Human TRPC6 expressed in HEK 293 cells forms non-selective cation channels with limited Ca2+ permeability

Journal of Physiology

Volumn 572, Issue 2, 2006, Pages 359-377

  Estacion, Mark ^a   Sinkins, William G ^b   Jones, Stephen W ^b   Applegate, Milana A B ^a   Schilling, William P ^a,b  

^a METROHEALTH MEDICAL CENTER   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1 [[6 (3 METHOXYESTRA 1,3,5(10) TRIEN 17BETA YL)AMINO]HEXYL] 1H PYRROLE 2,5 DIONE; 1 [[6 (3 METHOXYESTRA 1,3,5(10) TRIEN 17BETA YL)AMINO]HEXYL] 2,5 PYRROLIDINEDIONE; 1,6 HEXAMETHYLENEDICARBAMIC ACID BIS(CYCLOHEXANONE OXIME) ESTER; CALCIUM ACTIVATED POTASSIUM CHANNEL; CALCIUM ION; CATION CHANNEL; TRANSIENT RECEPTOR POTENTIAL CHANNEL; CALCIUM; G PROTEIN COUPLED RECEPTOR; GLYCEROL 2 ACETATE 1 OLEATE; INWARDLY RECTIFYING POTASSIUM CHANNEL; LIPOPROTEIN LIPASE; PHOSPHOLIPASE C; SODIUM; TRANSIENT RECEPTOR POTENTIAL CHANNEL 6; TRIACYLGLYCEROL LIPASE;

ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CELL MEMBRANE DEPOLARIZATION; CELL MEMBRANE PERMEABILITY; CELL MEMBRANE POTENTIAL; CELL STRAIN HEK293; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EMBRYO; HUMAN; HUMAN CELL; HYPERPOLARIZATION; PRIORITY JOURNAL; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; CYTOSOLIC FRACTION; DEPOLARIZATION; ENZYME ACTIVITY; HEK293 CELL LINE; IMAGE ANALYSIS; KINETICS; MEMBRANE DEPOLARIZATION; MEMBRANE PERMEABILITY; MEMBRANE POTENTIAL; MEMBRANE TRANSPORT; POTASSIUM CURRENT; SIGNAL TRANSDUCTION;

CALCIUM; CELL LINE; CELL MEMBRANE PERMEABILITY; CYCLOHEXANONES; DIGLYCERIDES; ELECTROPHYSIOLOGY; ESTRENES; HUMANS; KIDNEY; MEMBRANE POTENTIALS; MODELS, BIOLOGICAL; PATCH-CLAMP TECHNIQUES; PHOSPHODIESTERASE INHIBITORS; POTASSIUM; PROTEASE INHIBITORS; PYRROLIDINONES; SIGNAL TRANSDUCTION; SODIUM; TRPC CATION CHANNELS;

EID: 33645498890     PISSN: 00223751     EISSN: 14697793     Source Type: Journal    
DOI: 10.1113/jphysiol.2005.103143     Document Type: Article

References (44)

1. +. Am J Physiol Heart Circ Physiol 279, H679-H691.
2. Artigas P & Gadsby DC (2004). Large diameter of palytoxin-induced Na/ K pump channels and modulation of palytoxin interaction by Na/K pump ligands. J Gen Physiol 123, 357-376.
3. 2+ influx. J Biol Chem 280, 12908-12916.
4. Bleasdale JE, Thakur NR, Gremban RS, Bundy GL, Fitzpatrick FA, Smith RJ & Bunting S (1990). Selective inhibition of receptor-coupled phospholipase C-dependent processes in human platelets and polymorphonuclear neutorphils. J Pharmacol Exp Ther 255, 756-768.
5. q class of G protein. J Biol Chem 272, 29672-29680.
6. Chu X, Tong Q, Cheung JY, Wozney J, Conrad K, Mazack V, Zhang W, Stahl R, Barber DL & Miller BA (2004). Interaction of TRPC2 and TRPC6 in erythropoietin modulation of calcium influx. J Biol Chem 279, 10514-10522.
7. Clapham DE (2003). TRP channels as cellular sensors. Nature 426, 517-524.
8. Corteling RL, Li S, Giddings J, Westwick J, Poll C & Hall IP (2004). Expression of transient receptor potential C6 and related transient receptor potential family members in human airway smooth muscle and lung tissue. Am J Respir Cell Mol Biol 30, 145-154.
9. Dietrich A, Schnitzler MM, Emmel J, Kalwa H, Hofmann T & Gudermann T (2003). N-linked protein glycosylation is a major determinant for basal TRPC3 and TRPC6 channel activity. J Biol Chem 278, 47842-47852.
10. 2+ permeability. FASEB J 19, A1163.
11. Estacion M, Li S, Sinkins WG, Gosling M, Bahra P, Poll C, Westwick J & Schilling WP (2004). Activation of human TRPC6 channels by receptor stimulation. J Biol Chem 279, 22047-22056.
12. + during whole-cell recording. Biophys J 78, 1872-1880.
13. Garcia RL & Schilling WP (1997). Differential expression of mammalian Trp homologues across tissues and cell lines. Biochem Biophys Res Commun 239, 279-283.
14. + - ATPase. Pflugers Arch - Eur J Physiol 451, 87-98.
15. Goel M, Sinkins WG & Schilling WP (2002). Selective association of TRPC channel subunits in rat brain synaptosomes. J Biol Chem 277, 48303-48310.
16. 2+ indicators with improved fluorescence properties. J Biol Chem 260, 3440-3450.
17. Hassock SR, Zhu MX, Trost C, Flockerzi V & Authi KS (2002). Expression and role of TRPC proteins in human platelets: Evidence that TRPC6 forms the store-independent calcium entry channel. Blood 100, 2801-2811.
18. Hille B (2001). Selective permeability: Saturation and binding. In Ion Channels of Excitable Membrane. Hille B, pp. 471-502. Sinauer Associates, Inc., Massachusetts.
19. Hofmann T, Obukhov AG, Schaefer M, Harteneck C, Gudermann T & Schultz G (1999). Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature 397, 259-263.
20. Hofmann T, Schaefer M, Schultz G & Gudermann T (2002). Subunit composition of mammalian transient receptor potential channels in living cells. Proc Natl Acad Sci U S A 99, 7461-7466.
21. 2+-permeable cation channel. Circ Res 88, 325-332.
22. Jung S, Strotmann R, Schultz G & Plant TD (2002). TRPC6 is a candidate channel involved in receptor stimulated cation currents in A7r5 smooth muscle cells. Am J Physiol Cell Physiol 282, C347-C359.
23. Kamouchi M, Philipp S, Flockerzi V, Wissenbach U, Mamin A, Raeymaekers L, Eggermont J, Droogmans G & Nilius B (1999). Properties of heterologously expressed hTRP3 channels in bovine pulmonary artery endothelial cells. J Physiol 518, 345-358.
24. Korn SJ & Horn R (1989). Influence of sodium-calcium exchange on calcium current rundown and the duration of calcium-dependent chloride currents in pituitary cells studied with whole cell and perforated patch recording. J Gen Physiol 94, 789-812.
25. 2+ entry into HEK293 cells. Cell Signal 17, 437-445.
26. Montell C (2005). The TRP superfamily of cation channels. Sci STKE; DOI: 10.1126/stke.2722005re3.
27. 2+ block and regulates inward current amplitude of a transient receptor potential channel. J Neurosci 25, 1234-1239.
28. Okada T, Inoue R, Yamazaki K, Maeda A, Kurosaki T, Yamakuni T, Tanaka I, Shimizu S, Ikenaka K, Imoto K & Mori Y (1999). Molecular and functional characterization of a novel mouse transient receptor potential protein homologue TRP7. J Biol Chem 274, 27359-27370.
29. Reading SA, Earley S, Waldron BJ, Welsh DG & Brayden JE (2005). TRPC3 mediates pyrimidine receptor-induced depolarization of cerebral arteries. Am J Physiol Heart Circ Physiol 288, H2055-H2061.
30. Riccio A, Medhurst AD, Mattei C, Kelsell RE, Calver AR, Randall AD, Benham CD & Pannier B (2002). mRNA distribution analysis of human TRPC familly in CNS and peripheral tissues. Mol Brain Res 109, 95-104.
31. 2+ exchanger. J Biol Chem 279, 13696-13704.
32. Schilling WP (1989). Effect of membrane potential on cytosolic calcium of bovine aortic endothelial cells. Am J Physiol 257, H778-H784.
33. 2+-influx pathway. Biochem J 284, 521-530.
34. Schilling WP, Rajan L & Strobl-Jager E (1989). Characterization of the bradykinin-stimulated calcium influx pathway of cultured vascular endothelial cells: Saturability, selectivity and kinetics. J Biol Chem 264, 12838-12848.
35. Shi J, Mori E, Mori Y, Mori M, Li J, Ito Y & Inoue R (2004). Multiple regulation by calcium of murine homologues of transient receptor potential proteins TRPC6 and TRPC7 expressed in HEK293 cells. J Physiol 561, 415-432.
36. Smith RJ, Sam LM, Justen JM, Bundy GL, Bala GA & Bleasdale JE (1990). Receptor-coupled signal transduction in human polymorphonuclear neutrophils: Effects of a novel inhibitor of phospholipase C-dependent processes on cell responsiveness. J Pharmacol Exp Ther 253, 688-697.
37. 2+ signal generation in A7r5 smooth muscle cells. J Biol Chem 280, 39786-39794.
38. Strubing C, Krapivinsky G, Krapivinsky L & Clapham DE (2001). TRPC1 and TRPC5 form a novel cation channel in mammalian brain. Neuron 29, 645-655.
39. 2+ channel in PC12 cells (an adrenal chromaffin cell line) correlates with expression of the TRP-6 (transient receptor potential) protein. Biochem J 358, 717-726.
40. Thompson AK, Mostafapour SP, Denlinger LC, Bleasdale JE & Fisher SK (1991). The aminosteriod U-73122 inhibits muscarinic receptor sequestration and phosphoinositide hydrolysis in SK-N-SH neuroblastoma cells. J Biol Chem 266, 23856-23862.
41. Tseng P-H, Lin H-P, Hu H, Wang C, Zhu MX & Chen C-S (2004). The canonical transient receptor potential 6 channel as a putative phosphatidylinositol 3,4,5-trisphosphate-sensitive calcium entry system. Biochemistry 43, 11701-11708.
42. Welsh DG, Morielli AD, Nelson MT & Brayden JE (2002). Transient receptor potential channels regulate myogenic tone of resistance arteries. Circ Res 90, 248-250.
43. Yu Y, Sweeney M, Zhang S, Platoshyn O, Landsberg J, Rothman A & Yuan JXJ (2003). PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression. Am J Physiol Cell Physiol 284, C316-C330.
44. 2+ channel isoforms. J Biol Chem 276, 13331-13339.