Evaluation of the IDI-MRSA assay for detection of methicillin-resistant Staphylococcus aureus from nasal and rectal specimens pooled in a selective broth

Journal of Clinical Microbiology

Volumn 44, Issue 4, 2006, Pages 1219-1223

  Desjardins, M ^a,b   Guibord, Christiane ^b   Lalonde, B ^a   Toye, B ^a,b   Ramotar, K ^a,b  

^a UNIVERSITY OF OTTAWA   (Canada)

^b OTTAWA HOSPITAL RESEARCH INSTITUTE   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CEFOXITIN; OXACILLIN;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BROTH CULTURE; CULTURE MEDIUM; FALSE POSITIVE RESULT; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NOSE SWAB; PERFORMANCE; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; REAL TIME POLYMERASE CHAIN REACTION; RECTUM SWAB; SAMPLING; SENSITIVITY AND SPECIFICITY;

BACTERIAL TYPING TECHNIQUES; CULTURE MEDIA; HUMANS; METHICILLIN RESISTANCE; NOSE; REAGENT KITS, DIAGNOSTIC; RECTUM; SPECIMEN HANDLING; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

BACTERIA (MICROORGANISMS); METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS AUREUS;

EID: 33645899638     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.44.4.1219-1223.2006     Document Type: Article

References (31)

1. Alvarez, J., M. Sola, A. B. Vivanco, I. Perales, R. Cisterna, A. Rementeria, and J. Garaizar. 2004. Development of a multiplex PCR technique for detection and epidemiological typing of Salmonella in human clinical samples. J. Clin. Microbiol. 42:1734-1738.
2. Apfalter, P., O. Assadian, A. Kalczyk, V. Lindenmann, A. Makristathis, S. Mustafa, M. Rotter, and A. M. Hirschl. 2002. Performance of a new chromogenic oxacillin resistance screen medium (Oxoid) in the detection and presumptive identification of methicillin-resistant Staphylococcus aureus. Diagn. Microbiol. Infect. Dis. 44:209-211.
3. Blanc, D. S., A. Wenger, and J. Bille. 2003. Evaluation of a novel medium for screening specimens from hospitalized patients to detect methicillin-resistant Staphylococcus aureus. J. Clin. Microbiol. 41:3499-3502.
4. Canadian Communicable Disease Report. 2005. Surveillance for methicillin-resistant Staphylococcus aureus in Canadian hospitals-a report update from the Canadian Nosocomial Infection Surveillance Program. Can. Communicable Dis. Rep. 31:33-39.
5. Cosgrove, S. E., E. N. Sakoulas, E. N. Perencevich, M. J. Schwaber, and A. W. Karchmer. 2003. Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Can. Infect. Dis. 36:53-59.
6. Costa, A.-M., I. Kay, and S. Palladino. 2005. Rapid detection of mecA and nuc genes in staphylococci by real-time multiplex polymerase chain reaction. Diagn. Microbiol. Infect. Dis. 51:13-17.
7. Cuny, C., and W. Witte. 2005. PCR for the identification of methicillin-resistant Staphylococcus aureus (MRSA) strains using a single primer pair specific for SCCmec elements and the neighbouring chromosome-borne orfX. Clin. Microbiol. Infect. 11:834-837.
8. Donnio, P.-Y., D. C. Oliviera, N. A. Faria, N. Wilhelm, A. Le Coustumier, and H. de Lencastre. 2005. Partial excision of the chromosomal cassette containing the methicillin resistance determinant results in methicillin-susceptible Staphylococcus aureus. J. Clin. Microbiol. 43:4191-4193.
9. Fang, H., and G. Hedin. 2003. Rapid screening and identification of methicillin-resistant Staphylococcus aureus from clinical samples by selective-broth and real-time PCR assay. J. Clin. Microbiol. 41:2894-2899.
10. Francois, P., D. Pittet, M. Bento, B. Pepey, P. Vaudaux, D. Lew, and J. Schrenzel. 2003. Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay. J. Clin. Microbiol. 41:254-260.
11. Hiramatsu, K. 2004. Elucidation of the mechanism of antibiotic resistance acquisition of methicillin-resistant Staphylococcus aureus (MRSA) and determination of its whole genome nucleotide sequence. Jpn. Med. Assoc. J. 47:153-159.
12. Huletsky, A., R. Giroux, V. Rossbach, M. Gagnon, M. Vaillancourt, M. Bernier, F. Gagnon, K. Truchon, M. Bastien, F. J. Picard, A. van Belkum, M. Ouellette, P. H. Roy, and M. G. Bergeron. 2004. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J. Clin. Microbiol. 42:1875-1884.
13. Ito, T., Y. Katayama, K. Asada, N. Mori, K. Tsutsumimoto, C. Tiensasitorn, and K. Hiramatsu. 2001. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 45:1323-1336.
14. Katayama, Y., F. Takeuchi, T. Ito, X. X. Ma, Y. Ui-Mizutani, I. Kobayashi, and K. Hiramatsu. 2003. Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus. J. Bacteriol. 185:2711-2722.
15. Kim, T., P. I. Oh, and A. E. Simor. 2003. The economic impact of methicillin-resistant Staphylococcus aureus in Canadian hospitals. Infect. Control Hosp. Epidemiol. 22:99-104.
16. Kongmuang, U., J. M. Luk, and A. A. Lindberg. 1994. Comparison of three stool-processing methods for detection of Salmonella serogroups B, C2, and D by PCR. J. Clin. Microbiol. 32:3072-3074.
17. Lem, P., J. Spiegelman, B. Toye, and K. Ramotar. 2001. Direct detection of mecA, nuc and 16S rRNA genes in Bact/Alert blood culture bottles. Diagn. Microbiol. Infect. Dis. 41:165-168.
18. Luong, T. T., S. Ouyang, K. Bush, and C. Y. Lee. 2002. Type 1 capsule genes of Staphylococcus aureus are carried in a staphylococcal cassette chromosome genetic element. J. Bacteriol. 184:3623-3629.
19. Mongkolrattanothai, K., S. Boyle, T. Murphy, and R. S. Daum. 2004. Novel non-mecA-containing staphylococcal chromosomal cassette composite island containing pbp4 and tagF genes in a commensal staphylococcal species: a possible reservoir for antibiotic resistance islands in Staphylococcus aureus. Antimicrob. Agents Chemother. 48:1823-1836.
20. National Committee for Clinical Laboratory Standards. 2004. Performance standards for antimicrobial susceptibility testing; fourteenth informational supplement. M100-S14. National Committee for Clinical Laboratory Standards, Wayne, Pa.
21. National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, 6th ed. M7-A6. National Committee for Clinical Laboratory Standards, Wayne, Pa.
22. National Committee for Clinical Laboratory Standards. 2003. Performance standards for antimicrobial disk susceptibility tests; approved standards, 8th ed. M2-A8. National Committee for Clinical Laboratory Standards, Wayne, Pa.
23. National Nosocomial Infections Surveillance System. 2003. National Nosocomial Infections surveillance (NNIS) system report, data summary from January 1992 through June 2003, issued August 2003. Am. J. Infect. Control 31:481-498.
24. Nilsson, P., H. Alexandersson, and T. Ripa. 2005. Use of broth enrichment and real-time PCR to exclude the presence of methicillin-resistant Staphylococcus aureus in clinical samples: a sensitive screening approach. Clin. Microbiol. Infect. 11:1027-1034.
25. Reischl, V., H. J. Linde, M. Metz, B. Leppmeier, and N. Lehn. 2000. Rapid identification of methicillin-resistant Staphylococcus aureus and simultaneous species confirmation using real-time fluorescence PCR. J. Clin. Microbiol. 38:2429-2433.
26. Safdar, N., L. Narans, B. Gordon, and D. G. Maki. 2003. Comparison of culture screening methods for detection of nasal carriage of methicillin-resistant Staphylococcus aureus: a prospective study comparing 32 methods. J. Clin. Microbiol. 41:3163-3166.
27. Sewell, D., S. A. Potter, C. M. Jacobson, L. J. Strausbaugh, and T. T. Ward. 1993. Sensitivity of surveillance cultures for the detection of methicillin-resistant Staphylococcus aureus in a nursing-home care unit. Diagn. Microbiol. Infect. Dis. 17:53-56.
28. Simor, A. E., M. Ofner-Agostini, E. Bryce, A. McGeer, S. Paton, M. R. Mulvey, and the Canadian Hospital Epidemiology Committee and Canadian Nosocomial Infection Surveillance Program, Health Canada. 2002. Laboratory characterization of Methicillin-resistant Staphylococcus aureus in Canadian hospitals: results of 5 years of national surveillance, 1995-1999. J. Infect. Dis. 186:652-660.
29. Suh, K., B. Toye, P. Jessamine, F. Chan, and K. Ramotar. 1998. Epidemiology of methicillin-resistant Staphylococcus aureus in three Canadian tertiary-care centers. Infect. Control Hosp. Epidemiol. 19:395-400.
30. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Michelson, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33:2233-2239.
31. Warren, D. K., R. S. Liao, L. R. Merz, M. Eveland, and W. M. Dunne, Jr. 2004. Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J. Clin. Microbiol. 42:5578-5581.