The malate dehydrogenase isoforms from Trypanosoma brucei: Subcellular localization and differential expression in bloodstream and procyclic forms

International Journal for Parasitology

Volumn 36, Issue 3, 2006, Pages 295-307

  Aranda, Alejandro ^a   Maugeri, Dante ^b   Uttaro, Antonio D ^c   Opperdoes, Fred ^d   Cazzulo, Juan J ^b   Nowicki, C ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b INSTITUTO DE INVESTIGACIONES BIOTECNOLÓGICAS   (Argentina)

^c UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

^d DE DUVE INSTITUTE   (Belgium)

Author keywords

Isozymes; Malate dehydrogenase; Trypanosoma brucei

Indexed keywords

ISOENZYME; MALATE DEHYDROGENASE; OXALOACETIC ACID; RABBIT ANTISERUM; RECOMBINANT ENZYME;

ENZYME;

ANIMAL EXPERIMENT; ARTICLE; CELLULAR DISTRIBUTION; CHROMATOGRAPHY; CONTROLLED STUDY; CROSS REACTION; ENZYME BLOOD LEVEL; ENZYME LOCALIZATION; ENZYME MECHANISM; ENZYME REGULATION; ESCHERICHIA COLI; LEISHMANIA MAJOR; LIFE CYCLE; MALE; MOLECULAR CLONING; MOLECULAR EVOLUTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITOSIS; PHYLOGENETIC TREE; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; REDUCTION; SYNTENY; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, AGAROSE; CROSS REACTIONS; CYTOSOL; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENES, PROTOZOAN; ISOENZYMES; MALATE DEHYDROGENASE; MICROBODIES; MITOCHONDRIA; OXALOACETATE; PHENYLPYRUVIC ACIDS; PHYLOGENY; PROTOZOAN PROTEINS; RABBITS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; TRYPANOSOMA BRUCEI BRUCEI;

ANIMALIA; ESCHERICHIA COLI; LEISHMANIA MAJOR; ORYCTOLAGUS CUNICULUS; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI;

EID: 33644858560     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpara.2005.09.013     Document Type: Article

References (37)

1. S.A. Anderson, V. Carter, C.B. Hagen, and M. Parsons Molecular cloning of the glycosomal malate dehydrogenase of Trypanosoma brucei Mol. Biochem. Parasitol. 96 1998 185 189
2. S.A. Anderson, V. Carter, and M. Parsons Trypanosoma brucei: molecular cloning and stage-regulated expression of a malate dehydrogenase localized to the mitochondrion Exp. Parasitol. 89 1998 63 70
3. S. Besteiro, M.P. Barrett, L. Riviere, and F. Bringaud Energy generation in insect stages of Trypanosoma brucei: metabolism in flux Trends Parasitol. 21 2005 185 191
4. H. Blum, H. Beier, and H.J. Gross Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels Electrophoresis. 8 1987 93 99
5. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
6. J.J. Cannata, and J.J. Cazzulo Glycosomal and mitochondrial malate dehydrogenases in epimastigotes of Trypanosoma cruzi Mol. Biochem. Parasitol. 11 1984 37 49
7. J.J. Cannata, A.C. Frasch, M.A. Cataldi de Flombaum, E.L. Segura, and J.J. Cazzulo Two forms of 'malic' enzyme with different regulatory properties in Trypanosoma cruzi Biochem. J. 184 1979 409 419
8. J.J. Cazzulo Aerobic fermentation of glucose by trypanosomatids Fed. Am. Soc. Exp. Biol. J. 6 1992 3153 3161
9. M.C. Cazzulo-Franke, J. Vernal, J.J. Cazzulo, and C. Nowicki The NAD-linked aromatic alpha-hydroxy acid dehydrogenase from Trypanosoma cruzi. A new member of the cytosolic malate dehydrogenases group without malate dehydrogenase activity Eur. J. Biochem. 266 1999 903 910
10. V. Costou, S. Besteiro, L. Rivière, M. Birau, N. Biteau, J-M. Franconi, M. Boshart, T. Baltz, and F. Bringaud A mitochondrial NADH-dependant fumarate reductase involved in the production of succinate excreted by procyclic Trypanosoma brucei J. Biol. Chem. 280 2005 16559 16570
11. A. Denicola, H. Rubbo, L. Haden, and J.F. Turrens Extramitochondrial localization of NADH-fumarate reductase in trypanosomatids Comp Biochem. Physiol. B Biochem. Mol. Biol. 133 2002 23 27
12. W. Eventoff, M.G. Rossman, S.S. Taylor, H.J. Torff, H. Meyer, W. Keil, and H.H. Kiltz Structural adaptations of lactate dehydrogenase isoenzymes Proc. Natl Acad. Sci. USA 74 1977 2677 2681
13. Fraser, R.D.B., Suzuki, E., 1973. The use of least squares in the data analysis. In: Leach, S.J. (Ed), Physic. Princ. Tech. Protein. Chem. 21, 301-355.
14. C. Gietl Malate dehydrogenase isoenzymes: cellular locations and role in the flow of metabolites between the cytoplasm and cell organelles Biochim. Biophys. Acta 1100 1992 217 234
15. F. Glaser, T. Pupko, I. Paz, R.E. Bell, D. Bechor-Shental, E. Martz, and N. Ben-Tal ConSurf: identification of functional regions in proteins by surface-mapping of phylogenetic information Bioinformatics 19 2003 163 164
16. C.R. Goward, and D.J. Nicholls Malate dehydrogenase: a model for structure, evolution, and catalysis Protein Sci. 3 1994 1883 1888
17. V. Hannaert, M.A. Albert, D.J. Rigden, M.T. da Silva Giotto, O. Thiemann, R.C. Garratt, J. Van Roy, F.R. Opperdoes, and P.A. Michels Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolase Eur. J. Biochem. 270 2003 3205 3213
18. V. Hannaert, F. Bringaud, F.R. Opperdoes, and P.A. Michels Evolution of energy metabolism and its compartmentation. Kinetoplastida Kinetoplastid Biol. Dis. Biomed. Central 2 2003 11 30
19. D.T. Hart, O. Misset, S.W. Edwards, and F.R. Opperdoes A comparison of the glycosomes (microbodies) isolated from Trypanosoma brucei bloodstream form and cultured procyclic trypomastigotes Mol. Biochem. Parasitol. 12 1984 25 35
20. G.R. Hunter, U. Hellman, J.J. Cazzulo, and C. Nowicki Tetrameric and dimeric malate dehydrogenase isoenzymes in Trypanosoma cruzi epimastigotes Mol. Biochem. Parasitol. 105 2000 203 214
21. M. Kimura A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences J. Mol. Evol. 16 1980 111 120
22. S.M. Lanham, and D.G. Godfrey Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose Exp. Parasitol. 28 1970 521 534
23. E. Medina-Acosta, and G.A. Cross Rapid isolation of DNA from trypanosomatid protozoa using a simple 'mini-prep' procedure Mol. Biochem. Parasitol. 59 1993 327 329
24. P.A. Michels, V. Hannaert, and F. Bringaud Metabolic aspects of glycosomes in trypanosomatidae - new data and views Parasitol. Today 16 2000 482 489
25. M. Montemartini, J.A. Santomé, J.J. Cazzulo, and C. Nowicki Purification and partial structural and kinetic characterization of an aromatic l-α-hydroxy acid dehydrogenase from epimastigotes of Trypanosoma cruzi Mol. Biochem. Parasitol. 68 1994 15 23
26. F.R. Opperdoes Compartmentation of carbohydrate metabolism in trypanosomes Annu. Rev. Microbiol. 41 1987 127 151
27. F.R. Opperdoes, P. Borst, and H. Spits Particle-bound enzymes in the bloodstream form of Trypanosoma brucei Eur. J. Biochem. 76 1977 211 228
28. K.S. Paul, D. Jiang, Y.S. Morita, and P.T. Englund Fatty acid synthesis in African trypanosomes: a solution to the myristate mystery Trends Parasitol. 17 2001 381 387
29. H. Schägger, and G. von Jagow Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa Anal. Biochem. 166 1987 368 379
30. K. Strimmer, and A. von Haeseler Likelihood-mapping: a simple method to visualize phylogenetic content of a sequence alignment Proc. Natl Acad. Sci. USA 94 1997 6815 6819
31. J.D. Thompson, D.G. Higgins, and T.J. Gibson CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acid Res. 22 1994 4673 4680
32. A.D. Uttaro, S.G. Altabe, M.H. Rider, P.A. Michels, and F.R. Opperdoes A family of highly conserved glycosomal 2-hydroxyacid dehydrogenases from Phytomonas sp J. Biol. Chem. 275 2000 31833 31837
33. J.J. van Hellemond, F.R. Opperdoes, and A.G. Tielens Trypanosomatidae produce acetate via a mitochondrial acetate:succinate CoA transferase Proc. Natl Acad. Sci. USA 95 1998 3036 3041
34. S.W. van Weelden, B. Fast, A. Vogt, P. van der Meer, J. Saas, J.J. van Hellemond, A.G. Tielens, and M. Boshart Procyclic Trypanosoma brucei do not use Krebs cycle activity for energy generation J. Biol. Chem. 278 2003 12854 12863
35. S.W. van Weelden, J.J. van Hellemond, F.R. Opperdoes, and A.G. Tielens New functions of parts of the Krebs cycle in procyclic Trypanosoma brucei, a cycle not operating as a cycle J. Biol. Chem. 280 2005 12451 12460
36. E. Vassella, K. Straesser, and M.A. Boshart Mitochondrion-specific dye for multicolor fluorescent imaging of Trypanosoma brucei Mol. Biochem. Parasitol. 90 1997 381 385
37. J. Vernal, J. Muñoz-Jordan, M. Muller, J.J. Cazzulo, and C. Nowicki Sequencing and heterologous expression of a cytosolic-type malate dehydrogenase of Trypanosoma brucei Mol. Biochem. Parasitol. 117 2001 217 221