Lack of direct DNA damage in human blood leukocytes and lymphocytes after in vitro exposure to high power microwave pulses

Bioelectromagnetics

Volumn 27, Issue 3, 2006, Pages 197-203

  Chemeris, N K ^a   Gapeyev, A B ^a   Sirota, N P ^a   Gudkova, O Yu ^a   Tankanag, A V ^a   Konovalov, I V ^b   Buzoverya, M E ^b   Suvorov, V G ^b   Logunov, V A ^b  

^a INSTITUTE OF CELL BIOPHYSICS   (Russian Federation)

^b RUSSIAN FEDERAL NUCLEAR CENTER VNIIEF   (Russian Federation)

Author keywords

Alkaline comet assay; Genotoxicity; High power RF pulses; Leukocytes; Lymphocytes

Indexed keywords

BLOOD; CELL CULTURE; DNA; ELECTROPHORESIS;

ALKALINE COMET ASSAY; DNA DAMAGES; GENOTOXICITIES; HIGH POWER; HIGH POWER RF PULSE; HUMAN BLOODS; LEUCOCYTES; MICROWAVE PULSE; POWER MICROWAVE; RF PULSE;

LYMPHOCYTES;

ADULT; ARTICLE; CELL ISOLATION; CELL SUSPENSION; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; EXCISION REPAIR; GENOTOXICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; LEUKOCYTE; LYMPHOCYTE; MALE; MICROWAVE RADIATION; NORMAL HUMAN; PRIORITY JOURNAL; RADIATION EXPOSURE; STEADY STATE; TEMPERATURE DEPENDENCE; WAVEFORM;

EID: 33645730500     PISSN: 01978462     EISSN: 1521186X     Source Type: Journal    
DOI: 10.1002/bem.20196     Document Type: Article

References (26)

1. Agee FJ, Baum CE, Prather WD, Lehr JM, O'Loughlin JP, Burger JW, Schoenberg JSH, Scholfield DW, Torres RJ, Hull JP, Gaudet JA. 1998. Ultra-wideband transmitter research. IEEE Trans Plasma Sci 26:860-873.
2. Albanese R, Blaschak J, Medina R, Penn J. 1994. Ultrashort electromagnetic signals: Biophysical questions, safety issues, and medical opportunities. Aviat Space Environ Med 65(Suppl 5):A116-A120.
3. Ames BN, Gold LS. 1991. Endogenous mutagens and the causes of aging and cancer. Mutat Res 250:3-16.
4. Bolshakov MA, Bugaev SP, Goncharic AO, Gunin AV, Evdokimov EV, Klimov AI, Korovin SD, Pegel IV, Rostov VV. 2000. Effect of high energy microwaves of nanosecond duration on several biological objects. Dokl Akad Nauk 371:691-695.
5. Bolshakov MA, Eltchaninov AA, Klimov AI, Knyazeva IR, Korovin SD, Pegel IV, Rostov VV, Voskresensky VV. 2001a. Effect of nanosecond HPM pulses on individual development of Drosophilas: Estimation of comparative contribution by microwave and X-ray components. In: Proc. of 5-th Korea-Russia International Symposium on Science and Technology. Vol. 2. Tomsk: Ivan Fedorov. pp 74-76.
6. Bolshakov MA, Klimov AI, Litvyakov NV. Kushakova YuA, Korovin SD, Rostov VV, Cherdyntseva NV. 2001b. Study of the effects of short exposure of high-power SHF pulses on tumor cells of mastocytoma P-815. In: Physiology of organisms in normal and extreme states. Tomsk: Ivan Fedorov. pp 130-133.
7. Bruskov VI, Malakhova LV, Masalimov ZK, Chernikov AV. 2002. Heat-induced formation of reactive oxygen species and 8-xoguanine, a biomarker of damage to DNA. Nucleic Acids Res 30:1354-1363.
8. Buschini A, Carboni P, Martino A, Poli P, Rossi C. 2003. Effects of temperature on baseline and genotoxicant-induced DNA damage in haemocytes of Dreissena polymorpha. Mutat Res 537:81-92.
9. Chemeris NK, Gapeyev AB, Sirota NP, Gudkova OYu, Kornienko NV, Tankanag AV, Konovalov IV, Buzoverya ME, Suvorov VG, Logunov VA. 2004. DNA damage in frog erythrocytes after in vitro exposure to a high peak-power pulsed electromagnetic Held. Mutat Res 558:27-34.
10. Creighton MO, Larsen LE, Stewart-DeHaan PJ, Jacobi JH, Sanwal M, Baskerville JC, Bassen HE, Brown DO, Trevithick JR. 1987. In vitro studies of microwave-induced cataract. II. Comparison of damage observed for continuous wave and pulsed microwaves. Exp Eye Res 45:357-373.
11. Devyatkov ND, Pletnev SD, Chernov ZS, Faikin VV, Bernashevsky GA, Shchitkov KG. 1994. Effect of low-energy pulses of EHF- and SHF-radiation of nanosecond duration with a high peak intensity on biological structures (malignant neoplasm). Dokl Akad Nauk 336:826-828.
12. Devyatkov ND, Pletnev SD, Betskii OV, Faikin VV 2000. The influence of low-energy high peak-power pulsed SHF-radiation of nanosecond duration on development of malignant neoplasm. Biomed Radioelectron 10:29-36.
13. Gapeyev AB, Lushnikov KV, Shumilina Ju V, Sirota NP, Sadovnikov VB, Chemeris NK. 2003. Effects of low-intensity extremely high frequency electromagnetic radiation on chromatin structure of lymphoid cells in vivo and in vitro. Radiats Biol Radioecol 43:87-92.
14. Gudkova OYu, Gudkov SV, Gapeyev AB, Bruskov VI, Rubanik AV, Chemeris NK. 2005. The study of the mechanisms of formation of reactive oxygen species in aqueous solutions on exposure to high peak-power pulsed electromagnetic radiation of extremely high frequencies. Biofizica 50:773-779.
15. Guetens G, De Boeck G, Highley M, van Oosterom AT, de Bruijn EA. 2002. Oxidative DNA damage: Biological significance and methods of analysis. Crit Rev Clin Lab Sci 39(4-5):331-457.
16. Guy AW, Chou CK, Lin JC. 1975. Microwave-induced acoustic effects in mammalian auditory systems and physical materials. Ann NY Acad Sci 247:194-218.
17. Hartmann A, Agurell E, Beevers C, Brendler-Schwaab S, Burlinson B, Clay P, Collins A, Smith A, Speit G, Thybaud V, Tice RR. 2003. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis 18:45-51.
18. Lebedev IV. 1961. Techniques and devices for super high frequencies, Vol. 1 Moscow-Leningrad: Gosenergoizdat.
19. Natarajan M, Vijayalaxmi, Szilagyi M, Roldan FN, Meltz ML. 2002. NF-κB DNA-binding activity after high peak power pulsed microwave (8.2 GHz) exposure of normal human monocytes. Bioelectromagnetics 23(4):271-277.
20. Olive PL, Banath JP, Durand RE. 1990. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay. Radiat Res 122:86-94.
21. Olsen RG, Lin JC. 1983. Microwave-induced pressure waves in mammalian brains. IEEE Trans Biomed Eng 30:289-294.
22. Pakhomov AG, Mathur SP, Doyle J, Stuck BE, Kiel JL, Murphy MR. 2000. Comparative effects of extremely high power microwave pulses and a brief CW irradiation on pacemaker function in isolated frog heart slices. Bioelectromagnetics 21:245-254.
23. Raslear TG, Akyel Y, Bates F, Belt M, Lu ST. 1993. Temporal bisection in rats: The effects of high-peak power pulsed microwave irradiation. Bioelectromagnetics 14:459-478.
24. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu J-C, Sasaki YF. 2000. Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206-221.
25. Tice RR, Hook GG, Donner M, McRee DI, Guy AW. 2002. Genotoxicity of radiofrequency signals. I. Investigation of DNA damage and micronuclei induction in cultured human blood cells. Bioelectromagnetics 23:113-126.
26. Vaks VL, Domrachev GA, Rodigin YuL, Selivanovsky DA, Spivak EI. 1994. Dissociation of water under an influence of the microwave electromagnetic irradiation. Izvestiya VUZ Radiophysics 37(1):149-154.