Detection and quantification of the red tide dinoflagellate Karenia brevis by real-time nucleic acid sequence-based amplification

Applied and Environmental Microbiology

Volumn 70, Issue 8, 2004, Pages 4727-4732

  Casper, Erica T ^a   Paul, John H ^a   Smith, Matthew C ^a   Gray, Michael ^a  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLIFICATION; ASSAYS; BACTERIA; CELLS; CLONING; CONCENTRATION (PROCESS); ISOTHERMS; MARINE ENGINEERING; RNA;

DIAGNOSTIC TESTING; ISOTHERMAL METHOD; NUCLEIC ACID SEQUENCE-BASED AMPLIFICATION (NASBA);

NUCLEIC ACIDS;

MICROBIOLOGY;

ANALYTIC METHOD; ARTICLE; CELL COUNT; DINOFLAGELLATE; GENE TARGETING; MARINE ENVIRONMENT; NONHUMAN; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; PROTOZOAL INFECTION; QUANTITATIVE ANALYSIS; RED TIDE; RNA TRANSCRIPTION; SAMPLING; TOXIN ANALYSIS; WILDLIFE CONSERVATION;

ANIMALS; DINOFLAGELLIDA; DNA, PROTOZOAN; ENVIRONMENTAL MONITORING; RIBULOSE-BISPHOSPHATE CARBOXYLASE; RNA, MESSENGER; SEAWATER; SELF-SUSTAINED SEQUENCE REPLICATION; SENSITIVITY AND SPECIFICITY;

DINOPHYCEAE; KARENIA; KARENIA BREVIS; PROTOZOA;

EID: 4143123572     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.8.4727-4732.2004     Document Type: Article

References (21)

1. Adachi, M., Y. Sako, and Y. Ishida. 1996. Identification of the toxic dinoflagellates Alexandrium catenella and A. tamarense (Dinophyceae) using DNA probes and whole-cell hybridization. J. Phycol. 32:1049-1052.
2. Culverhouse, P. F., R. Williams, B. Reguera, V. Henry, and S. Gonzalez-Gil. 2003. Do experts make mistakes? A comparison of human and machine identification of dinoflagellates. Mar. Ecol. Prog. Ser. 247:17-25.
3. Davey, C., and L. T. Malek. 1989. Nucleic acid amplification process. European patent EP 0329822.
4. Godhe, A., S. K. Otta, A.-S. Rehnstam-Holm, I. Karunasagar, and I. Kamnasagar. 2001. Polymerase chain reaction in detection of Gymnodinium mikimotoi and Alexandrium minutum in field samples from southwest India. Mar. Biotechnol. 3:152-162.
5. Gray, M., B. Wawrik, J. Paul, and E. Casper. 2003. Molecular detection of the red tide dinoflagellate Karenia brevis in the marine environment. Appl. Environ. Microbiol. 69:5726-5730.
6. Gunther, G., R. H. Williams, C. C. Davis, and F. G. W. Smith. 1948. Catastrophic mass mortality of marine animals and coincident phytoplankton bloom on the west coast of Florida, November 1946 to August 1947. Ecol. Monogr. 18:311-324.
7. Hoagland, P., D. M. Anderson, Y. Kaoru, and A. W. White. 2002. The economic effects of harmful algal blooms in the United States: estimates, assessment issues, and information needs. Estuaries 25:819-837.
8. Landsberg, J. H. 2002. The effects of harmful algal blooms on aquatic organisms. Rev. Fish. Sci. 10:113-390.
9. Landsberg, J. H., and K. A. Steidinger. 1998. A historical review of Gymnodinium breve red tides implicated in mass mortalities of the manatee (Trichechus mantus latirostris) in Florida, USA, p. 97-100. In B. Reguera, J. Blanco, M. L. Fernandez, and T. Wyatt (ed.), Proceedings of the 8th International Conference on Harmful Algae. Xunta de Galicia and Intergovernmental Oceanographie Commission of UNESCO, Vigo, Spain.
10. Litaker, R. W., M. W. Vandersea, S. R. Kibler, K. S. Reece, N. A. Stokes, K. A. Steidinger, D. F. Millie, B. J. Bendis, R. J. Pigg, and P. A. Tester. 2003. Identification of Pfiesteria piscicida (Dinophyceae) and Pfiesteria-like organisms using internal transcribed spacer-specific PCR assays. J. Phycol. 39:754-761.
11. Loret, P., T. Tengs, T. A. Villareal, H. Singler, B. Richardson, P. McGuire, S. Morton, M. Busman, and L. Campbell. 2002. No difference found in ribosomal DNA sequences from physiologically diverse clones of Kareniu brevis (Dinophyceae) from the Gulf of Mexico. J. Plankton Res. 24:735-739.
12. Millie, D. F., O. M. Schofield, G. J. Kirkpatrick, G. Johnsen, P. A. Tester, and B. T. Vinyard. 1997. Detection of harmful algal blooms using photopigments and absorption signatures: a case study of the Florida red tide dinoflagellate, Gymnodinium breve. Limnol. Oceanogr. 42:1240-1251.
13. Millie, D. F., G. J. Kirkpatrick, G. Johnsen, O. M. E. Achofield, and T. J. Evens. 2001. Using absorbance and fluorescence spectra to discriminate microalgal phylogenetic groups and taxa. J. Phycol. 37:35.
14. Oernolfsdottir, E. B., J. L. Pinckney, and P. A. Tester. 2003. Quantification of the relative abundance of the toxic dinoflagellate, Karenia brevis (Dinophyta) using unique photopigments. J. Phycol. 39:449-457.
15. Peperzak, L., B. Sandee, C. Scholin, P. Miller, and L. van Nieuwerburgh. 2000. Application and flow cytometric detection of antibody and rRNA probes to Gymnodinium mikimotoi (Dinophyceae) and Pseudo-nitzschia multiseries (Bacillariophyceae), p. 206-209. In G. M. Hallegraff, S. I. Blackburn, C. J. Bolch, and R. J. Lewis (ed.), Harmful algal blooms 2000. IOC-UNESCO, Paris, France.
16. Pierce, R. H., and G. J. Kirkpatrick. 2001. Innovative techniques for harmful algal toxin analysis. Environ. Toxicol. Chem. 20:107-114.
17. Stumpf, R. P. 2001. Applications of satellite ocean color sensors for monitoring and predicting harmful algal blooms. Hum. Ecol. Risk Assess. 7:1363-1368.
18. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
19. Tyagi, S., and F. R. Kramer. 1996. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14:303-308.
20. Van Dolah, F. M., D. Roelke, and R. M. Greene. 2001. Health and ecological impacts of harmful algal blooms: risk assessment needs. Hum. Ecol. Risk Assess. 7:1329-1345.
21. Weusten, J. J. A. M., W. M. Carpay, T. A. M. Oosterlaken, M. C. A. van Zuijlen, and P. A. van de Wiel. 2002. Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogenous detection using molecular beacons. Nucleic Acids Res. 30:e26. [Online.]