Development and characterization of conditionally immortalized osteoblast precursor cell lines from human bone marrow stroma

Journal of Bone and Mineral Research

Volumn 13, Issue 2, 1998, Pages 205-217

  Hicok, Kevin C ^a   Thomas, Thierry ^a   Gori, Francesca ^a   Rickard, David J ^a   Spelsberg, Thomas C ^b   Riggs, B Lawrence ^a  

^a MAYO CLINIC   (United States)

^b MAYO GRADUATE SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; ASCORBIC ACID; CALCITRIOL; COLLAGEN TYPE 1; DEXAMETHASONE; LIPOPROTEIN LIPASE; MESSENGER RNA; OSTEOCALCIN; PARATHYROID HORMONE RECEPTOR;

ADIPOCYTE; ANIMAL CELL; ARTICLE; BONE MARROW CELL; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL STRAIN 3T3; CONTROLLED STUDY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; OSTEOBLAST; RETROVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STEM CELL;

ADIPOCYTES; ANTIGENS, VIRAL, TUMOR; ASCORBIC ACID; BONE MARROW CELLS; CALCITRIOL; CELL DIFFERENTIATION; CELL DIVISION; CELL LINE; DEXAMETHASONE; GENETIC VECTORS; GLYCEROPHOSPHATES; HUMANS; IMMUNOHISTOCHEMISTRY; MUTATION; OSTEOBLASTS; OSTEOGENESIS; RETROVIRUSES, SIMIAN; RNA, MESSENGER; STROMAL CELLS; TRANSFECTION;

EID: 0031893494     PISSN: 08840431     EISSN: None     Source Type: Journal    
DOI: 10.1359/jbmr.1998.13.2.205     Document Type: Article

References (78)

1. Friedenstein AJ 1976 Precursor cells of mechanocytes. Int Rev Cytol 47:327-355.
2. Owen M 1985 Lineage of osteogenic cells and their relationship to the stromal system. In: Peck WA (ed.) Bone and Mineral Research, Vol 3. Elsevier, Amsterdam, The Netherlands, pp. 1-23.
3. Owen ME, Cave J, Joyner CJ 1987 Clonal analysis in vitro of osteogenic differentiation of marrow CFU-F. J Cell Sci 87:731-738.
4. Ashton BA, Allen TD, Howlett CR, Eaglesom CC, Hattori A, Owen ME 1980 Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo. Clin Orthop 151:294-307.
5. Aubin JE, Turksen K, Heersche JNM 1993 Osteoblastic cell lineage. In: Noda M (ed.) Cellular and Molecular Biology of Bone. Academic Press, San Diego, CA, U.S.A. pp. 1-46.
6. Triffit JT 1996 The stem cell of the osteoblast. In: Bilezikian J, Raisz L, Rodan G (eds.) Principles of Bone Biology. Academic Press, San Diego, CA, U.S.A. pp. 39-50.
7. Owen ME and Friedenstein AJ 1988 Stromal stem cells: Marrow-derived osteogenic precursors. In: Evered D, Harnett S (eds.) Cell and Molecular Biology of Vertebrate Hard Tissues. Wiley, Chichester, U.K., pp. 42-60.
8. Grigoriadis AE, Heersche JNM, Aubin JE 1988 Differentiation of muscle, fat, cartilage and bone from progenitor cells present in a bone-derived clonal cell population: Effect of Dex. J Cell Biol 106:2139-2151.
9. Pereira RF, Halford KW, O-Hara MD, Leeper DB, Sokolov BP, Pollard MD, Bagasra O, Prockop DJ 1995 Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice. Proc Natl Acad Sci USA 92:4857-4861.
10. Wakitani S, Saito T, Caplan AI 1995 Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve 18:1417-1426.
11. Haynesworth SE, Goshima J, Goldberg VM, Caplan AI 1992 Characterization of cells with osteogenic potential from human marrow. Bone 13:81-88.
12. Rickard DJ, Kassem M, Hefferan TE, Sarkar G, Spelsberg TC, Riggs BL 1996 Isolation and characterization of osteoblast precursor cells from human bone marrow. J Bone Miner Res 11:312-324.
13. Cheng S, Yang JW, Rifas L, Zhang S, Avioli LV 1994 Differentiation of human bone marrow osteogenic stromal cells in vitro: Induction of the osteoblast phenotype by Dex. Endocrinology 134:277-286.
14. Spelsberg TC, Harris SA, Riggs BL 1995 Immortalized osteoblast cell systems (new human fetal osteoblast systems). Calcif Tissue Int 56 (Suppl 1):S18-S21.
15. Hassager C, Fitzpatrick LA, Spencer EM, Riggs BL, Conover CA 1992 Basal and regulated secretion of insulin-like growth factor binding proteins in osteoblast-like cells is cell line specific. J Clin Endocrinol Metab 75:228-233.
16. Okazaki R, Conover CA, Harris SA, Spelsberg TC, BL Riggs 1995 Normal human osteoblast-like cells consistently express genes for insulin-like growth factors I and II but transformed human osteoblast cell lines do not. J Bone Miner Res 10:788-795.
17. b-tsA58 transgenic mouse. J Cell Phys 167:523-538.
18. Benayahu D, Fried A, Zipori D, Wientroub S 1991 Subpopulations of marrow stromal cells share a variety of osteoblastic markers. Calcif Tissue Int 49:202-207.
19. Fried A, Benayahu D 1996 Dexamethasone regulation of marrow stromal-derived osteoblastic cells. J Cell Biochem 62:476-483.
20. Okuyama R, Yanai N, Obinata M 1995 Differentiation capacity toward mesenchymal cell lineages of bone marrow stromal cells established from temperature-sensitive SV40 T-antigen gene transgenic mouse. Exp Cell Res 218:424-429.
21. Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS 1990 Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143:420-430.
22. Liu, F, Malaval L, Gupta AK, Aubin JE 1994 Simultaneous detection of multiple bone-related mRNAs and protein expression during osteoblast differentiation: Polymerase chain reaction and immunocytochemical studies at the single cell level. Dev Biol 166:220-234.
23. Stein GS, Lian JB 1993 Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. Endocr Rev 14:424-442.
24. Aubin JE, Liu F 1996 The osteoblast lineage. In: Bilezikian J, Raisz L, Rodan G (eds.) Principles of Bone Biology. Academic Press, San Diego, CA, U.S.A., pp. 51-68.
25. 3 stimulates adipocyte differentiation in cultures of fetal rat calvaria cells: Comparison with the effects of Dex. Endocrinology 134:2221-2229.
26. Bellows CG, Aubin JE 1989 Determination of numbers of osteoprogenitors present in isolated fetal rat calvaria cells in vitro. Dev Biol 133:8-13.
27. Rickard DJ, Sullivan TA, Shenker BJ, Leboy PS, Kazhdan I 1994 Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by Dex and BMP-2. Dev Biol 161:218-228.
28. Bruder SP, Jaiswal N, Haynesworth SE 1997 Growth kinetics, self-renewal and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem 64:278-294.
29. Jaiswal N, Haynesworth SE, Caplan AI Bruder SP 1997 Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 64:295-312.
30. Haynesworth SE, Baber MA, Caplan AI 1996 Cytokine expression by human marrow derived mesenchymal progenitor cells in vitro: Effects of dexamethasone and IL-1α. J Cell Physiol 166:585-592.
31. Cheng S-L, Zhang S-F, Avioli LV 1996 Expression of bone matrix proteins during dexamethasone-induced mineralization of human bone marrow stromal cells. J Cell Biochem 61:182-193.
32. Kassem M, Risteli L, Mosekilde L, Melsen F, Eriksen EF 1991 Fonnation of osteoblast-like cells from human mononuclear bone marrow cultures. APMIS 99:269-274.
33. Leboy PS, Beresford JN, Devlin C, Owen ME 1991 Dcx induction of osteoblast mRNAs in rat marrow stromal cell cultures. J Cell Physiol 146:370-378.
34. Beresford JN, Bennett JH, Devlin C, Leboy PS, Owen ME 1992 Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J Cell Sci 102:341-351.
35. Maniatopoulos C, Sodek J, Melcher AH 1988 Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res 254:317-330.
36. Simmons PJ, Torok-Storb B 1991 Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 78:55-62.
37. Long MW, Robinson JA, Ashcraft EA, Mann KG 1995 Regulation of human bone marrow-derived osteoprogenitor cells by osteogenic growth factors. J Clin Invest 95:881-887.
38. Cepko CL, Roberts BE, Mulligan RC 1984 Construction and application of a highly transmissible murine retrovirus shuttle vector. Cell 37:1053-1062.
39. Jat PS, Sharp PA 1989 Cell lines established by a temperature-sensitive simian virus 40 large T-antigen gene are growth restricted at the nonpermissive temperature. Mol Cell Biol 9:1672-1681.
40. Miller AD, Buttimore C 1986 Redesign of retrovirus packing cell lines to avoid recombination leading to helper virus production. Mol Cell Biol 6:2895-2902.
41. Harris SA, Enger RJ, Riggs BL, Spelsberg TC 1995 Development and characterization of a conditionally immortalized human fetal osteoblastic cell line. J Bone Miner Res 10:178-186.
42. Bodine PVN, Trailsmith M, Komm BS 1996 Development and characterization of a conditionally transformed adult human osteoblastic cell line. J Bone Miner Res 11:806-819.
43. Howlett CR, Cave J, Williamson M, Farmer J, Ali SY, Bab I, Owen ME 1986 Mineralization in in vitro cultures of rabbit marrow stromal cells. Clin Orthop 213:251-263.
44. Benz EW Jr, Getz MJ, Wells DJ, Moses HL 1977 Nuclear RNA polymerase activities and poly(A)-containing mRNA accumulation in cultured AKR mouse embryo cells stimulated to proliferate. Exp Cell Res 108:157-165.
45. Chomczynski P, Sacchi N 1987 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
46. Puzas JE, Brand JS 1985 Bone cell phosphotyrosine phosphatase: Characterization and regulation by calcitropic hormones. Endocrinology 116:2463-2468.
47. Schenk RK, Olah AJ, Herrmann W 1984 Preparation of calcified tissues for light microscopy. In: Dickson GR (ed.) Methods of Calcified Tissue Preparation. Elsevier, Amsterdam, The Netherlands.
48. Bennett JH, Joyner CJ, Triffit JT, ME Owen 1991 Adipocyte cells cultured from marrow have osteogenic potential. J Cell Sci 99:131-139.
49. Houghton A, Oyajobi BO, Gravely RM, Russell RGG, Stringer BMJ 1995 The generation and characterization of novel conditionally immortalized osteoprogenitor cell lines derived from human hone marrow stromal cells by retroviral temperature sensitive oncogene transduction. J Bone Miner Res 10 (Suppl 1):M241 (abstract).
50. Schmidt A, Diascro DD, Vogel R, Witherup KM, Pitzenberger SM, Rutledge S-J, Rodan GA 1996 An unusually high fatty acid content in rabbit serum is responsible for the differentiation of osteoblasts into adipocyte-like cells. J Bone Miner Res 11 (Suppl 1):M553 (abstract).
51. Chou JY 1989 Differentiated mammalian cell lines immortalized by temperature-sensitive tumor viruses. Mol Endocrinol 3:1511-1514.
52. Mathieu E, Schoeters G, Vander Plaetse, Merregaert J 1992 Establishment of an osteogeneic cell line derived from adult mouse bone marrow stroma by use of a recombinant retrovirus. Calcif Tissue Int 50:362-371.
53. Moritz T, Patel VP, Williams DA 1994 Bone marrow extracellular matrix molecules improve gene transfer into human hematopoietic cells via retroviral vectors. J Clin Invest 93:1451-1457.
54. Keeting PE, Scott RE, Colvard DS, Anderson MA, Oursler MJ, Spelsberg TC, Riggs BL 1992 Development and characterization of a rapidly proliferating, well-differentiated cell line derived from normal adult human osteoblast-like cells transfected with SV40 large T antigen. J Bone Miner Res 7:127-136.
55. Chiba H, Sawada N, Ono T, Ishii S, Mori M 1993 Establishment and characterization of a simian virus 40-immortalized osteoblastic cell line from normal human bone. Jpn J Cancer Res 84:290-297.
56. Bruder SP, Caplan AI 1989 Cellular and molecular events during embryonic bone development. Connect Tissue Res 20:65-71.
57. Yamaguchi A, Kahn AJ 1991 Clonal osteogenic cell lines express myogenic and adipocytic developmental potential. Calcif Tissue Int 49:221-225.
58. Subramaniam M, Colvard D, Keeting PE, Rasmussen K, Riggs BL, Spelsberg TC 1992 Glucocorticoid regulation of alkaline phosphatase, osteocalcin, and proto-oncogenes in normal human osteoblast-like cells. J Cell Biochem 50:411-424.
59. 3, parathyroid hormone and glucocorticoid. Metab Bone Dis Rel Res 5:229-234.
60. Stromstedt P-E, Poellinger L, Gustafsson J-A, Carlstedt-Duke J 1991 The glucocorticoid receptor binds to a sequence overlapping the TATA box of the human osteocalcin promoter: A potential mechanism for negative regulation. Mol Cell Biol 11:3379-3383.
61. Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G 1997 Osf2/Cbfal: A transcriptional activator of osteoblast differentiation. Cell 89:747-754.
62. Cockayne D, Sterling KM, Shull S, Mintz KP, Illeyne S, Cutroneo KR 1986 Glucocorticoids decreases the synthesis of type I procollagen mRNAs. Biochemistry 25:3202-3209.
63. Weiner FR, Czaya MJ, Jefferson DM, Giambrone M-A, Tur-Kaspa R, Reid LM, Zern MA 1987 The effects of Dex on in vitro collagen gene expression. J Biol Chem 262:6955-6958.
64. Canalis E 1983 Effect of glucocorticoids on type I collagen synthesis, alkaline phosphatase activity and deoxyribo-nucleic acid content in cultured rat calvaria. Endocrinology 112:931-939.
65. Dietrich JW, Canalis EM, Maina DM, Raisz LG 1979 Effects of glucocorticoids on fetal rat bone collagen synthesis in vitro. Endocrinology 104:715-721.
66. Hahn Tj, Halstead LR, Teitelboum SL, Hahn BH 1979 Altered mineral metabolism in glucocorticoid-induced osteopenia, effect of 25-hydroxyvitamin D administration. J Clin Invest 64:655-665.
67. Dykman TR, Gluck OS, Murphy WA, Hahn TJ, Hahn BH 1985 Evaluation of factors associated with glucocorticoid-induced osteopenia in patients with rheumatic diseases. Arthritis Rheum 28:361-368.
68. 2 in the recruitment of bone marrow mesenchymal cells to the osteoblastic lineage: Positive and negative effects. Calcif Tissue Int 59:154-162.
69. Boden SD, Hair G, Titus L, Racine M, McCuaig K, Wozney JM, Nanes MS 1997 Glucocorticoid-induced differentiation of fetal rat calvarial osteoblasts in mediated by bone morphogenetic protein-6. Endocrinology 138:2820-2828.
70. Malaval L, Modrowski D, Gupta AK, Aubin JE 1994 Cellular expression of bone-related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures. J Cell Phys 15:555-572.
71. Pockwinse SM, Stein JL, Lian JB, Stein GS 1995 Developmental stage-specific cellular responses to vitamin D and glucocorticoids during differentiation of the osteoblast phenotype: Interrelationship of morphology and gene expression by in situ hybridization. Exp Cell Res 216:244-260.
72. Fried A, Benayahu D, Wientroub S 1993 Marrow stoma-derived osteogenic clonal cell lines: Putative stages in osteoblastic differentiation. J Cell Phys 155:472-482.
73. Vilamitjana-Amedee J, Bareille R, Rouais F, Caplan AI, Harmond M-F 1993 Human bone marrow stromal cells express an osteoblastic phenotype in culture. In Vitro Cell Dev Biol 29A:699-707.
74. Dorheim M-A, Sullivan M, Dandapani V, Wu X, Hudson J, Segarini PR, Rosen DM, Aulthouse AL, Gimble JM 1993 Osteoblastic gene expression during adipogenesis in hematopoietic supporting murine bone marrow stromal cells. J Cell Phys 154:317-328.
75. Gimble JM, Morgan C, Kelly K, Wu X, Dandapani V, Wang C-S, Rosen V 1995 Bone morphogenetic proteins inhibit adipocyte differentiation by bone marrow stromal cells. J Cell Biochem 58:393-402.
76. Gimble JM, Robinson CE, Wu X, Kelly KA 1996 The function of adipocytes in the bone marrow stroma: An update. Bone 19:421-428.
77. Vu D, Ong JM, Clemens TL, Kern PA 1996 1,25-Dihydroxyvitamin D induces lipoprotein lipase expression in 3T3-L1 cells in association with adipocyte differentiation. Endocrinology 137:1540-1544.
78. Sato M, Hiragun A 1988 Demonstration of 1α,25-dihydroxyvitamin D3 receptor-like molecule in ST 13 and 3T3-L1 preadipocytes and its inhibitory effects on preadipocyte differentiation. J Cell Physiol 135:545-550.