Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells

Experimental Cell Research

Volumn 312, Issue 7, 2006, Pages 974-982

  Grunewald, Thomas G P ^a   Kammerer, Ulrike ^a   Schulze, Elfriede ^a   Schindler, Detlef ^a   Honig, Arnd ^a   Zimmer, Michael ^a   Butt, Elke ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

Breast cancer; BT 20; Cell cycle; Focal contacts; LASP 1; MCF 7; Metastasis; Migration; siRNA; Zyxin

Indexed keywords

ACTIN; LIM PROTEIN; PROTEIN SH3; SMALL INTERFERING RNA; ZYXIN;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CANCER INHIBITION; CELL CYCLE; CELL CYCLE G2 PHASE; CELL MIGRATION; CELL MOTILITY; CELL PROLIFERATION; CONTROLLED STUDY; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; STRESS FIBER; TUMOR CELL;

MINK CELL FOCUS-FORMING VIRUS;

EID: 33645214432     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.yexcr.2005.12.016     Document Type: Article

References (31)

1. A. Jemal, T. Murray, E. Ward, A. Samuels, R.C. Tiwari, A. Ghafoor, E.J. Feuer, and M.J. Thun Cancer statistics, 2005 CA Cancer J. Clin. 55 2005 10 30
2. P.A. Greenberg, G.N. Hortobagyi, T.L. Smith, L.D. Ziegler, D.K. Frye, and A.U. Buzdar Long-term follow-up of patients with complete remission following combination chemotherapy for metastatic breast cancer J. Clin. Oncol. 14 1996 2197 2205
3. C.K. Osborne Tamoxifen in the treatment of breast cancer N. Engl. J. Med. 339 1998 1609 1618
4. C. Tomasetto, C. Moog-Lutz, C.H. Regnier, V. Schreiber, P. Basset, and M.C. Rio LASP-1 (MLN 50) defines a new LIM protein subfamily characterized by the association of LIM and SH3 domains FEBS Lett. 373 1995 245 249
5. C. Tomasetto, C. Regnier, C. Moog-Lutz, M.G. Mattei, M.P. Chenard, R. Liderau, P. Basset, and M.C. Rio Identification of four novel human genes amplified and overexpressed in breast carcinoma and localized to the q11-q21.3 region of chromosome 17 Genomic 28 1995 367 376
6. V. Schreiber, C. Moog-Lutz, C.H. Regnier, M.P. Chenard, H. Boeuf, J.L. Vonesch, C. Tomasetto, and M.C. Rio LASP-1, a novel type of actin-binding protein accumulating in cell membrane extensions Mol. Med. 4 1998 675 687
7. S.C. Chew, X. Chen, J.A. Parente, S. Tarrer, C. Okamoto, and H.Y. Qin LASP-1 binds to non-muscle F-actin in vitro and is localized within multiple sites of dynamic actin assembly in vivo J. Cell Sci. 115 2002 4787 4799
8. E. Butt, S. Gambaryan, N. Göttfert, A. Galler, K. Marcus, and H.E. Meyer Actin binding of human LIM and SH3 protein is regulated by cAMP- and cGMP-dependent protein kinase phosphorylation on serine 146 J. Biol. Chem. 278 2003 15601 15607
9. C. Keicher, S. Gambaryan, E. Schulze, K. Marcus, H.E. Meyer, and E. Butt Phosphorylation of mouse LASP-1 on threonine 156 by cAMP- and cGMP-dependent protein kinase Biochem. Biophys. Res. Commun. 24 2004 308 316
10. B. Li, L.B. Zhuang, and B. Trueb Zyxin interacts with the SH3 domains of the cytoskeletal proteins LIM-nebulette and LASP-1 J. Biol. Chem. 279 2004 20401 20410
11. C.S. Chew, J.R. Parente, C.J. Zhou, E. Baranco, and X. Chen LASP-1 is a regulated phosphoprotein within the cAMP-signaling pathway in the gastric parietal cell J. Physiol. 275 1998 C56 C67
12. C.S. Chew, J.A. Parente, X. Chen, C. Chaponnier, and R.S. Cameron The LIM and SH3 domain containing protein, LASP-1, may link the cAMP-signaling pathway with dynamic membrane restructuring activities in ion transporting epithelia J. Cell Sci. 113 2000 2035 2045
13. H.Y. Lin, Z.Y. Park, D. Lin, A.A. Brahmbhatt, M. Rio, J.R. Yates, and R.L. Klemke Regulation of cell migration and survival by focal adhesion targeting of LASP-1 J. Cell Biol. 165 2004 421 432
14. K. Rottner, M. Krause, M. Gimona, J.V. Small, and J. Wehland Zyxin is not colocalized with VASP at lamellipodial Tipps and exhibits different dynamics to vinculin, paxillin and VASP in focal adhesions Mol. Biol. Cell 12 2000 3013 3103
15. D. Schindler, and H. Hoehn Flow cytometric testing for syndromes with chromosomal instability R.D. Wegner Diagnostic Cytogenetics 1999 Springer Berlin 269 281
16. M. Reinhard, T. Jarchau, and U. Walter Actin-bases motility. Stopp and go with Ena/VASP proteins Trends Biochem. Sci. 4 2001 243 249
17. C.R. Liu, C.S. Ma, J.Y. Ning, J.F. You, S.L. Liao, and J. Zheng Differential thymosin beta 10 expression levels and actin filament organisation in tumor cell lines with different metastatic potential Chin. Med. J. 117 2004 213 218
18. A.J. Ridley, M.A. Schwartz, K. Burridge, R.A. Firtel, M.H. Ginsberg, G. Borisy, J.T. Parsons, and A.R. Horwitz Cell migration: integrating signals from front to back Science 302 2003 1704 1709
19. I. Bieche, C. Tomasetto, H. Regnier, C. Moog-Lutz, M.C. Rio, and R. Liderau Two distinct amplified regions at 17q11-q21 involved in human primary breast cancer Cancer Res. 56 1996 3886 3890
20. G. Loughran, M. Huigsloot, P.A. Kiely, L.M. Smith, S. Floud, V. Ayllon, and R. O'connor Gene expression profiles in cells transformed by overexpression of the IGF-1 receptor Oncogene 24 2005 6185 6193
21. W.J. Ingram, C.A. Wicking, S.M. Grimmond, A.R. Forrest, and B.J. Wainwright Novel genes regulated by sonic Hedgehog in pluripotent mesenchymal cells Oncogene 21 2002 8196 8205
22. Y. Wang, I.V. Tetko, M.A. Hall, E. Frank, A. Facius, K.F. Mayer, and H.W. Mewes Gene selection from microarray data for cancer classification-A machine learning approach Comput. Biol. Chem. 29 2005 37 46
23. M.C. Beckerle Zyxin: zinc fingers at sites of cell adhesion Bioassays 19 1997 949 957
24. E. Griffith, A.S. Coutts, and D.M. Black RNAi knock-down of the focal adhesion protein TES reveals its role in actin stress fibre organisation Cell Motil. Cytoskelet. 60 2005 140 152
25. M. Cattaruzza, C. Lattrich, and M. Hecker Focal adhesion protein zyxin is a mechanosensitive modulator of gene expression in vascular muscle cells Hypertension 43 2004 726 730
26. B.E. Drees, K.M. Andrews, and M.C. Beckerle Molecular dissection of zyxin function reveals its involvement in cell motility J. Cell Biol. 147 1999 1549 1560
27. D.A. Nix, J. Fradelizi, S. Bockholt, B. Menichi, D. Louvard, E. Friedrich, and M.C. Becherle Targeting of zyxin to sites of actin membrane interaction and to nucleus J. Biol. Chem. 276 2001 34759 34767
28. Y. Wang, and T.D. Gilmore Zyxin and Paxillin proteins: focal adhesion plaque LIM domain proteins go nuclear Biochim. Biophys. Acta 1593 2003 115 120
29. J.L. Kadrmas, and M.C. Beckerle The LIM domain: from the cytoskeleton to the nucleus Nat. Rev., Mol. Cell Biol. 5 2004 920 931
30. T. Hirota, T. Morisaki, Y. Nishiyama, T. Marumoto, K. Tada, T. Hara, N. Masuko, M. Inagaki, K. Hatakeyama, and H. Saya Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor J. Cell Biol. 5 2000 1073 1086
31. S. Iida, T. Hirota, T. Morisaki, T. Marumoto, T. Hara, S. Kuninaka, S. Honda, K. Kosai, M. Kawasuji, D.C. Pallas, and H. Saya Tumor suppressor WARTS ensures genomic integrity by regulating both mitotic progression and tetraploidy checkpoint function Oncogene 23 2004 5266 5274