Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3)

Journal of Allergy and Clinical Immunology

Volumn 117, Issue 4, 2006, Pages 848-854

  Grunwald, Thomas ^a   Bockisch, Benjamin ^a   Spillner, Edzard ^b   Ring, Johannes ^c   Bredehorst, Reinhard ^b   Ollert, Markus W ^c  

^a Protein Ligand Structural PLS Design GmbH   (Germany)

^b UNIVERSITY OF HAMBURG   (Germany)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

acid phosphatase; Api m 3; Apis mellifera; expression; Honeybee venom; insect cells; recombinant allergen

Indexed keywords

ACID PHOSPHATASE; ALLERGEN; BEE VENOM; COMPLEMENTARY DNA; DNA FRAGMENT; IMMUNOGLOBULIN E ANTIBODY; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANTIGEN ANTIBODY REACTION; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST; ENZYME ACTIVITY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; IMMUNOREACTIVITY; IMMUNOTHERAPY; INSECT ALLERGY; INSECT CELL; MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS; WESTERN BLOTTING;

ACID PHOSPHATASE; ALLERGENS; AMINO ACID SEQUENCE; ANAPHYLAXIS; ANIMALS; BASE SEQUENCE; BEE VENOMS; BEES; CELL LINE; CLONING, MOLECULAR; DESENSITIZATION, IMMUNOLOGIC; DNA, COMPLEMENTARY; GENE EXPRESSION; GENES, INSECT; HUMANS; IMMUNOGLOBULIN E; INSECT BITES AND STINGS; MOLECULAR SEQUENCE DATA; MOTHS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 33646023665     PISSN: 00916749     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jaci.2005.12.1331     Document Type: Article

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