Enzyme-linked immunosorbent assays for detection of equine antibodies specific to Sarcocystis neurona surface antigens

Clinical and Diagnostic Laboratory Immunology

Volumn 12, Issue 9, 2005, Pages 1050-1056

  Hoane, Jessica S ^a   Morrow, Jennifer K ^b   Saville, William J ^c   Dubey, J P ^d   Granstrom, David E ^d   Howe, Daniel K ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

^b Equine Biodiagnostics/IDEXX   (United States)

^c OHIO STATE UNIVERSITY   (United States)

^d AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; MEMBRANE ANTIGEN; PARASITE ANTIGEN; RECOMBINANT ANTIGEN;

ACCURACY; ANTIBODY DETECTION; ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ARTICLE; CONTROLLED STUDY; CROSS REACTION; DIAGNOSTIC TEST; ENCEPHALOMYELITIS; ENZYME LINKED IMMUNOSORBENT ASSAY; EQUINE PROTOZOAL MYELOENCEPHALITIS; HORSE DISEASE; HOST PARASITE INTERACTION; NEOSPORA; NEOSPORA HUGHESI; NONHUMAN; PRIORITY JOURNAL; PROTOZOAL INFECTION; RELIABILITY; SARCOCYSTIS; SARCOCYSTIS FAYERI; SARCOCYSTIS NEURONA; SEROLOGY; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, PROTOZOAN; ANTIBODY SPECIFICITY; ANTIGENS, SURFACE; BLOTTING, WESTERN; CROSS REACTIONS; ENCEPHALOMYELITIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HORSE DISEASES; HORSES; RECOMBINANT PROTEINS; SARCOCYSTIS; SARCOCYSTOSIS; SENSITIVITY AND SPECIFICITY;

EID: 24944435396     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/CDLI.12.9.1050-1056.2005     Document Type: Article

References (41)

1. Bentz, B. G., K. A. Ealey, J. Morrow, P. L. Claypool, and J. T. Saliki. 2003. Seroprevalence of antibodies to Sarcocystis neurona in equids residing in Oklahoma. J. Vet. Diagn. Investig. 15:597-600.
2. Bentz, B. G., D. E. Granstrom, and S. Stamper. 1997. Seroprevalence of antibodies to Sarcocystis neurona in horses residing in a county of southeastern Pennsylvania. J. Am. Vet. Med. Assoc. 210:517-518.
3. Blythe, L. L., D. E. Granstrom, D. E. Hansen, L. L. Walker, J. Bartlett, and S. Stamper. 1997. Seroprevalence of antibodies to Sarcocystis neurona in horses residing in Oregon. J. Am. Vet. Med. Assoc. 210:525-527.
4. Chahan, B., I. Gaturaga, X. Huang, M. Liao, S. Fukumoto, H. Hirata, Y. Nishikawa, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, I. Igarashi, T. Mikami, and X. Xuan. 2003. Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1. Vet. Parasitol. 118:177-185.
5. Duarte, P. C., B. M. Daft, P. A. Conrad, A. E. Packham, and I. A. Gardner. 2003. Comparison of serum indirect fluorescent antibody test with two Western blot tests for the diagnosis of equine protozoal myeloencephalitis. J. Vet. Diagn. Investig. 15:8-13.
6. Dubey, J. P., S. W. Davis, C. A. Speer, D. D. Bowman, A. de Lahunta, D. E. Granstrom, M. J. Topper, A. N. Hamir, J. F. Cummings, and M. M. Suter. 1991. Sarcocystis neurona n. sp. (Protozoa: Apicomplexa), the etiologic agent of equine protozoal myeloencephalitis. J. Parasitol. 77:212-218.
7. Dubey, J. P., S. Liddell, D. Mattson, C. A. Speer, D. K. Howe, and M. C. Jenkins. 2001. Characterization of the Oregon isolate of Neospora hughesi from a horse. J. Parasitol. 87:345-353.
8. Dubey, J. P., and D. S. Lindsay. 1998. Isolation in immunodeficient mice of Sarcocystis neurona from opossum (Didelphis virginiana) faeces, and its differentiation from Sarcocystis falcatula. Int. J. Parasitol. 28:1823-1828.
9. Dubey, J. P., D. S, Lindsay, W. J. Saville, S. M. Reed, D. E. Granstrom, and C. A. Speer. 2001. A review of Sarcocystis neurona and equine protozoal myeloencephalitis (EPM). Vet. Parasitol. 95:89-131.
10. Dubey, J. P., S. M. Mitchell, J. K. Morrow, J. C. Rhyan, L. M. Stewart, D. E. Granstrom, S. Romand, P. Thulliez, W. J. Saville, and D. S. Lindsay. 2003. Prevalence of antibodies to Neospora caninum, Sarcocystis neurona, and Toxoplasma gondii in wild horses from central Wyoming. J. Parasitol. 89: 716-720.
11. Dubey, J. P., R. H. Streitel, P. C. Stromberg, and M. J. Toussant. 1977. Sarcocystis fayeri sp. n. from the horse. J. Parasitol. 63:443-447.
12. Ellison, S. P., T. J. Kennedy, and K. K. Brown. 2003. Development of an ELISA to detect antibodies to rSAG1 in the horse. Int. J. App. Res. Vet. Med. 1:318-327.
13. Ellison, S. P., A. L. Omara-Opyene, C. A. Yowell, A. E. Marsh, and J. B. Dame. 2002. Molecular characterisation of a major 29 kDa surface antigen of Sarcocystis neurona. Int. J. Parasitol. 32:217-225.
14. Fenger, C. K., D. E. Granstrom, A. A. Gajadhar, N. M. Williams, S. A. McCrillis, S. Stamper, J. L. Langemeier, and J. P. Dubey. 1997. Experimental induction of equine protozoal myeloencephalitis in horses using Sarcocystis sp. sporocysts from the opossum (Didelphis virginiana). Vet. Parasitol. 68:199-213.
15. Fenger, C. K., D. E. Granstrom, J. L. Langemeier, S. Stamper, J. M. Donahue, J. S. Patterson, A. A. Gajadhar, J. V. Marteniuk, Z. Xiaomin, and J. P. Dubey. 1995. Identification of opossums (Didelphis virginiana) as the putative definitive host of Sarcocystis neurona. J. Parasitol. 81:916-919.
16. Furr, M., R. MacKay, D. Granstrom, H. Schott II, and F. Andrews. 2002. Clinical diagnosis of equine protozoal myeloencephalitis (EPM). J. Vet. Intern. Med. 16:618-621.
17. Granstrom, D. E., J. P. Dubey, S. W. Davis, R. Payer, J. C. Fox, K. B. Poonacha, R. C. Giles, and P. F. Comer. 1993. Equine protozoal myeloencephalitis: antigen analysis of cultured Sarcocystis neurona merozoites. J. Vet. Diagn. Investig. 5:88-90.
18. Hamir, A. N., S. J. Tornquist, T. C. Gerros, M. J. Topper, and J. P. Dubey. 1998. Neospora caninum-associated equine protozoal mycloencephalitis. Vet. Parasitol. 79:269-274.
19. Hoane, J. S., M. R. Yeargan, S. Stamper, W. J. A. Saville, J. K. Morrow, D. S. Lindsay, and D. K. Howe. 2005. Recombinant NhSAG1 ELISA: a sensitive and specific assay for detecting antibodies against Neospora hughesi in equine serum. J. Parasitol. 91:446-452.
20. Howe, D. K., R. Y. Gaji, M. Mroz-Barrett, M. J. Gubbels, B. Striepen, and S. Stamper. 2005. Sarcocystis neurona merozoites express a family of immunogenic surface antigens that are orthologues of the Toxoplasma gondii surface antigens (SAGs) and SAG-related sequences. Infect. Immun. 73: 1023-1033.
21. Howe, D. K., K. Tang, P. A. Conrad, K. Sverlow, J. P. Dubey, and L. D. Sibley. 2002. Sensitive and specific identification of Neospora caninum infection of cattle based on detection of serum antibodies to recombinant Ncp29. Clin. Diagn. Lab. Immunol. 9:611-615.
22. Hyun, C., G. D. Gupta, and A. E. Marsh. 2003. Sequence comparison of Sarcocystis neurona surface antigen from multiple isolates. Vet. Parasitol. 112:11-20.
23. Kimbita, E. N., X. Xuan, X. Huang, T. Miyazawa, S. Fukumoto, M. Mishima, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, N. Suzuki, T. Mikami, and I. Igarashi. 2001. Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1. Vet. Parasitol. 102:35-44.
24. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
25. Lindsay, D. S., and J. P. Dubey. 2001. Direct agglutination test for the detection of antibodies to Sarcocystis neurona in experimentally infected animals. Vet. Parasitol. 95:179-186.
26. Louie, K., K. W. Sverlow, B. C. Barr, M. L. Anderson, and P. A. Conrad. 1997. Cloning and characterization of two recombinant Neospora protein fragments and their use in serodiagnosis of bovine neosporosis. Clin. Diagn. Lab. Immunol. 4:692-699.
27. MacKay, R. J. 1997. Equine protozoal myeloencephalitis. Vet. Clin. N. Am. Equine Pract. 13:79-96.
28. MacKay, R. J., D. E. Granstrom, W. J. Saville, and S. M. Reed. 2000. Equine protozoal myeloencephalitis. Vet. Clin. N. Am. Equine Pract. 16:405-425.
29. Marsh, A. E., B. C. Barr, J. Madigan, J. Lakritz, R. Nordhausen, and P. A. Conrad. 1996. Neosporosis as a cause of equine protozoal myeloencephalitis. J. Am. Vet. Med. Assoc. 209:1907-1913.
30. Marsh, A. E., B. C. Barr, A. E. Packham, and P. A. Conrad. 1998. Description of a new Neospora species (Protozoa: Apicomplexa: Sarcocystidae). J. Parasitol. 84:983-991.
31. Marsh, A. E., P. J. Johnson, J. Ramos-Vara, and G. C. Johnson. 2001. Characterization of a Sarcocystis neurona isolate from a Missouri horse with equine protozoal myeloencephalitis. Vet. Parasitol. 95:143-154.
32. Morley, P. S., and W. J. A. Saville. 1997. Equine protozoal myeloencephalitis: what does a positive test mean? Am. Assoc. Equine Pract. Ann. Proc. 43:1-5.
33. Nishikawa, Y., Y. Kousaka, K. Tragoolpua, X. Xuan, L. Makala, K. Fujisaki, T. Mikami, and H. Nagasawa. 2001. Characterization of Neospora caninum surface protein NcSRS2 based on baculovirus expression system and its application for serodiagnosis of Neospora infection. J. Clin. Microbiol. 39: 3987-3991.
34. Rossano, M. G., J. B. Kaneene, J. V. Marteniuk, B. D. Banks, H. C. Schott, and L. S. Mansfield, 2001. The seroprevalence of antibodies to Sarcocystis neurona in Michigan equids. Prev. Vet. Med. 48:113-128.
35. Rossano, M. G., L. S. Mansfield, J. B. Kaneene, A. J. Murphy, C. M. Brown, H. C. Schott II, and J. C. Fox. 2000. Improvement of Western blot test specificity for detecting equine serum antibodies to Sarcocystis neurona. J. Vet. Diagn. Investig. 12:28-32.
36. Santoro, F., D. Afchain, R. Pierce, J. Y. Cesbron, G. Ovalaque, and A. Capron. 1985. Serodiagnosis of Toxoplasma infection using a purified parasite protein (p30). Clin. Exp. Immunol. 62:262-269.
37. Saville, W. J., S. M. Reed, D. E. Granstrom, K. W. Hinchcliff, C. W. Kohn, T. E. Wittum, and S. Stamper. 1997. Seroprevalence of antibodies to Sarcocystis neurona in horses residing in Ohio. J. Am. Vet. Med. Assoc. 210: 519-524.
38. Saville, W. J. A., J. P. Dubey, M. J. Oglesbee, C. D. Sofaly, A. E. Marsh, E. Elitsur, M. C. Vianna, D. S. Lindsay, and S. M. Reed. 2004. Experimental infection of ponies with Sarcocystis fayeri and differentiation from Sarcocystis neurona infections in horses. J. Parasitol. 90:1487-1491.
39. Tillotson, K., P. M. McCue, D. E. Granstrom, D. A. Dargatz, M. O. Smith, and J. L. Traub-Dargatz. 1999. Seroprevalence of antibodies to Sarcocystis neurona in horses residing in northern Colorado. J. Equine Vet. Sci. 19:122-126.
40. Vardeleon, D., A. E. Marsh, J. G. Thorne, W. Loch, R. Young, and P. J. Johnson. 2001. Prevalence of Neospora hughesi and Sarcocystis neurona antibodies in horses from various geographical locations. Vet. Parasitol. 95: 273-282.
41. Wright, P. F., E. Nilsson, E. M. VanRooij, M. Lelenta, and M. H. Jeggo. 1993. Standardisation and validation of enzyme-linked immunosorbent assay techniques for the detection of antibody in infectious disease diagnosis. Rev. Sci. Technol. 12:435-450.