Evaluation of the Cepheid GeneXpert® system for detecting Bacillus anthracis

Journal of Applied Microbiology

Volumn 100, Issue 5, 2006, Pages 1011-1016

  Ulrich, M P ^a   Christensen, D R ^a   Coyne, S R ^a   Craw, P D ^a   Henchal, E A ^a   Sakai, S H ^b   Swenson, D ^b   Tholath, J ^b   Tsai, J ^b   Weir, A F ^b   Norwood, D A ^a  

^a UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

^b CEPHEID   (United States)

Author keywords

Anthrax; Automated system; Bacillus anthracis; Genexpert ; Nucleic acid; Real time PCR; Sample processing

Indexed keywords

AUTOMATION; BACTERIA; BACTERIOLOGY; POLYMERASE CHAIN REACTION;

ANTHRAX; AUTOMATED SYSTEMS; B.ANTHRACIS; BACILLUS ANTHRACIS; CEPHEIDS; CROSS-REACTIVITY; GENEXPERT®; PCR DETECTION; REAL-TIME PCR; SAMPLE PROCESSING;

BIOMOLECULES;

NUCLEIC ACID;

ASSAY; BACTERIUM; INHIBITION; NUCLEIC ACID; POLYMERASE CHAIN REACTION; SPORE; VIRULENCE;

ARTICLE; AUTOMATION; BACILLUS ANTHRACIS; BACTERIAL SPORE; BACTERIUM DETECTION; COLONY FORMING UNIT; CONTROLLED STUDY; CROSS REACTION; EXTRACTION; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; RELIABILITY;

BACILLUS (BACTERIUM); BACILLUS ANTHRACIS; BACILLUS ANTHRACIS STR. AMES; BACTERIA (MICROORGANISMS);

EID: 33645841639     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2006.02810.x     Document Type: Article

References (19)

1. Bailey A.M. Pajak L. Fruchey I.S. Cowan C.A. Emanuel P.A. 2003 Robotic nucleic acid isolation using a magnetic bead resin and an automated liquid handler for biological agent simulants. J Assoc Lab Automation 8 113 120.
2. Belgrader P. Benett W. Hadley D. Richards J. Stratton P. Mariella R. Milanovich F. 1999 PCR detection of bacteria in seven minutes. Science 284 449 450.
3. Belgrader P. Okuzumi M. Pourahmadi F. Borkholder D.A. Northrup M.A. 2000 A microfluidic cartridge to prepare spores for PCR analysis. Biosens Bioelectron 14 849 852.
4. Christensen D.R. Hartman L.J. Loveless B.M. Frye M.S. Shipley M.A. Bridge D.L. House M.J. Richards M.J. Kaplan R.S. Garrison J. Baldwin C.D. Kulesh D.A. Norwood D.A. 2006 Detection of biological threat agents by real-time PCR: Comparison of assay performance on the R.A.P.I.D., the LightCycler, and the SmartCycler platforms. Clin Chem 52 141 145.
5. Coyne S.R. Craw P.D. Norwood D.A. Ulrich M.P. 2004 Comparative analysis of Schleicher and Schuell IsoCode Stix DNA isolation device and the Qiagen QIAamp DNA mini kit. J Clin Microbiol 42 4859 4862.
6. Exner M.M. Lewinski M.A. 2003 Isolation and detection of Borrelia burgdorferi DNA from cerebral spinal fluid, synovial fluid, blood, urine, and ticks using the Roche MagNA Pure system and real-time PCR. Diagn Microbiol Infect Dis 46 235 240.
7. Fiebelkorn K.R. Lee B.G. Hill C.E. Caliendo A.M. Nolte F.S. 2002 Clinical evaluation of an automated nucleic acid isolation system. Clin Chem 48 1613 1615.
8. Germer J.J. Lins M.M. Jensen M.E. Harmsen W.S. Ilstrup D.M. Mitchell P.S. Cockerill F.R. Patel R. 2003 Evaluation of the MagNA Pure LC instrument for extraction of hepatitis C virus RNA for the COBAS AMPLICOR hepatitis C virus test, version 2.0. J Clin Microbiol 41 3503 3508.
9. Hartman L.J. Coyne S.R. Norwood D.A. 2004 Development of a novel internal positive control for Taqman based assays. Mol Cell Probes 19 51 59.
10. Kessler H.H. Mühlbauer G. Stelzl E. Daghofer E. Santner B.I. Marth E. 2001 Fully automated nucleic acid extraction: MagNA Pure LC. Clin Chem 47 1124 1126.
11. Knepp J.H. Geahr M.A. Forman M.S. Valsamakis A. 2003 Comparison of automated and manual nucleic acid extraction methods for detection of enterovirus RNA. J Clin Microbiol 41 3532 3536.
12. de Kok J.B. Hendriks J.C. van Solinge W.W. Willems H.L. Mensink E.J. Swinkels D.W. 1998 Use of real-time quantitative PCR to compare DNA isolation methods. Clin Chem 44 2201 2204.
13. Kuske C.R. Banton K.L. Adorada D.L. Stark P.C. Hill K.K. Jackson P.J. 1998 Small-scale DNA sample preparation method for field PCR detection of microbial cells and spores in soil. Appl Environ Microbiol 64 2463 2472.
14. Loeffler J. Henke N. Hebart H. Schmidt D. Hagmeyer L. Schumacher U. Einsele H. 2000 Quantification of fungal DNA by using fluorescence resonance energy transfer and the Light Cycler system. J Clin Microbiol 38 586 590.
15. Petersen K. McMillan W. 2002 IVD systems in bioterrorism response. IVD Technology May 35
16. Raja S. Ching J. Xi L. Hughes S.J. Chang R. Wong W. McMillan W. Gooding W.E. et al. 2005 Technology for automated, rapid, and quantitative PCR or reverse transcription-PCR clinical testing. Clin Chem 51 882 890.
17. Smit M.L. Giesendorf B.A. Heil S.G. Ver J.A. Trijbels F.J. Blom H.J. 2000 Automated extraction and amplification of DNA from whole blood using a robotic workstation and an integrated thermocycler. Biotechnol Appl Biochem 32 121 125.
18. Smith K. Diggle M.A. Clarke S.C. 2003 Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples. J Clin Microbiol 41 2440 2443.
19. Wilson I.G. 1997 Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63 3741 3751.