Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis

BioTechniques

Volumn 40, Issue 3, 2006, Pages 315-319

  Lind, Kristina ^a,b   Ståhlberg, Anders ^b,c   Zoric, Neven ^b   Kubista, Mikael ^a,b  

^a CHALMERS UNIVERSITY OF TECHNOLOGY   (Sweden)

^b TATAA Biocenter   (Sweden)

^c LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING DYES; LOCKED NUCLEIC ACID (LNA); MELTING CURVE ANALYSIS; PRIMER-DIMER FORMATION;

BINDING SITES; DIMERS; DNA; MELTING; REACTION KINETICS; REAL TIME SYSTEMS;

NUCLEIC ACIDS;

BOXTO; DIMER; DOUBLE STRANDED DNA; DYE; GENE PRODUCT; UNCLASSIFIED DRUG; DNA; FLUORESCENT DYE;

AGAR GEL ELECTROPHORESIS; ARTICLE; DIMERIZATION; DNA PROBE; DNA SEQUENCE; DNA TEMPLATE; FLUORESCENCE; GENE AMPLIFICATION; GENE TARGETING; MELTING POINT; NUCLEIC ACID AMPLIFICATION; REAL TIME POLYMERASE CHAIN REACTION; DNA DENATURATION; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; GENETICS; METHODOLOGY; POLYMERASE CHAIN REACTION; TRANSITION TEMPERATURE;

DNA; DNA PROBES; FLUORESCENT DYES; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, FLUORESCENCE; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TRANSITION TEMPERATURE;

EID: 33646005816     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112101     Document Type: Article

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