Nested multigene MSP/DHPLC method for analyzing promoter hypermethylation status in clinical samples

BioTechniques

Volumn 40, Issue 1, 2006, Pages 40-48

  Divine, Kevin K ^a   Liechty, Kieu C ^a   Crume, Kevin C ^a   Belinsky, Steven A ^a  

^a LOVELACE RESPIRATORY RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEATH ASSOCIATED PROTEIN KINASE; PROTEIN P16; RAS ASSOCIATION DOMAIN FAMILY PROTEIN 1A; APOPTOSIS REGULATORY PROTEIN; DNA; PROTEIN KINASE (CALCIUM,CALMODULIN);

AGAR GEL ELECTROPHORESIS; ANALYTIC METHOD; CONTROLLED STUDY; DENATURING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; DNA METHYLATION; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; MULTIGENE FAMILY; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; REVIEW; TUMOR SUPPRESSOR GENE; ARTICLE; BREAST TUMOR; COMPARATIVE STUDY; CPG ISLAND; EVALUATION; FEMALE; GENE SILENCING; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSTRUMENTATION; LUNG TUMOR; METABOLISM; METHODOLOGY; NEOPLASM; SMOKING;

APOPTOSIS REGULATORY PROTEINS; BREAST NEOPLASMS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CPG ISLANDS; DNA; DNA METHYLATION; DNA, NEOPLASM; FEMALE; GENE SILENCING; GENES, P16; GENES, TUMOR SUPPRESSOR; HUMANS; LUNG NEOPLASMS; NEOPLASMS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); SMOKING;

EID: 33644939972     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112095     Document Type: Review

References (12)

1. Jones, P.A. and S.B. Baylin. 2002. The fundamental role of epigenetic events in cancer. Nat. Rev. Genet. 3:415-428.
2. Esteller, M. and J.G. Herman. 2002. Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumours. J. Pathol. 196:1-7.
3. Belinsky, S.A. 2004. Gene-promoter hypermethylation as a biomarker in lung cancer. Nat. Rev. Cancer 4:707-717.
4. Herman, J.G., J.R. Graff, S. Myöhänen, B.D. Nelkin, and S.B. Baylin. 1996. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. USA 93:9821-9826.
5. Palmisano, W.A., K.K. Divine, G. Saccomanno, F.D. Gilliland, S.B. Baylin, J.G. Herman, and S.A. Belinsky. 2000. Predicting lung cancer by detecting aberrant promoter methylation in sputum. Cancer Res. 60:5954-5958.
6. Belinsky, S.A., D.M. Klinge, J.P. Dekker, M.W. Smith, T.J. Bocklage, F.D. Gilliland, R.E. Crowell, D.D. Karp, et al. 2005. Gene promoter methylation in plasma and sputum increases with lung cancer risk. Clin. Cancer Res. 11:6505-6511.
7. Fackler, M.J., M. McVeigh, J. Mehrotra, M. A. Blum, J. Lange, Lapides, E. Garrett, P. Argani, and S. Sukumar. 2004. Quantitative multiplex methylation-specific PCR assay for the detection of promoter hypermethylation in multiple genes in breast cancer. Cancer Res. 64:4442-4452.
8. Divine, K.K., L.C. Pulling, P.G. Marron-Terada, K.C. Liechty, T. Kang, A.G. Schwartz, T.J. Bocklage, T.A. Coons, et al. 2005. Multiplicity of abnormal promoter methylation in lung adenocarcinomas from smokers and never smokers. Int. J. Cancer 114:400-405.
9. Esteller, M., P.G. Corn, S.B. Baylin, and J.G. Herman. 2001. A gene hypermethylation profile of human cancer. Cancer Res. 61:3225-3229.
10. Palmisano, W.A., K.P. Crume, M.J. Grimes, S.A. Winters, M. Toyota, M. Esteller, N. Joste, S.B. Baylin, and S.A. Belinsky. 2003. Aberrant promoter methylation of the transcription factor genes PAX5α and β in human cancers. Cancer Res. 63:4620-4625.
11. Akiyama, Y., N. Watkins, H. Suzuki, K.W. Jair, M. van Engeland, M. Esteller, H. Sakai, C. Ren, et al. 2003. GATA-4 and GATA-5 transcription factor genes and potential downstream antitumor target genes are epigenetically silenced in colorectal and gastric cancer. Mol. Cell. Biol. 23:8429-8439.
12. Guo, M., Y. Akiyama, M.G. House, C.M. Hooker, E. Heath, E. Gabrielson, S.C. Yang, Y. Han, et al. 2004. Hypermethylation of the GATA genes in lung cancer. Clin. Cancer Res. 10:7917-7924.