Development of a one step real-time RT-PCR method for sensitive detection of human astrovirus

Journal of Virological Methods

Volumn 133, Issue 1, 2006, Pages 14-19

  Royuela, Enrique ^a   Negredo, Ana ^a   Sánchez Fauquier, Alicia ^a  

^a INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

Human astrovirus (HAstV); Molecular detection; One step; Real time RT PCR; SYBR green

Indexed keywords

ARTICLE; ASTROVIRUS; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; HUMAN; INFANT; NEWBORN; NONHUMAN; PRESCHOOL CHILD; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPE; VALIDATION PROCESS; VIRUS DETECTION; VIRUS LOAD;

ACUTE DISEASE; ASTROVIRIDAE INFECTIONS; CHILD, PRESCHOOL; FEASIBILITY STUDIES; FLUORESCENT DYES; GASTROENTERITIS; HUMANS; INCIDENCE; INDICATOR DILUTION TECHNIQUES; MAMASTROVIRUS; NUCLEIC ACID DENATURATION; ORGANIC CHEMICALS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEROEPIDEMIOLOGIC STUDIES; SEROTYPING; TEMPERATURE;

ASTROVIRIDAE; HUMAN ASTROVIRUS;

EID: 33644893883     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2005.10.012     Document Type: Article

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