DNA polymerase X from Deinococcus radiodurans possesses a structure-modulated 3′→ 5′ exonuclease activity involved in radioresistance

Molecular Microbiology

Volumn 60, Issue 1, 2006, Pages 165-176

  Blasius, Melanie ^a   Shevelev, Igor ^a   Jolivet, Edmond ^b   Sommer, Suzanne ^b   Hübscher, Ulrich ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; DNA POLYMERASE X; EXONUCLEASE; GLYCINE; MUTANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELL SURVIVAL; DEINOCOCCUS RADIODURANS; DNA REPAIR; DNA STRAND BREAKAGE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; GAMMA IRRADIATION; IN VIVO STUDY; KNOCKOUT GENE; NONHUMAN; PHP DOMAIN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FAMILY; RADIOSENSITIVITY; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS); DEINOCOCCUS RADIODURANS;

EID: 33644841882     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2006.05077.x     Document Type: Article

References (28)

1. Aravind L. Koonin EV. 1998 Phosphoesterase domains associated with DNA polymerases of diverse origins Nucleic Acids Res 26 3746 3752
2. Battista JR. 1997 Against all odds: the survival strategies of Deinococcus radiodurans Annu Rev Microbiol 51 203 224
3. Battista JR. Earl AM. Park MJ. 1999 Why is Deinococcus radiodurans so resistant to ionizing radiation? Trends Microbiol 7 362 365
4. Bebenek K. Garcia-Diaz M. Blanco L. Kunkel TA. 2003 The frameshift infidelity of human polymerase lambda. Implications for function J Biol Chem 278 34685 34690
5. Bonacossa de Almeida C. Coste G. Sommer S. Bailone A. 2002 Quantification of RecA protein in Deinococcus radiodurans reveals involvement of RecA, but not LexA, in its regulation Mol Genet Genomics: MGG 268 28 41
6. Cox MM. Battista JR. 2005 Deinococcus radiodurans-the consummate survivor Nat Rev Microbiol 3 882 892
7. Daly MJ. Gaidamakova EK. Matrosova VY. Vasilenko A. Zhai M. Venkateswaran A. et al. 2004 Accumulation of Mn(II) in Deinococcus radiodurans facilitates gamma-radiation resistance Science 306 1025 1028
8. D'Amours D. Jackson SP. 2002 The Mre11 complex: at the crossroads of dna repair and checkpoint signalling Nat Rev Mol Cell Biol 3 317 327
9. Friedrich-Heineken E. Henneke G. Ferrari E. Hübscher U. 2003 The acetylatable lysines of human Fen1 are important for endo- and exonuclease activities J Mol Biol 328 73 84
10. Harris DR. Tanaka M. Saveliev SV. Jolivet E. Earl AM. Cox MM. Battista JR. 2004 Preserving genome integrity: the DdrA protein of Deinococcus radiodurans R1 PLoS Biol 2 e304
11. Kikuchi MN. Narumi I. Kitayama S. Watanabe H. Yamamoto K. 1999 Genomic organization of the radioresistant bacterium Deinococcus radiodurans: physical map and evidence for multiple replicons FEMS Microbiol Lett 174 151 157
12. Kunkel TA. 1985 The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations J Biol Chem 260 5787 5796
13. Lecointe F. Shevelev IV. Bailone A. Sommer S. Hübscher U. 2004 Involvement of a DNA polymerase X from the radioresistant organism Deinococcus radiodurans in double strand break repair Mol Microbiol 53 1721 1730
14. Lutz R. Bujard H. 1997 Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements Nucleic Acids Res 25 1203 1210
15. Ma JL. Kim EM. Haber JE. Lee SE. 2003 Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences Mol Cell Biol 23 8820 8828
16. Maga G. Shevelev I. Ramadan K. Spadari S. Hubscher U. 2002 DNA polymerase theta purified from human cells is a high-fidelity enzyme J Mol Biol 319 359 369
17. Makarova KS. Aravind L. Wolf YI. Tatusov RL. Minton KW. Koonin EV. Daly MJ. 2001 Genome of the extremely radiation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics Microbiol Mol Biol Rev 65 44 79
18. Mazur DJ. Perrino FW. 2001 Excision of 3′ termini by the TREX1 and TREX2 3′→5′ exonucleases. Characterization of the recombinant proteins J Biol Chem 276 17022 17029
19. Moore JK. Haber JE. 1996 Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae Mol Cell Biol 16 2164 2173
20. Oliveros M. Yáñez RJ. Salas ML. Salas J. Viñuela E. Blanco L. 1997 Characterization of an African Swine Fever Virus 20-kDa DNA polymerase involved in DNA repair J Biol Chem 272 30899 30910
21. Ramadan K. Shevelev I. Hubscher U. 2004 The DNA-polymerase-X family: controllers of DNA quality? Nat Rev Mol Cell Biol 5 1038 1043
22. Shevelev IV. Hubscher U. 2002 The 3′→5′ exonucleases Nat Rev Mol Cell Biol 3 364 376
23. Trujillo KM. Sung P. 2001 DNA structure-specific nuclease activities in the Saccharomyces cerevisiae Rad50*Mre11 complex J Biol Chem 276 35458 35464
24. Trujillo KM. Yuan SS. Lee EY. Sung P. 1998 Nuclease activities in a complex of human recombination and DNA repair factors Rad 50, Mre11, and p95 J Biol Chem 273 21447 21450
25. Wang TSF. Conger KL. Copeland WC. Arroyo MP. 1999 Eukaryotic DNA polymerases In Eukaryotic DNA Replication: A Practical Approach Cotterill S. (ed.) Oxford Oxford University Press pp. 67 92
26. Weller GR. Kysela B. Roy R. Tonkin LM. Scanlan E. Della M. et al. 2002 Identification of a DNA nonhomologous end-joining complex in bacteria Science 297 1686 1689
27. Wilson TE. Lieber MR. 1999 Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase beta (Pol4)-dependent pathway J Biol Chem 274 23599 23609
28. Zuker M. 2003 Mfold web server for nucleic acid folding and hybridization prediction Nucleic Acids Res 31 3406 3415