Green fluorescent chimeras indicate nonpolar localization of pullulanase secreton components PulL and PulM

Journal of Bacteriology

Volumn 188, Issue 8, 2006, Pages 2928-2935

  Buddelmeijer, Nienke ^a   Francetic, Olivera ^a   Pugsley, Anthony P ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CHIMERIC PROTEIN; GREEN FLUORESCENT PROTEIN; PULL PROTEIN; PULLULANASE; PULM PROTEIN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL MEMBRANE; CELL SURFACE; CELLULAR DISTRIBUTION; CHEMICAL LABELING; ENZYME LOCALIZATION; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; FRACTIONATION; KLEBSIELLA OXYTOCA; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN SECRETION; SECRETON; VIBRIO CHOLERAE;

ARTIFICIAL GENE FUSION; BACTERIAL PROTEINS; CELL FRACTIONATION; ESCHERICHIA COLI; GENES, REPORTER; GLYCOSIDE HYDROLASES; GREEN FLUORESCENT PROTEINS; KLEBSIELLA OXYTOCA; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; PROTEIN TRANSPORT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; KLEBSIELLA OXYTOCA; VIBRIO; VIBRIO CHOLERAE;

EID: 33646009982     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.188.8.2928-2935.2006     Document Type: Article

References (53)

1. Ball, G., V. Chapon-Hervé, S. Bleves, G. Michel, and M. Bally. 1999. Assembly of XcpR in the cytoplasmic membrane is required for extracellular protein secretion in Pseudomonas aeruginosa. J. Bacteriol. 181:382-388.
2. Bleves, S., M. Gérard-Vincent, A. Lazdunski, and A. Filloux. 1999. Structure-function analysis of XcpP, a component involved in general secretory pathway-dependent protein secretion in Pseudomonas aentginosa. J. Bacteriol. 181:4012-4019.
3. Boyd, D., D. S. Weiss, J. C. Chen, and J. Beckwith. 2000. Towards single-copy gene expression systems making gene cloning physiologically relevant: lambda InCh, a simple Escherichia coli plasmid-chromosome shuttle system. J. Bacteriol. 182:842-847.
4. Brandon, L. D., N. Goehring, A. Janakiraman, A. W. Yan, T. Wu, J. Beck-with, and M. B. Goldberg. 2003. IcsA, a polarly localized autotransporter with an atypical signal peptide, uses the Sec apparatus for secretion, although the Sec apparatus is circumferentially distributed. Mol. Microbiol. 50:45-60.
5. Camberg, J., and M. Sandkvist. 2005. Molecular analysis of the Vibrio choleras type II secretion ATPase EpsE. J. Bacteriol. 187:249-256.
6. Campo, N., H. Tjalsma, G. Buist, D. Stepniak, M. Meijer, M. Veenhuis, M. Westermann, J. P. Müller, S. Bron, J. Kok, O. P. Kuipers, and J. D. H. Jongbloed. 2004. Subcellular sites for bacterial protein export. Mol. Microbiol. 53:1583-1599.
7. Chami, M., I. Guilvout, M. Gregorini, H. Remigy, S. Muller, M. Valerio, A. Engel, A. P. Pugsley, and N. Bayan. 2005. Structural insights into the secretin PulD and its trypsin resistant core. J. Biol. Chem. 280:37732-37741.
8. Chang, H.-C., C. M. Kaiser, U. Hartle, and J. M. Barral. 2005. De novo folding of GFP fusion proteins: high efficiency in eukaryotes but not in bacteria. J. Mol. Biol. 353:397-409.
9. Chiang, P., M. Habash, and L. L. Burrows. 2005. Disparate subcellular localization patterns of Pseudomonas aeruginosa type IV pilus ATPases involved in twitching motility. J. Bacteriol. 187:829-839.
10. d'Enfert, C., A. Ryter, and A. P. Pugsley. 1987. Cloning and expression in Escherichia coli of the Klebsiella pneumoniae genes for production, surface localization and secretion of the lipoprotein pullulanase. EMBO J. 6:3531-3538.
11. Durand, E., A. Bernadac, G. Ball, A. Lazdunski, J. N. Sturgis, and A. Filloux. 2003. Type II protein secretion in Pseudomonas aeruginosa: the pseudopilus is a multifibrillar and adhesive structure. J. Bacteriol. 185:2749-2758.
12. Durand, E., G. Michel, R. Voulhoux, J. Kurner, A. Bernadac, and A. Filloux. 2005. XcpX controls biogenesis of the Pseudomonas aeruginosa XcpT-containing pseudopilus. J. Biol. Chem. 280:31378-31389.
13. Filloux, A. 2004. The underlying mechanism of type II protein secretion. Biochim. Biophys. Acta 1694:163-179.
14. Francetic, O., D. Belin, C. Badaut, and A. P. Pugsley. 2000. Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion. EMBO. J. 19:6697-6703.
15. Ghosh, A. S., and K. D. Young. 2005. Helical disposition of proteins and lipopolysaccharide in the outer membrane of Escherichia coli. J. Bacteriol. 187:1913-1922.
16. Gibbs, K. A., D. D. Isaac, J. Xu, R. W. Hendrix, T. J. Silhavy, and J. A. Theriot. 2004. Complex spatial distribution and dynamics of an abundant Escherichia coli outer membrane protein, LamB. Mol. Microbiol. 53:1771-1783.
17. Hardie, K. R., S. Lory, and A. P. Pugsley. 1996. Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like protein. EMBO J. 15:978-988.
18. Judd, P. K., R. B. Kumar, and A. Das. 2005. Spatial location and requirements for the assembly of the Agrobacterium tumefaciens type IV secretion apparatus. Proc. Natl. Acad. Sci. USA 102:11498-11503.
19. Judd, P. K., R. B. Kumar, and A. Das. 2005. The type IV secretion apparatus protein VirB6 of Agrobacterium tumefaciens localizes to a cell pole. Mol. Microbiol. 55:115-124.
20. Köhler, R., K. Schäfer, S. Müller, G. Vignon, K. Driedrichs, A. Philippsen, P. Ringler, A. P. Pugsley, A. Engel, and W. Weite. 2004. Structure and assembly of pseudopilin PulG. Mol. Microbiol. 54:647-664.
21. Kornacker, M. G., and A. P. Pugsley. 1989. Molecular characterization of pulA and its product, pullulanase, a secreted enzyme of Klebsiella pneumoniae UNF5023. Mol. Microbiol. 4:73-85.
22. Kruse, T., and K. Gerdes. 2005. Bacterial DNA segregation by the actin-like MreB protein. Trends Cell Biol. 15:343-345.
23. Kumar, R. B., X. Yong-Hong, and A. Das. 2000. Subcellular localization of the Agrobacterium tumefaciens T-DNA transport pore proteins: VirB is essential for the assembly of the transport pore. Mol. Microbiol. 36:608-617.
24. Letellier, L., S. P. Howard, and T. J. Buckley. 1997. Studies on the energetics of proaerolysin secretion across of the outer membrane of Aeromonas spp: evidence for requirement for both the protonmotive force and ATP. J. Biol. Chem. 272:11109-11113.
25. Maddock, J., and L. Shapiro. 1993. Polar location of the chemoreceptor complex in the Escherichia coli cell. Science 259:1717-1723.
26. Michel, G., S. Bleves, G. Ball, A. Lazdunski, and A. Filloux. 1998. Mutual stabilization of the XcpZ and XcpY components of the secretory apparatus in Pseudomonas aeruginosa. Microbiology 144:3379-3386.
27. Miller, J. H. 1992. A short course in bacterial genetics. A laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
28. Nilsen, T., A. W. Yan, G. Gale, and M. B. Goldberg. 2005. Presence of multiple sites containing polar material in spherical Escherichia coli cells that lack MreB. J. Bacteriol. 187:6187-6196.
29. Nunn, D., and S. Lory. 1992. Components of the protein excretion apparatus of Pseudomonas aeruginosa are processed by the tvpe IV prepilin peptidase. Proc. Natl. Acad. Sci. USA 89:47-51.
30. Peabody, C. R., Y. C. Chung, M. R. Yen, D. Vidal-Ingigliardi, A. P. Pugsley, and M. H. Saier, Jr. 2003. Type II protein secretion and its relationship to bacterial type IV pili and archaeal flagella. Microbiology 149:3051-3072.
31. Possot, O., L. Letellier, and A. P. Pugsley. 1997. Energy requirement for pullulanase secretion by the main terminal branch of the general secretory pathway. Mol. Microbiol. 24:457-464.
32. Possot, O., G. Vignon, N. Bomchil, F. Ebel, and A. P. Pugsley. 2000. Multiple interactions between pullulanase secreton components involved in stabilization and cytoplasmic membrane association of PulE. J. Bacteriol. 182:2142-2152.
33. Possot, O. M., M. Gérard-Vincent, and A. P. Pugsley. 1999. Membrane association and multimerization of secreton component PulC. J. Bacteriol. 181:4004-4011.
34. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
35. Pugsley, A. P., and B. Dupuy. 1992. An enzyme with type IV prepilin peptidase activity is required to process a component of the general extracellular protein secretion pathway of Klebsiella oxytoca. Mol. Microbiol. 6:751-760.
36. Pugsley, A. P., M. G. Kornacker, and I. Poquet. 1991. The general secretion pathway is directly required for extracellular pullulanase secretion in Escherichia coli. Mol. Microbiol. 5:343-352.
37. Pugsley, A. P., M. G. Kornacker, and A. Ryter. 1990. Analysis of the subcellular location of pullulanase produced by Escherichia coli carrying the pulA gene from Klebsiella pneumoniae strain UNF5023. Mol. Microbiol. 4:59-72.
38. Pugsley, A. P., and I. Reyss. 1990. Five genes at the 3′ end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion. Mol. Microbiol. 4:365-379.
39. Py, B., L. Loiseau, and F. Barras. 1999. Assembly of the type II secretion machinery of Erwinia chrysanthemi: direct interaction and associated conformational change between OutE, the putative ATP-binding component and the membrane protein OutL. J. Mol. Biol. 289:659-670.
40. Py, B., L. Loiseau, and F. Barras. 2001. An inner membrane platform in the type II secretion machinery of gram-negative bacteria. EMBO Rep. 2:244-248.
41. Ray, N., A. Nenninger, C. W. Mullineaux, and C. Robinson. 2005. Location and mobility of twin arginine translocase subunits in the Escherichia coli plasma membrane. J. Biol. Chem. 280:17961-17968.
42. Sandkvist, M. 2001. Type II secretion and pathogenesis. Infect. Immun. 69:3523-3535.
43. Sandkvist, M., M. Bagdasarian, S. P. Howard, and V. J. DiRita. 1995. Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae. EMBO. J. 14:1664-1673.
44. Sandkvist, M., L. P. Hough, M. M. Bagdasarian, and M. Bagdasarian. 1999. Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio cholerae. J. Bacteriol. 181:3129-3135.
45. Sandkvist, M., J. M. Keith, M. Bagdasarian, and S. P. Howard. 2000. Two regions of EpsL involved in species-specific protein-protein interactions with EpsE and EpsM of the general secretion pathway in Vibrio cholerae. J. Bacteriol. 182:742-748.
46. Sauvonnet, N., G. Vignon, A. P. Pugsley, and P. Gounon. 2000. Pilus formation and protein secretion by the same machinery in Escherichia coli. EMBO J. 19:2221-2228.
47. Scott, M., Z. Dossani, and M. Sandkvist. 2001. Directed polar secretion of protease from single cells of Vibrio cholerae via the type II secretion pathway. Proc. Natl. Acad. Sci. USA 98:13978-13983.
48. Skerker, J. M., and H. C. Berg. 2001. Direct observation of extension and retraction of type IV pili. Proc. Natl. Acad. Sci. USA 98:6901-6904.
49. Takeshita, S., M. Sato, M. Toba, W. Masahashi, and T. Hashimoto-Goto. 1987. High copy number and low copy number plasmid vectors for lacZ α-complementation and chloramphenicol- or kanamycin-resistance selection. Gene 61:63-74.
50. Vignon, G., R. Köhler, E. Larquet, S. Giroux, M.-C. Prévost, P. Roux, and A. P. Pugsley. 2003. Type IV-like pili formed by the type II secreton: specificity, composition, bundling, polar localization and surface presentation of peptides. J. Bacteriol. 185:3416-3428.
51. Voulhoux, R., G. Ball, B. Ize, M. L. Vasil, A. Lazdunski, L.-F. Wu, and A. Filloux. 2001. Involvement of the twin-arginine translocation system in protein secretion via the type It pathway. EMBO. J. 20:6735-6741.
52. Weiss, D. S. 2004. Bacterial cell division and the septal ring. Mol. Microbiol. 54:588-597.
53. Weiss, D. S., J. C. Chen, J. M. Ghigo, D. Boyd, and J. Beckwith. 1999. Localization of Ftsl (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL. J. Bacteriol. 181:508-520.