Absolute quantification of farnesylated Ras levels in complex samples using liquid chromatography fractionation combined with tryptic digestion and electrospray tandem mass spectrometry

Analytical Biochemistry

Volumn 352, Issue 1, 2006, Pages 33-40

  Appels, Natalie M G M ^a   Rosing, Hilde ^a   Stephens, Trevor C ^b   Hughes, Andrew ^b   Schellens, Jan H M ^a,c   Beijnen, Jos H ^a,b,c  

^a NETHERLANDS CANCER INSTITUTE   (Netherlands)

^b ASTRAZENECA   (United Kingdom)

^c Science Faculty   (Netherlands)

Author keywords

Absolute quantification; Farnesylation; FTI; Mass spectrometry; Ras

Indexed keywords

AMINO ACIDS; MASS SPECTROMETRY; PEPTIDES; RADIUM;

ABSOLUTE QUANTIFICATION; DOSE-EFFECT RELATIONSHIPS; ELECTROSPRAY TANDEM MASS SPECTROMETRY; FARNESYL TRANSFERASE INHIBITORS; FARNESYLATION; LOWER LIMIT OF QUANTIFICATIONS; POST-TRANSLATIONAL MODIFICATIONS; REVERSED PHASE LIQUID-CHROMATOGRAPHY;

LIQUID CHROMATOGRAPHY;

CYSTEINE; LEUCINE; PROTEIN FARNESYLTRANSFERASE INHIBITOR; RAS PROTEIN; SERINE; TRYPSIN; VALINE;

ARTICLE; CELL LYSATE; DOSE RESPONSE; ELECTROSPRAY; FARNESYLATION; HUMAN; HUMAN CELL; ONCOGENE H RAS; PRIORITY JOURNAL; PROTEIN MODIFICATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; STANDARD; TANDEM MASS SPECTROMETRY; TECHNIQUE; TUMOR CELL;

EID: 33646061668     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.02.028     Document Type: Article

References (16)

1. Kato K., Cox A.D., Hisaka M.M., Graham S.M., Buss J.E., and Der C.J. Isoprenoid addition to Ras protein is the critical modification for its membrane association and transforming activity. Proc. Natl. Acad. Sci. USA 89 (1992) 6403-6407
2. Whyte D.B., Kirschmeier P., Hockenberry T.N., Nunez-Oliva I., James L., Catino J.J., Bishop W.R., and Pai J.K. K- and N-Ras are geranylgeranylated in cells treated with farnesyl protein transferase inhibitors. J. Biol. Chem. 272 (1997) 14459-14464
3. Kohl N.E., Conner M.W., Gibbs J.B., Graham S.L., Hartman G.D., and Oliff A. Development of inhibitors of protein farnesylation as potential chemotherapeutic agents. J. Cell Biochem. Suppl. 22 (1995) 145-150
4. Lerner E.C., Qian Y., Blaskovich M.A., Fossum R.D., Vogt A., Sun J., Cox A.D., Der C.J., Hamilton A.D., and Sebti S.M. Ras CAAX peptidomimetic FTI-277 selectively blocks oncogenic Ras signaling by inducing cytoplasmic accumulation of inactive Ras-Raf complexes. J. Biol. Chem. 270 (1995) 26802-26806
5. James G., Goldstein J.L., and Brown M.S. Resistance of K-RasBV12 proteins to farnesyltransferase inhibitors in Rat1 cells. Proc. Natl. Acad. Sci. USA 93 (1996) 4454-4458
6. Desiderio D.M., and Zhu X. Quantitative analysis of methionine enkephalin and β-endorphin in the pituitary by liquid secondary ion mass spectrometry and tandem mass spectrometry. J. Chromatogr. A 794 (1998) 85-96
7. Sinnaeve B.A., and Van Bocxlaer J.F. Evaluation of nano-liquid chromatography-tandem mass spectrometry in a column switching setup for the absolute quantification of peptides in the picomolar range. J. Chromatogr. A 1058 (2004) 113-119
8. Stokvis E., Rosing H., Lopez-Lazaro L., Rodriguez I., Jimeno J.M., Supko J.G., Schellens J.H., and Beijnen J.H. Quantitative analysis of the novel depsipeptide anticancer drug Kahalalide F in human plasma by high-performance liquid chromatography under basic conditions coupled to electrospray ionization tandem mass spectrometry. J. Mass Spectrom. 37 (2002) 992-1000
9. Yang J.Z., Bastian K.C., Moore R.D., Stobaugh J.F., and Borchardt R.T. Quantitative analysis of a model opioid peptide and its cyclic prodrugs in rat plasma using high-performance liquid chromatography with fluorescence and tandem mass spectrometric detection. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 780 (2002) 269-281
10. Yin J., Aviles P., Lee W., Ly C., Guillen M.J., Calvo P., Manzanares I., and Faircloth G. Validation of a sensitive assay for thiocoraline in mouse plasma using liquid chromatography-tandem mass spectrometry. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 794 (2003) 89-98
11. Barnidge D.R., Dratz E.A., Martin T., Bonilla L.E., Moran L.B., and Lindall A. Absolute quantification of the G protein-coupled receptor rhodopsin by LC/MS/MS using proteolysis product peptides and synthetic peptide standards. Anal. Chem. 75 (2003) 445-451
12. Gerber S.A., Rush J., Stemman O., Kirschner M.W., and Gygi S.P. Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS. Proc. Natl. Acad. Sci. USA 100 (2003) 6940-6945
13. 13C-labeled peptide standards. Proteomics 4 (2004) 1175-1186
14. Zhang F., Bartels M.J., Brodeur J.C., and Woodburn K.B. Quantitative measurement of fathead minnow vitellogenin by liquid chromatography combined with tandem mass spectrometry using a signature peptide of vitellogenin. Environ. Toxicol. Chem. 23 (2004) 1408-1415
15. Miller K.J., Bowsher R.R., Celniker A., Gibbons J., Gupta S., Lee J.W., Swanson S.J., Smith W.C., and Weiner R.S. Workshop on bioanalytical methods validation for macromolecules: summary report. Pharm. Res. 18 (2001) 1373-1383
16. Buser C.A., Dinsmore C.J., Fernandes C., Greenberg I., Hamilton K., Mosser S.D., Walsh E.S., Williams T.M., and Koblan K.S. High-performance liquid chromatography/mass spectrometry characterization of Ki4B-Ras in PSN-1 cells treated with the prenyltransferase inhibitor L-778123. Anal. Biochem. 290 (2001) 126-137