Development of an astrovirus RT-PCR detection assay for use with conventional, real-time, and integrated cell culture/RT-PCR

Canadian Journal of Microbiology

Volumn 50, Issue 4, 2004, Pages 269-278

  Grimm, Ann C ^a   Cashdollar, Jennifer L ^a   Williams, Frederick P ^a   Fout, G Shay ^a  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

Author keywords

Astrovirus; Environmental water; ICC RT PCR; Real time PCR; RT PCR

Indexed keywords

CELLS; DISEASES; ENZYMES; GASTROENTEROLOGY; MICROBIOLOGY; OPTIMIZATION; SENSITIVITY ANALYSIS;

ASTROVIRUSES; ENVIRONMENTAL WATER; GASTROENTERITIS;

VIRUSES;

DETECTION METHOD; POLYMERASE CHAIN REACTION; REAL TIME; VIRAL DISEASE; WATERBORNE DISEASE;

ANALYTIC METHOD; ARTICLE; ASTROVIRUS; CELL CULTURE; CELL STRAIN CACO 2; FECES ANALYSIS; INCUBATION TIME; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SEROTYPE; VIRUS DETECTION; WATER SAMPLING;

CACO-2 CELLS; CELLS, CULTURED; HUMANS; MAMASTROVIRUS; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

ASTROVIRIDAE; RNA VIRUSES;

EID: 3142523347     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w04-012     Document Type: Article

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