigen from multiple isolates. Vet Parasitol 112 (2003) 11-20
58. Marsh A.E., Johnson P.J., Ramos-Vara J., et al. Characterization of a Sarcocystis neurona isolate from a Missouri horse with equine protozoal myeloencephalitis. Vet Parasitol 95 (2001) 143-154