Trypanosoma brucei: Molecular cloning and stage-regulated expression of a malate dehydrogenase localized to the mitochondrion

Experimental Parasitology

Volumn 89, Issue 1, 1998, Pages 63-70

  Anderson, Steven A ^a,b   Carter, Victoria ^a   Parsons, Marilyn ^a,b  

^a SEATTLE BIOMEDICAL RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; ISOENZYME; MALATE DEHYDROGENASE;

ARTICLE; CARBOHYDRATE METABOLISM; CELLULAR DISTRIBUTION; ENERGY METABOLISM; ENZYME ACTIVITY; GENE EXPRESSION; MOLECULAR CLONING; PRIORITY JOURNAL; TRYPANOSOMA BRUCEI;

INSECTA; PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0032078501     PISSN: 00144894     EISSN: None     Source Type: Journal    
DOI: 10.1006/expr.1998.4260     Document Type: Article

References (30)

1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. 1990. Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410.
2. Banaszak, L. J., and Bradshaw, R. A. 1975. Malate dehydrogenases. In "The Enzymes" (P. D. Boyer, Ed.), Vol. 11a, pp. 369-396. Academic Press, New York.
3. Bienen, E. J., Saric, M., Pollakis, G., Grady, R. W., and Clarkson, A. B., Jr. 1991. Mitochondrial development in Trypanosoma brucei brucei transitional bloodstream forms. Molecular and Biochemical Parasitology, 45, 185-192.
4. Durieux, P.O., Schütz, P., Brun, R., and Köhler, P. 1991. Alterations in Krebs cycle enzyme activities and carbohydrate catabolism in two strains of Trypanosoma brucei during in vitro differentiation of their bloodstream to procyclic stages. Molecular and Biochemical Parasitology, 45, 19-28.
5. Effron, P. N., Torri, A. F., Engman, D. M., Donelson, J. E., and Englund, P. T. 1993. A mitochondrial heat shock protein from Crithidia fasciculata. Molecular and Biochemical Parasitology, 59, 191-200.
6. Evan, G. I., Lewis, G. K., Ramsay, G., and Bishop, J. M. 1985. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Molecular and Cellular Biology, 5, 3610-3616.
7. Fairlamb, A. H., and Opperdoes, F. R. 1986. Carbohydrate metabolism in African trypanosomes, with special reference to the glycosome. In "Carbohydrate Metabolism in Cultured Cells" (M. J. Morgan, Ed.), pp. 183-224. Plenum, New York.
8. Gietl, C. 1990. Glyoxysomal malate dehydrogenase from watermelon is synthesized with an amino-terminal transit peptide. Proceedings of the National Academy of Sciences USA, 87, 5773-5777.
9. Gietl, C., Lehnerer, M., and Olsen, O. 1990. Mitochondrial malate dehydrogenase from watermelon: Sequence of cDNA clones and primary structure of the higher-plant precursor protein. Plant Molecular Biology, 14, 1019-1030.
10. Gietl, C. 1992. Malate dehydrogenase isoenzymes: Cellular locations and role in the flow of metabolites between the cytoplasm and cell organelles. Biochimica et Biophysica Acta BioEnergetics, 1100,217-234.
11. Häuksler, T., Stierhof, Y. D., Blattner, J., and Clayton, C. 1997. Conservation of mitochondrial targeting sequence function in mitochondrial and hydrogenosomal proteins from the early-branching eukaryotes Crithidia, Trypanosoma and Trichomonas. European Journal of Cell Biology 73, 240-251.
12. Henikoff, S. 1987. Unidirectional digestion with exonuclease III in DNA sequence analysis. Methods in Enzymology, 155, 156-165.
13. McAlister-Henn, L., Blaber, M., Bradshaw, R. A., and Nisco, S. J. 1987. Complete nucleotide sequence of Escherichia coli gene encoding malate dehydrogenase. Nucleic Acids Research, 15, 4993
14. McAlister-Henn, L. 1988. Evolutionary relationships among the malate dehydrogenases. Trends in Biochemical Sciences, 13, 178-181.
15. Opperdoes, F.R., Borst, P., Bakker, S., and Leene, W. 1977. Localization of glycerol-3-phosphate oxidase in the mitochondrion and particulate NAD+-linked glycerol-3-phosphate dehydrogenase in the microbodies of the bloodstream form of Trypanosoma brucei. European Journal of Biochemistry, 76, 29-39.
16. Opperdoes, F.R., Markos, A., and Steiger, R. F. 1981. Localization of malate dehydrogenase, adenylate kinase, and glycolytic enzymes in glycosomes and the threonine pathway in the mitochondrion of culture procyclic trypomastigotes of Trypanosoma brucei. Molecular and Biochemical Parasitology, 4, 291-309.
17. Parker, H.L., Hill, T., Alexander, K., Murphy, N. B., Fish, W.R., and Parsons, M. 1995. Three genes and two isozymes: Gene conversion and the compartmentalization and expression of the phosphoglycerate kinases of Trypanosoma (Nannomonas) congolense. Molecular and Biochemical Parasitology, 69, 269-279.
18. Parsons, M., Stuart, K., and Smiley, B.L. 1991. Trypanosoma brucei: Analysis of codon usage and nucleotide composition of nuclear genes. Experimental Parasitology, 73, 101-105.
19. Parsons, M., and Hill, T. 1989. Elevated phosphoglycerate kinase mRNA but not protein in monomorphic Trypanosoma brucei: Implications for stage-regulation and post-transcriptional control. Molecular and Biochemical Parasitology, 33, 215-228.
20. Parsons, M., and Nielsen, B. 1990. Trypanosoma brucei: Two-dimensional gel analysis of the major glycosomal proteins during the life cycle. Experimental Parasitology, 70, 276-285.
21. Peterson, G. C., Sommer, J. M., Klosterman, S., Wang, C.C., and Parsons, M. 1997. Trypanosoma brucei: Identification of an internal region of phosphoglycerate kinase required for targeting to glycosomal microbodies. Experimental Parasitology, 85, 16-23.
22. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. "Molecular Cloning: A Laboratory Manual." Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Sommer, J.M., Cheng, Q.-L., Keller, G.-A., and Wang, C.C. 1992. In vivo import of firefly luciferase into the glycosomes of Trypanosoma brucei and mutational analysis of the C-terminal targeting signal. Molecular Biology of the Cell, 3, 749-759.
24. Torri, A. F., Bertrand, K.I., and Hajduk, S.L. 1993. Protein stability regulates the expression of cytochrome c during the developmental cycle of Trypanosoma brucei. Molecular and Biochemical Parasitology, 57, 305-316.
25. Torri, A. F., and Hajduk, S. L. 1988. Posttranscriptional regulation of cytochrome c expression during the developmental cycle of Trypanosoma brucei. Molecular and Cellular Biology, 8, 4625-4633.
26. Turrens, J. F. 1991. Mitochondrial metabolism of African trypanosomes. In "Biochemical Protozoology" (G.H. Coombs and M.J. North, Eds.), pp. 145-153.Taylor & Francis, Washington DC.
27. Verner, K. 1993. Co-translational protein import into mitochondria: An alternative view. Trends in Biochemical Sciences, 18, 366-371.
28. Vickerman, K. 1965. Polymorphism and mitochondrial activity in sleeping sickness trypanosomes. Nature, 208, 762-766.
29. Vogel, R.F., Entian, K.D., and Mecke, D. 1987. Cloning and sequence of the mdh structural gene of Escherichia coli. Archives of Microbiology, 149, 36-42.
30. von Heijne, G. 1986. Mitochondrial targeting sequences may form amphiphilic helices. The EMBO Journal, 5, 1335-1342.