Cloning and functional analysis of a high DP fructan:fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): Comparison of the native and recombinant enzymes

Journal of Experimental Botany

Volumn 57, Issue 4, 2006, Pages 775-789

  Van Den Ende, Wim ^a   Clerens, Stefan ^a   Vergauwen, Rudy ^a   Boogaerts, David ^a   Le Roy, Katrien ^a   Arckens, Lutgarde ^a   Van Laere, André ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

1 FFT; Biotechnology; Echinops ritro; Fructan; Heterologous expression; Inulin; Pichia pastoris

Indexed keywords

BIOREACTORS; BIOTECHNOLOGY; CLONING; DNA; FUNCTIONAL ASSESSMENT; GENES; PLANTS (BOTANY); POLYMERIZATION;

1-FFT; ASTERACEAE; DEGREE OF POLYMERIZATION (DP); ECHINOPS RITRO; FRUCTAN; FRUCTO-OLIGOSACCHARIDES (FOS); HETEROLOGOUS EXPRESSION; INULIN; PICHIA PASTORIS;

ENZYMES;

1,2 BETA FRUCTAN 1(F) FRUCTOSYLTRANSFERASE; 1,2-BETA-FRUCTAN 1(F)-FRUCTOSYLTRANSFERASE; COMPLEMENTARY DNA; FRUCTAN; GLYCOSYLTRANSFERASE; HYBRID PROTEIN;

ACCLIMATIZATION; AMINO ACID SEQUENCE; ARTICLE; ASTERACEAE; BIOSYNTHESIS; CHEMISTRY; COMPARATIVE STUDY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; MASS SPECTROMETRY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PHYLOGENY; SEQUENCE ALIGNMENT;

ACCLIMATIZATION; AMINO ACID SEQUENCE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ECHINOPS PLANT; FRUCTANS; HEXOSYLTRANSFERASES; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PHYLOGENY; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

ASTERACEAE; CICHORIUM INTYBUS; CIRSIUM; CYNARA SCOLYMUS; DAHLIA; DAHLIA VARIABILIS; ECHINOPS RITRO; HELIANTHUS TUBEROSUS; PICHIA PASTORIS; VIGUIERA DISCOLOR;

EID: 33644902979     PISSN: 00220957     EISSN: 14602431     Source Type: Journal    
DOI: 10.1093/jxb/erj065     Document Type: Article

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