Analysis of the changes in the 1H NMR spectral pattern of perchloric acid extracts of C6 cells with growth

NMR in Biomedicine

Volumn 19, Issue 2, 2006, Pages 223-230

  Valverde, D ^a   Quintero, M R ^a   Candiota, A P ^a   Badiella, Ll ^a   Cabanas M E ^a   Arús, Carles ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Glutamine; Malignancy marker; Myo inositol; Proliferation rate; Tumour growth

Indexed keywords

AMINO ACIDS; CELL CULTURE; MAGNETIC RESONANCE SPECTROSCOPY; METABOLITES; NUCLEAR MAGNETIC RESONANCE; STATISTICAL METHODS; TUMORS;

CELL PROLIFERATION; GLUTAMINE; MALIGNANCY MARKER; MYO-INOSITOL; PERCHLORIC ACID;

CELLS;

CREATINE; GLUTAMINE; INOSITOL; PERCHLORIC ACID; TUMOR MARKER; PROTON; TISSUE EXTRACT;

ANIMAL CELL; ANIMAL MODEL; ARTICLE; ASTROCYTE; CELL PROLIFERATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; GLIOMA CELL; GROWTH CURVE; IN VITRO STUDY; IN VIVO STUDY; METABOLITE; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; RAT; RECORDING; STATISTICAL SIGNIFICANCE; TUMOR CELL CULTURE; TUMOR GROWTH; ANIMAL; GLIOMA; METABOLIC CLEARANCE RATE; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PATHOLOGY; PATHOPHYSIOLOGY; TUMOR CELL LINE;

ANIMALIA;

ANIMALS; CELL LINE, TUMOR; CELL PROLIFERATION; GLIOMA; MAGNETIC RESONANCE SPECTROSCOPY; METABOLIC CLEARANCE RATE; PERCHLORIC ACID; PROTONS; RATS; TISSUE EXTRACTS; TUMOR MARKERS, BIOLOGICAL;

EID: 33645511049     PISSN: 09523480     EISSN: 10991492     Source Type: Journal    
DOI: 10.1002/nbm.1024     Document Type: Article

References (32)

1. 1H MR spectroscopy of brain tumours and masses. NMR Biomed. 2003; 16: 123-131.
2. Herminghaus S, Pilatus U, Möller-Hartmann W, Raab P, Lanfermann H, Schlote W, Zanella FE. Increased choline levels coincide with enhanced proliferative activity of human neuroepithelial brain tumours. NMR Biomed. 2002; 15: 385-392.
3. Shimizu H, Kumabe T, Shirane R, Yoshimoto T. Correlation between choline level measured by proton MR spectroscopy and Ki-67 labeling index in gliomas. AJNR Am. J. Neuroradiol. 2000; 21: 659-665.
4. Castillo M, Smith JK, Kwock L. Correlation of myo-inositol levels and grading of cerebral astrocytomas. AJNR Am. J. Neuroradiol. 2000; 21: 1645-1649.
5. Raghavan R, Steart PV, Weller RO. Cell proliferation patterns in the diagnosis of astrocytomas, anaplasic astrocytomas and glioblastoma multiforme: a Ki-67 study. Neuropathol. Appl. Neurobiol. 1990; 16: 123-133.
6. Sallinen PK, Haapasalo HK, Visakorpi T, Helen PT, Rantala IS, Isola JJ, Helen HJ. Prognostication of astrocytoma patient survival by Ki-67 (MIB-1), PCNA and S-phase fraction using archival paraffin-embedded samples. J. Pathol. 1994; 174: 275-282.
7. Rémy C, Arús C, Ziegler A, Sam Lai E, Moreno A, Le Fur Y, Décorps M. In vivo, ex vivo and in vitro one- and two-dimensional nuclear magnetic resonance spectroscopy of an intracerebral glioma in rat brain: assignment of resonances. J. Neurochem. 1994; 62: 166-179.
8. Barba I, Cabañas ME, Arús C. The relationship between nuclear magnetic resonance-visible lipids, lipid droplets and cell proliferation in cultured C6 cells. Cancer Res. 1999; 59: 1861-1868.
9. 1H magnetic resonance spectroscopic imaging: comparison with maps of metabolites. Cancer Res. 2001; 61: 6524-6531.
10. Freshney RI. Culture of Animal Cells. A Manual of Basic Technique, 4th edn. Wiley-Liss: New York, 2000; 319.
11. 1H MRS of human brain tumour cystic fluid: contribution of macromolecules. MAGMA, Magn. Reson. Mater. Phy. 2004; 17: 36-46.
12. 1H MRS markers of tumour growth in intrasplenic tumours and liver metastasis induced by injection of HT-29 cells in nude mice spleen. NMR Biomed. 1998; 11: 93-106.
13. 1H NMR spectroscopy. NMR Biomed. 2002; 15: 37-44.
14. Flögel U, Leibfritz D. Alterations in glial cell metabolism during recovery from chronic osmotic stress. Neurochem. Res. 1998; 23: 1553-1561.
15. Lilienthal JL, Zierler KL, Folk BP, Buka R, Riley MJ. A reference base and system for analysis of muscle constituents. J. Biol. Chem. 1950; 182: 501-508.
16. Candiota A. Contribució a la millora del diagnòstic i de la valoració pronòstica de tumors cerebrals. PhD Thesis, Universitat Autònoma de Barcelona, 2004.
17. 1H NMR spectroscopy of colon tumors and normal mucosal biopsies; elevated taurine levels and reduced polyethyleneglycol absorption in tumors may have diagnostic significance. NMR Biomed. 1993; 6: 111-118.
18. Rubin A, Terasaki M, Sanui H. Major intracellular cations and growth control: correspondence among magnesium content, protein synthesis and the onset of DNA synthesis in BALB/c3T3 cells. Proc. Natl. Acad. Sci. USA 1979; 76: 3917-3921.
19. Silva F, Guimaraes E, Grivicich I, Trinidade V, Guaragna R, Borojevic R, Guma F. Hepatic stellate cell activation in vitro: cell cycle arrest at G2/M and modification of cell mobility. J. Cell. Biochem. 2003; 90: 387-396.
20. Majós C, Alonso J, Aguilera C, Serrallonga M, Pérez-Martín J, Acebes JJ, Arús C, Gili J. Proton magnetic resonance spectroscopy ((1)H MRS) of human brain tumours: assessment of differences between tumour types and its applicability in brain tumour categorization. Eur. Radiol. 2003; 13: 582-591.
21. Candiota A, Majós C, Julià-Sapé M, Cabañas M, Mercadal G, Acebes J, Moreno-Torres A, Griffiths J, Arús C. M-inositol and glycine content measuring for grading astrocytic tumours with in vivo MRS. MAGMA, Magn. Reson. Mater. Phy. 18(Suppl. 7): S193, 2005.
22. 31P-NMR spectroscopy. Am. J. Physiol. 1996; 270: 160-169.
23. Moreno A, López LA, Fabra A, Arús C. 1H MRS markers of tumour growth in intrasplenic tumours and liver metastasis induced by injection of HT-29 cells in nude mice spleen. NMR Biomed. 1998; 11: 93-106.
24. Odoom JE, Kemp GJ, Radda GK. The regulation of total creative content in a myoblast cell line. Mol. Cell. Biochem. 1996; 158: 179-188.
25. Erecinska M, Silver IA. The role of glial cells in regulation of neurotransmitter amino acids in the external environment. I. Transmembrane electrical and ion gradients and energy parameters in cultured glial-derived cell lines. Brain Res. 1986; 369: 193-202.
26. Urenjak J, Williams SR, Gadian DG, Noble M. Proton nuclear magnetic resonance spectroscopy unambiguously identifies different neural cell types. J. Neurosci. 1993; 13: 981-989.
27. Florian CL, Preece NE, Bhakoo KK, Williams SR. Characteristic metabolic profiles revealed by 1H NMR spectroscopy for three types of human brain and nervous system tumours. NMR Biomed. 1996; 8: 253-264.
28. Barba I, Moreno A, Martínez-Pérez I, Tate AR, Cabañas ME, Baquero M, Capdevila A, Arús C. Magnetic resonance spectroscopy of brain hemangiopericytomas: high myoinositol concentrations and discrimination from meningiomas. J. Neurosurg. 2001; 94: 55-60.
29. Chen JH, Enloe BM, Weybright P, Campbell N, Dorfman D, Fletcher D, Cory DG, Singer S. Biochemical correlates of thiazolidinedione-induced adipocyte differentiation by high-resolution magic angle spinning NMR spectroscopy. Magn. Reson. Med. 2002; 48: 602-610.
30. 1H NMR spectra from perchloric acid extracts of human brain tumour biopsies. Magn. Reson. Med. 1998; 39: 869-877.
31. 31P NMR analyses of phosphocholine metabolism in rat glioma cells. Magn. Reson. Med. 1994; 32: 310-318.
32. Rosi A, Grande S, Luciani AM, Barone P, Mlynarik V, Viti V, Guidoni L. (1H) MRS studies of signals from mobile lipids and from lipid metabolites: comparison of the behavior in cultured tumor cells and in spheroids. NMR Biomed. 2004; 17: 76-91.