Treatment with vector-expressed small hairpin RNAs against Ki67 RNA-induced cell growth inhibition and apoptosis in human renal carcinoma cells

Acta Biochimica et Biophysica Sinica

Volumn 38, Issue 4, 2006, Pages 254-261

  Zheng, Jun Nian ^a,b   Sun, Ya Feng ^a   Pei, Dong Sheng ^a   Liu, Jun Jie ^a   Ma, Teng Xiang ^b   Han, Rui Fa ^b   Li, Wang ^a   Zheng, Dian Bao ^a   Chen, Jia Cun ^a   Sun, Xiao Qing ^a  

^a XUZHOU MEDICAL UNIVERSITY   (China)

^b TIANJIN MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Ki67 gene; Proliferation; shRNAs renal cell carcinoma

Indexed keywords

KI 67 ANTIGEN; MESSENGER RNA; SMALL INTERFERING RNA;

APOPTOSIS; ARTICLE; BIOSYNTHESIS; CELL PROLIFERATION; DRUG EFFECT; GENE VECTOR; GENETIC TRANSFECTION; HUMAN; KIDNEY TUMOR; NICK END LABELING; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR CELL LINE;

APOPTOSIS; CELL LINE, TUMOR; CELL PROLIFERATION; GENETIC VECTORS; HUMANS; IN SITU NICK-END LABELING; KI-67 ANTIGEN; KIDNEY NEOPLASMS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, SMALL INTERFERING; TRANSFECTION;

MAMMALIA;

EID: 33645969576     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1111/j.1745-7270.2006.00158.x     Document Type: Article

References (19)

1. MacCallum DE Hall PA. Biochemical characterization of pKi67 with the identification of a mitotic-specific form associated with hyperphosphorylation and altered DNA binding. Exp Cell Res 1999 252 186 198.
2. Ross W Hall PA. Ki67: From antibody to molecule to understanding Clin Mol Pathol 1995 48 M113 M117.
3. Menke TB Boettcher K Krüger S Kausch I Boehle A Sczakiel G Warnecke JM. Ki-67 protein concentrations in urothelial bladder carcinomas are related to Ki-67-specific RNA concentrations in urine. Clin Chem 2004 50 1461 1463.
4. Li R Heydon K Hammond ME Grignon DJ Roach M Wolkov HB Sandler HM. Ki-67 staining index predicts distant metastasis and survival in locally advanced prostate cancer treated with radiotherapy oncology group protocol 86-10. Clin Cancer Res 2004 10 4118 4124.
5. Endl E Gerdes J. Posttranslational modifications of the Ki-67 protein coincide with two major checkpoints during mitosis. J Cell Physiol 2000 182 371 380.
6. Endl E Gerdes J. The Ki-67 protein: Fascinating forms and an unknown function. Exp Cell Res 2000 257 231 237.
7. Schlüter C Duchrow M Wohlenberg C Becker MH Key G Flad HD Gerdes J. The cell proliferation-associated antigen of antibody Ki-67: Avery large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol 1993 123 513 522.
8. Kausch I Jiang H Ewerdwalbesloh N Doehn C Kruger S Sczakiel G Jocham D. Inhibition of Ki-67 in a renal cell carcinoma severe combined immunodeficiency disease mouse model is associated with induction of apoptosis and tumor growth inhibition. BJU Int 2005 95 416 420.
9. Zheng JN Sun YF Pei DS Liu JJ Sun XQ Chen JC Cai WQ. Anti-Ki-67 peptide nucleic acid affects the proliferation and apoptosis of human renal carcinoma cells in vitro. Life Sci 2005 76 1873 1881.
10. Zheng JN Ma TX Cao JY Sun XQ Chen JC Li W Wen RM. Knockdown of Ki-67 by small interfering RNA leads to inhibition of proliferation and induction of apoptosis in human renal carcinoma cells. Life Sci 2006 78 724 729.
11. Tuschl T. RNA interference and small interfering RNAs. Chembiochem 2001 2 239 245.
12. Yu JY DeRuiter SL Turner DL. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc Natl Acad Sci USA 2002 99 6047 6052.
13. Brummelkamp TR Bernards R Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science 2002 296 550 553.
14. Kobayashi N Matsui Y Kawase A Hirata K Miyagishi M Taira K Nishikawa M et al. Vector-based in vivo RNA interference: Dose- and time-dependent suppression of transgene expression. J Pharmacol Exp Ther 2004 308 688 693.
15. Lieberman J Song E Lee SK Shankar P. Interfering with disease: Opportunities and roadblocks to harnessing RNA interference. Trends Mol Med 2003 9 397 403.
16. Ou YC Gardner TA Kao C Zhau HE Chung LW. A potential for tissue restrictive gene therapy in renal cell carcinoma using MN/C AIX promoter. Anticancer Res 2005 25 881 886.
17. Coma S Noe V Lavarino C Adan J Rivas M Lopez-Matas M Pagan R et al. Use of siRNAs and antisense oligonucleotides against survivin RNA to inhibit steps leading to tumor angiogenesis. Oligonucleotides 2004 14 100 113.
18. Bertrand JR Pottier M Vekris A Opolon P Maksimenko A Malvy C. Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo. Biochem Biophys Res Commun 2002 296 1000 1004.
19. Sui G Soohoo C Affar ELB Gay F Shi Y Forrester WC Shi Y. ADNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci USA 2002 99 5515 5520.