Response to mechanical strain in an immortalized pre-osteoblast cell is dependent on ERK1/2

Journal of Cellular Physiology

Volumn 207, Issue 2, 2006, Pages 454-460

  Fan, Xian ^a   Rahnert, Jill A ^a   Murphy, Tamara C ^a   Nanes, Mark S ^a   Greenfield, Edward M ^b   Rubin, Janet ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; CELL MARKER; ENDOTHELIAL NITRIC OXIDE SYNTHASE; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; NITRIC OXIDE; OSTEOCLAST DIFFERENTIATION FACTOR; TRANSCRIPTION FACTOR OSTERIX; TRANSCRIPTION FACTOR RUNX2; UNCLASSIFIED DRUG; VIRUS LARGE T ANTIGEN;

ANIMAL CELL; ARTICLE; CALVARIA; CELL CULTURE; CELL IMMORTALIZATION; CELL LINE; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; GENE EXPRESSION; MECHANICAL STRESS; MOUSE; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SIMIAN VIRUS 40; STRESS STRAIN RELATIONSHIP; STROMA CELL; TEMPERATURE SENSITIVITY;

ALKALINE PHOSPHATASE; ANIMALS; CALCITRIOL; CARRIER PROTEINS; CELL LINE, TRANSFORMED; CORE BINDING FACTOR ALPHA 1 SUBUNIT; GENE EXPRESSION; GLYCOPROTEINS; MEMBRANE GLYCOPROTEINS; MICE; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; NITRIC OXIDE SYNTHASE TYPE I; NITRIC OXIDE SYNTHASE TYPE II; NITRIC OXIDE SYNTHASE TYPE III; OSTEOBLASTS; OSTEOPROTEGERIN; PROTEIN KINASE INHIBITORS; PYRIDINES; RANK LIGAND; RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECEPTORS, TUMOR NECROSIS FACTOR; STRESS, MECHANICAL; STROMAL CELLS; TRANSCRIPTION FACTORS;

ANIMALIA; MURINAE; SIMIAN VIRUS 40;

EID: 33645670096     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/jcp.20581     Document Type: Article

References (42)

1. Bancroft GN, Sikavitsas VI, van den Dolder J, Sheffield TL, Ambrose CG, Jansen JA, Mikos AG. 2002. Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner. Proc Natl Acad Sci USA 99(20):12600-12605.
2. Bassey EJ, Rothwell MC, Littlewood JJ, Pye DW. 1998. Pre- and postmenopausal women have different bone mineral density responses to the same high-impact exercise. J Bone Miner Res 13(12):1805-1813.
3. Boppart MD, Kimmel DB, Yee JA, Cullen DM. 1998. Time course of osteoblast appearance after in vivo mechanical loading. Bone 23(5):409-415.
4. Boutahar N, Guignandon A, Vico L, Lafage-Proust MH. 2004. Mechanical strain on osteoblasts activates autophosphorylation of focal adhesion kinase and proline-rich tyrosine kinase 2 tyrosine sites involved in ERK activation. J Biol Chem 279(29):30588-30599.
5. Darimont C, Avanti O, Tromvoukis Y, Vautravers-Leone P, Kurihara N, Roodman GD, Colgin LM, Tullberg-Reinert H, Pfeifer AM, Offord EA, Mace K. 2002. SV40 T antigen and telomerase are required to obtain immortalized human adult bone cells without loss of the differentiated phenotype. Cell Growth Differ 13(2):59-67.
6. Davis ME, Cai H, Drummond GR, Harrison DG. 2001. Shear stress regulates endothelial nitric oxide synthase expression through c-Src by divergent signaling pathways. Circ Res 89(11):1073-1080.
7. Davis ME, Grumbach IM, Fukai T, Cutchins A, Harrison DG. 2004. Shear stress regulates endothelial nitric-oxide synthase promoter activity through nuclear factor {kappa}B binding. J Biol Chem 279(1):163-168.
8. Divieti P, Lanske B, Kronenberg HM, Bringhurst FR. 1998. Conditionally immortalized murine osteoblasts lacking the type 1 PTH/PTHrP receptor. J Bone Miner Res 13(12):1835-1845.
9. Dormady SP, Bashayan O, Dougherty R, Zhang XM, Basch RS. 2001. Immortalized multipotential mesenchymal cells and the hematopoietic microenvironment. J Hematother Stem Cell Res 10(1):125-140.
10. Fan X, Roy E, Zhu L, Murphy TC, Ackert-Bicknell C, Hart CM, Rosen C, Nanes MS, Rubin J. 2004. Nitric oxide regulates RANKL and OPG expression in bone stromal cells. Endocrinology 145(2):1-9.
11. Feuerbach D, Loetscher E, Buerki K, Sampath TK, Feyen JH. 1997. Establishment and characterization of conditionally immortalized stromal cell lines from a temperature-sensitive T-Ag transgenic mouse. J Bone Miner Res 12(2):179-190.
12. Harris SA, Enger RJ, Riggs BL, Spelsberg TC. 1995. Development and characterization of a conditionally immortalized human fetal osteoblastic cell line. J Bone Miner Res 10(2):178-186.
13. Heath JK, Rodan SB, Yoon K, Rodan GA. 1989a. Rat calvarial cell lines immortalized with SV-40 large T antigen: Constitutive and retinoic acid-inducible expression of osteoblastic features. Endocrinology 124(6):3060-3068.
14. Heath JK, Rodan SB, Yoon K, Rodan GA. 1989b. SV-40 large-T immortalization of embryonic bone cells: Establishment of osteoblastic clonal cell lines. Connect Tissue Res 20(1-4):15-21.
15. Hicok KC, Thomas T, Gori F, Rickard DJ, Spelsberg TC, Riggs BL. 1998. Development and characterization of conditionally immortalized osteoblast precursor cell lines from human bone marrow stroma. J Bone Miner Res 13(2):205-217.
16. Hukkanen MV, Platts LA, Fernandez De Marticorena I, O'Shaughnessy M, MacIntyre I, Polak JM. 1999. Developmental regulation of nitric oxide synthase expression in rat skeletal bone. J Bone Miner Res 14(6):868-877.
17. Ishijima M, Rittling SR, Yamashita T, Tsuji K, Kurosawa H, Nifuji A, Denhardt DT, Noda M. 2001. Enhancement of osteoclastic bone resorption and suppression of osteoblastic bone formation in response to reduced mechanical stress do not occur in the absence of osteopontin. J Exp Med 193(3):399-404.
18. Jat PS, Noble MD, Ataliotis P, Tanaka Y, Yannoutsos N, Larsen L, Kioussis D. 1991. Direct derivation of conditionally immortal cell lines from an H-2Kb-tsA58 transgenic mouse. Proc Natl Acad Sci USA 88(12):5096-5100.
19. Judex S, Zernicke RF. 2000. High-impact exercise and growing bone: Relation between high strain rates and enhanced bone formation. J Appl Physiol 88(6):2183-2191.
20. Judex S, Gross TS, Zernicke RF. 1997. Strain gradients correlate with sites of exercise-induced bone-forming surfaces in the adult skeleton. J Bone Miner Res 12(10):1737-1745.
21. Lengner CJ, Lepper C, van Wijnen AJ, Stein JL, Stein GS, Lian JB. 2004. Primary mouse embryonic fibroblasts: A model of mesenchymal cartilage formation. J Cell Physiol 200(3):327-333.
22. Li YJ, Batra NN, You L, Meier SC, Coe IA, Yellowley CE, Jacobs CR. 2004. Oscillatory fluid flow affects human marrow stromal cell proliferation and differentiation. J Orthop Res 22(6):1283-1289.
23. Machwate M, Zerath E, Holy X, Pastoureau P, Marie PJ. 1994. Insulin-like growth factor-I increases trabecular bone formation and osteoblastic cell proliferation in unloaded rats. Endocrinology 134(3):1031-1038.
24. MacKelvie KJ, Khan KM, Petit MA, Janssen PA, McKay HA. 2003. A school-based exercise intervention elicits substantial bone health benefits: A 2-year randomized controlled trial in girls. Pediatrics 112(6 Pt 1):e447.
25. Nanes MS, Rubin J, Titus L, Hendy GN, Catherwood BD. 1990. Interferon-gamma inhibits 1,25-dihydroxyvitamin D3-stimulated synthesis of bone GLA protein in rat osteosarcoma cells by a pretranslational mechanism. Endocrinology 127(2):588-594.
26. Plotkin LI, Mathov I, Aguirre JI, Parfitt AM, Manolagas SC, Bellido T. 2005. Mechanical Stimulation Prevents Osteocyte Apoptosis: Requirement Of Integrins, Src Kinases And Erks. Am J Physiol Cell Physiol.
27. Ragab AA, Nalepka JL, Bi Y, Greenfield EM. 2002. Cytokines synergistically induce osteoclast differentiation: Support by immortalized or normal calvarial cells. Am J Physiol Cell Physiol 283(3):C679-C687.
28. Rubin J, Fan X, Biskobing D, Taylor W, Rubin C. 1999. Osteoclastogenesis is repressed by mechanical strain in an in vitro model. J Orthop Res 17:639-645.
29. Rubin J, Murphy T, Nanes MS, Fan X. 2000. Mechanical strain inhibits expression of osteoclast differentiation factor by murine stromal cells. Am J Physiol Cell Physiol 278(6):C1126-C1132.
30. Rubin C, Xu G, Judex S. 2001. The anabolic activity of bone tissue, suppressed by disuse, is normalized by brief exposure to extremely low-magnitude mechanical stimuli. FASEB J 15(12):2225-2229.
31. Rubin J, Ackert-Bicknell C, Zhu L, Fan X, Murphy T, Nanes M, Marcus R, Holloway L, Beamer W, Rosen C. 2002a. Insulin like growth factor-I (IGF-I) regulates osteoprotegerin (OPG) and RANK ligand (RANKL) in vitro and osteoprotogerin in vivo. J Clin Endocrinol Metab 87(9):4273-4279.
32. Rubin J, Murphy T, Fan X, Goldschmidt M, Taylor W. 2002b. Mechanical strain inhibits RANKL expression through activation of ERK1/2 in bone marrow stromal cells. J Bone Miner Res 17:1452-1460.
33. Rubin J, Murphy TC, Zhu L, Roy E, Nanes MS, Fan X. 2003. Mechanical strain differentially regulates endothelial nitric-oxide synthase and receptor activator of nuclear kappa B ligand expression via ERK1/2 MAPK. J Biol Chem 278(36):34018-34025.
34. Smith SM, Wastney ME, Morukov BV, Larina IM, Nyquist LE, Abrams SA, Taran EN, Shih CY, Nillen JL, Davis-Street JE, Rice BL, Lane HW. 1999. Calcium metabolism before, during, and after a 3-mo spaceflight: Kinetic and biochemical changes. Am J Physiol 277(1 Pt 2):R1-R10.
35. Smith SM, Wastney ME, O'Brien KO, Morukov BV, Larina IM, Abrams SA, Davis-Street JE, Oganov V, Shackelford LC. 2005. Bone markers, calcium metabolism, and calcium kinetics during extended-duration space flight on the mir space station. J Bone Miner Res 20(2):208-218.
36. Srinivasan S, Weimer DA, Agans SC, Bain SD, Gross TS. 2002. Low-magnitude mechanical loading becomes osteogenic when rest is inserted between each load cycle. J Bone Miner Res 17(9):1613-1620.
37. Udagawa N, Takahashi N, Akatsu T, Sasaki T, Yamaguchi A, Kodama H, Martin TJ, Suda T. 1989. The bone marrow-derived stromal cell lines MC3T3-G2/PA6 and ST2 support osteoclast-like cell differentiation in cocultures with mouse spleen cells. Endocrinology 125(4):1805-1813.
38. Van't Hof RJ, Ralston SH. 2001. Nitric oxide and bone. Immunology 103(3):255-261.
39. You J, Reilly GC, Zhen X, Yellowley CE, Chen Q, Donahue HJ, Jacobs CR. 2001. Osteopontin gene regulation by oscillatory fluid flow via intracellular calcium mobilization and activation of mitogen-activated protein kinase in MC3T3-E1 osteoblasts. J Biol Chem 276(16):13365-13371.
40. Zaman G, Pitsillides AA, Rawlinson SC, Suswillo RF, Mosley JR, Cheng MZ, Platts LA, Hukkanen M, Polak JM, Lanyon LE. 1999. Mechanical strain stimulates nitric oxide production by rapid activation of endothelial nitric oxide synthase in osteocytes. J Bone Miner Res 14(7):1123-1131.
41. Zhu W, Diwan AD, Lin JH, Murrell GA. 2001. Nitric oxide synthase isoforms during fracture healing. J Bone Miner Res 16(3):535-540.
42. Ziros PG, Gil AP, Georgakopoulos T, Habeos I, Kletsas D, Basdra EK, Papavassiliou AG. 2002. The bone-specific transcriptional regulator Cbfa1 is a target of mechanical signals in osteoblastic cells. J Biol Chem 277(26):23934-23941.