A simple correction for cell autofluorescence for multiparameter cell-based analysis of human solid tumors

Cytometry Part B - Clinical Cytometry

Volumn 70, Issue 2, 2006, Pages 91-103

  Smith, Charles A ^a   Pollice, Agnese ^a   Emlet, David ^a   Shackney, Stanley E ^a  

^a ALLEGHENY GENERAL HOSPITAL   (United States)

Author keywords

Autofluorescence; Cell based measurements; Immunofluorescence; Solid tumors

Indexed keywords

DNA; EPIDERMAL GROWTH FACTOR RECEPTOR 2; FLUOROCHROME;

ARGON LASER; ARTICLE; AUTOFLUORESCENCE; BREAST CANCER; CANCER CELL CULTURE; CYTOMETRY; DIODE LASER; HELIUM NEON LASER; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; LASER SCANNING CYTOMETRY; LUNG CANCER; MEASUREMENT; PRIORITY JOURNAL; SOLID TUMOR;

BREAST NEOPLASMS; CELL LINE, TUMOR; COMPUTER SIMULATION; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; HUMANS; LASER SCANNING CYTOMETRY; LUNG NEOPLASMS; RECEPTOR, ERBB-2;

EID: 33644916094     PISSN: 15524949     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.b.20090     Document Type: Article

References (19)

1. Corsetti JP, Sotirchos SV, Cox C, Cowles JW, Leary JF, Blumburg N. Correction of cellular autofluorescence in flow cytometry by mathematical modeling of cellular fluorescence. Cytometry 1988;9:539-547.
2. Steinkamp JA, Lehnert NM, Keij JF, Lehnert BE. Enhanced immunofluorescence measurement resolution of surface antigens on highly autofluorescent, glutaraldehyde-fixed cells analyzed by phase-sensitive flow cytometry. Cytometry 1999;37:275-283.
3. Tadrous PJ, Siegel J, French PM, Shousha S, Lalani el-N, Stamp GW. Fluorescence lifetime imaging of unstained tissues: early results in human breast cancer. J Pathol 2003;199:309-317.
4. Steinkamp JA, Stewart CC. Dual-laser, differential fluorescence correction method for reducing cellular background autofluorescence. Cytometry 1986;7:566-574.
5. Roederer M, Murphy RF. Cell-by-cell autofluorescence correction for low signal-to-noise systems: application to epidermal growth factor endocytosis by 3T3 fibroblasts. Cytometry 1986;7:558-565.
6. Alberti S, Parks DR, Herzenberg LA. A single laser method for subtraction of cell autofluorescence in flow cytometry. Cytometry 1987;8:114-119.
7. Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T. Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J Histochem Cytochem 2000;48:653-662.
8. Macville MV, Van der Laak JA, Speel EJ, Katzir N, Garini Y, Soenksen D, McNamara G, de Wilde PC, Hanselaar AG, Hopman AH, Ried T. Spectral imaging of multi-color chromogenic dyes in pathological specimens. Anal Cell Pathol 2001;22:133-142.
9. Ecker RC, de Martin R, Steiner GE, Schmid JA. Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis. Cytometry A 2004;59:172-181.
10. Mosiman VL, Patterson BK, Canterero L, Goolsby CL. Reducing cellular autofluorescence in flow cytometry: an in situ method. Cytometry 1997;30:151-156.
11. Hallden G, Skold CM, Eklund A, Forslid J, Hed J. Quenching of intracellular autofluorescence in alveolar macrophages permits analysis of fluorochrome labelled surface antigens by flow cytofluorometry. J Immunol Methods 1991;142:207-214.
12. Hodge SJ, Hodge GL, Holmes M, Reynolds PN. Flow cytometric characterization of cell populations in bronchoalveolar lavage and bronchial brushings from patients with chronic obstructive pulmonary disease. Cytometry B Clin Cytom 2004;61:27-34.
13. Kunz WS. Spectral properties of fluorescent flavoproteins of isolated rat liver mitochondria. FEBS Lett 1986;195:92-96.
14. Heintzelman DL, Lotan R, Richards-Kortum RR. Characterization of the autofluorescence of polymorphonuclear leukocytes, mononuclear leukocytes and cervical epithelial cancer cells for improved spectroscopic discrimination of inflammation from dysplasia. Photochem Photobiol 2000;71:327-332.
15. Palmer GM, Keely PJ, Breslin TM, Ramanujam N. Autofluorescence spectroscopy of normal and malignant human breast cell lines. Photochem Photobiol 2003;78:462-469.
16. Kindzelskii A, Petty HR. Fluorescence spectroscopic detection of mitochondrial flavoprotein redox oscillations and transient reduction of the NADPH oxidase-associated flavoprotein in leukocytes. Eur Biophys J 2004;33:291-299.
17. Pollice A, McCoy JP, Shackney S, Smith C, Agarwal J, Burholt D, Janocko L, Hornicek F, Singh S Hartsock R. Sequential paraformaldehyde and methanol fixation for simultaneous flow cytometric analysis of DNA, cell surface proteins, and intracellular proteins. Cytometry 1992;13:432-444.
18. Shackney SE, Smith CA, Pollice AA, Brown K, Kosiban D. A suitable method for identifying cell aggregates in laser scanning cytometry listmode data for analyzing disaggregated cell suspensions obtained from human cancers. Cytometry 2004;59B:10-23.
19. Shackney SE, Shankey TV. Common patterns of genetic evolution in human solid tumors. Cytometry 1997;29:1-27.