Sequencing and heterologous expression of a cytosolic-type malate dehydrogenase of Trypanosoma brucei

Molecular and Biochemical Parasitology

Volumn 117, Issue 2, 2001, Pages 217-221

  Vernal, Javier ^a   Muoz Jordán, Jorge ^b   Müller, Miklós ^b   José Cazzulo, Juan ^c   Nowicki, Cristina ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b ROCKEFELLER UNIVERSITY   (United States)

^c INSTITUTO DE INVESTIGACIONES BIOTECNOLÓGICAS   (Argentina)

Author keywords

Heterologous expression; Malate dehydrogenase; Trypanosoma brucei

Indexed keywords

2 HYDROXYACID DEHYDROGENASE; AMINO ACID; DNA; ISOENZYME; MALATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; OXIDOREDUCTASE; RNA; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BLOOD FLOW; CELL ORGANELLE; DNA SEQUENCE; ENZYME LOCALIZATION; ENZYME MECHANISM; FLAGELLATE; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC CODE; GLYCOLYSIS; IMMUNOREACTIVITY; METABOLISM; MITOCHONDRION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI; TRYPOMASTIGOTE;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; CYTOSOL; ESCHERICHIA COLI; MALATE DEHYDROGENASE; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA; TRYPANOSOMA BRUCEI BRUCEI;

MASTIGOPHORA (FLAGELLATES); PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI;

EID: 0034793228     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(01)00343-7     Document Type: Article

References (20)

1. Malate dehydrogenase isoenzymes: Cellular locations and role in the flow of metabolites between the cytoplasm and cell organelles
2. Glycosomal and mitochondrial malate dehydrogenases in epimastigotes of Trypanosoma cruzi
3. Tetrameric and dimeric malate dehydrogenase isozymes in Trypanosoma cruzi epimastigotes
4. Trypanosoma brucei: Molecular cloning and stage-regulated expression of a malate dehydrogenase localized in the mitochondrion
5. Molecular cloning of the glycosomal malate dehydrogenase of Trypanosoma brucei
6. Compartmentation of carbohydrate metabolism in trypanosomes
7. The NAD linked aromatic α-hydroxy acid dehydrogenase from Trypanosoma cruzi. A new member of the cytosolic malate dehydrogenases group without malate dehydrogenase activity
8. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
9. Kinetic studies on the mechanism of the malate dehydrogenase reaction
10. Convergent evolution of Trichomonas vaginalis lactate dehydrogenase from malate dehydrogenase
11. A family of highly conserved glycosomal 2-hydroxyacid dehydrogenases from Phytomonas sp.
12. Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium
13. Conditional expression of glycosylphosphatidylinositol phospholipase C in Trypanosoma brucei
14. Rapid isolation of DNA from trypanosomatid protozoa using a simple 'mini-prep' procedure
15. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
16. Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing and increase in enzyme activity
17. Basic local alignment search tool
18. The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA"s
19. A rapid quantitative method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
20. The use of least squares in the data analysis