Large-scale purification of recombinant human angiostatin

Protein Expression and Purification

Volumn 20, Issue 2, 2000, Pages 216-227

  Shepard, Scot R ^a   Boucher, Robert ^a   Johnston, Jeremy ^a   Boerner, Renee ^a   Koch, George ^a   Madsen, John W ^b   Grella, Davida ^b   Sim, B Kim Lee ^b   Schrimsher, Jeffrey L ^a  

^a Purification Proc Devmt Department   (United States)

^b ENTREMED INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; ANGIOSTATIN; FERMENTATION; HYDROPHOBICITY; PROTEIN PURIFICATION;

PICHIA PASTORIS;

EID: 0033777693     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1276     Document Type: Article

References (15)

1. Angiostatin: A novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma
2. Angiostatin induces and sustains dormancy of human primary tumors in mice
3. A recombinant human angiostatin protein inhibits experimental primary and metastatic cancer
4. Angiostatin and Endostatin: Endothelial cell-specific endogenous inhibitors of angiogenesis and tumor growth
5. Domains in human plasminogen
6. The primary structure of human plasminogen: Isolation of two lysine-binding fragments and one 'mini'-plasminogen (MW 38,000) by elastase-catalyzed-specific limited proteolysis
7. The tumor-suppressing activity of angiostatin protein resides within kringles 1 to 3
8. Kringle domains of human angiostatin
9. Pilot scale recovery of recombinant annexin V from unclarified E. coli homogenate using expanded bed adsorption
10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
11. Manufacture of recombinant proteins with safe and validated chromatographic sorbents
12. Interspecies cross-reactivity of monoclonal antibodies to various epitopes of human plasminogen