Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution

Biotechnology and Bioengineering

Volumn 93, Issue 5, 2006, Pages 1023-1027

  Bakke, Mikio ^a   Setoyama, Chiaki ^b   Miura, Retsu ^b   Kajiyama, Naoki ^a  

^a KIKKOMAN CORPORATION   (Japan)

^b KUMAMOTO UNIVERSITY   (Japan)

Author keywords

Decreased Km value; Increased thermal stability; Porcine kidney D aminoacid oxidase; Random mutagenesis

Indexed keywords

AMINO ACIDS; BIOASSAY; BIOLOGICAL ORGANS; ENZYMES; MUTAGENESIS; SUBSTRATES;

DECREASED KM VALUE; INCREASED THERMAL STABILITY; PORCINE KIDNEY D-AMINOACID OXIDASE; RANDOM MUTAGENESIS;

ENZYME KINETICS;

DEXTRO AMINO ACID OXIDASE;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVITY; KIDNEY; MUTAGENESIS; NONHUMAN; SWINE; THERMOSTABILITY;

AMINO ACID SUBSTITUTION; ANIMALS; BINDING SITES; D-AMINO-ACID OXIDASE; DIMERIZATION; ENZYME STABILITY; ESCHERICHIA COLI; GENETIC VECTORS; HEAT; HYDROGEN-ION CONCENTRATION; KIDNEY; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SWINE; TEMPERATURE; TRANSFECTION;

SUIDAE; SUS SCROFA;

EID: 33646020382     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.20754     Document Type: Article

References (22)

1. Bakke M, Kajiyama N. 2004. Improvement in thermal stability and substrate binding of pig kidney D-amino acid oxidase by chemical modification. Appl Biochem Biotechnol 112:123-131.
2. Betancor L, Hidalgo A, Fernandez-Lorente G, Mateo C, Rodriguez V, Fuentes M, Lopez-Gallego F, Fernandez-Lafuente R, Guisan JM. 2003. Use of physicochemical tools to determine the choice of optimal enzyme: Stabilization of D-amino acid oxidase. Biotechnol Prog 19:784-788.
3. Cadwell RC, Joyce GF. 1994. Mutagenic PCR. PCR Methods Appl 3:S136-S140.
4. Curti B, Ronchi S, Branzoli U, Ferri G, Williams CH Jr. 1973. Improved purification, amino acid analysis and molecular weight of homogenous D-amino acid oxidase from pig kidney. Biochim Biophys Acta 327:266-273.
5. Friedman M. 1999. Chemistry, nutrition, and microbiology of D-amino acids. J Agric Food Chem 47:3457-3479.
6. Fukui K, Watanabe F, Shibata T, Miyake Y. 1987. Molecular cloning and sequence of cDNAs encoding porcine kidney D-amino acid oxidase. Biochemistry 26:3612-3618.
7. Gandolfi I, Palla G, Delprato L, De Nisco F, Marchelli R, Salvadori C. 1992. D-Amino acid in milk as related to heat treatments and bacterial activity. J Food Sci 57:377-379.
8. Hanson RL, Schwinden MD, Banerjee A, Brzozowski DB, Chen BC, Patel BP, McNamee CG, Kodersha GA, Kronenthal DR, Patel RN, Szarka LJ. 1999. Enzymatic synthesis of L-6-hydroxynorleucine. Bioorg Med Chem 7:2247-2252.
9. Inaba Y, Mizukami K, Hamada-Sato N, Kobayashi T, Imada C, Watanabe E. 2003. Development of a D-alanine sensor for the monitoring of a fermentation using the improved selectivity by the combination of D-amino acid oxidase and pyruvate oxidase. Biosens Bioelectron 19:423-431.
10. Man EH, Fisher GH, Payan IL, Cadilla-Perezrios R, Garcia NM, Chemburkar R. 1987. D-Aspartate in human brain. J Neurochem 48 II :510-515.
11. Mateo C, Abian O, Fernandez-Lafuente R, Guisan JM. 2000. Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support-polyethylenimine composites. Biotechnol Bioeng 68:98-105.
12. Mattevi A, Vanoni MA, Todone F, Rizzi M, Teplyakov A, Coda A, Bologesi M, Curti B. 1996. Crystal structure of D-amino acid oxidase: A case of active site mirror-image convergent evolution with flavocytochrome b2. Proc Natl Acad Sci USA 93:7496-7501.
13. Mazzeo P, Romeo A. 1972. Enzymic and chemical transformations of the side chain of cephalosporin C. J Chem Soc Perkin 120:2532.
14. Miura R, Setoyama C, Nishina Y, Shiga K, Mizutani H, Miyahara I, Hirotsu K. 1997. Structural and mechanistic studies on D-amino acid oxidaseñ substrate complex: Implications of the crystal structure of enzymeñ substrate analog complex. J Biochem 122:825-833.
15. Miyake Y, Abe T, Yamano T. 1971. Substrate induced dimerization of D-amino-acid oxidase. J Biochem 70:719-722.
16. Mizutani H, Miyahara I, Hirotsu K, Nishina Y, Shiga K, Setoyama C, Miura R. 1996. Three-dimensional structure of porcine kidney D-amino acid oxidase at 3.0 Å resolution. J Biochem 120:14-17.
17. Nagata Y, Akino T, Ohno K, Kataoka Y, Ueda T, Sakurai T, Shiroshita K, Yasuda T. 1987. Free D-amino acids in human plasma in relation to senescence and renal diseases. Clin Sci 73:105-108.
18. Nohara Y, Suzuki J, Kinoshita T, Watanabe M. 2002. Creatinine inhibits D-amino acid oxidase. Nephron 91:281-285.
19. Pilone MS. 2000. D-amino acid oxidase: New findings. Cell Mol Life Sci 57:1732-1747.
20. Poinar HN, Hoss M, Bada JL, Paabo S. 1999. Amino acid racemization and preservation of ancient DNA. Science 272:864-866.
21. Roff GJ, Lloyd RC, Turner NJ. 2004. A versatile chemo-enzymatic route to enantiomerically pure β-branched α-amino acids. J Am Chem Soc 126:4098-4099.
22. Setoyama C, Miura R, Nishina Y, Shiga K, Mizutani H, Miyahara I, Hirotsu K. 1996. Crystallization of expressed porcine kidney D-amino acid oxidase and preliminary X-ray crystallographic characterization. J Biochem 119:1114-1117.