Mechanism of action and determination of the best substrate for a thrombin-like enzyme from Lachesis muta muta venom by regression analysis of the kinetic parameters determined with peptidyl p-nitroanilide substrates

Toxicon

Volumn 47, Issue 4, 2006, Pages 453-458

  Magalhães, Henrique P B ^a   Magalhães, Arinos ^b   Juliano, Luiz ^c   Nelson, David Lee ^a   Rogana, Edyr ^a  

^a FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

^b FUNDAÇÃO EZEQUIEL DIAS   (Brazil)

^c FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Lachesis muta muta; Thrombin like enzyme; Tripeptidyl p nitroanilides

Indexed keywords

4 NITROANILINE; LEUCINE; PEPTIDE 4 NITROANILIDE; PEPTIDE DERIVATIVE; SNAKE VENOM; THROMBIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME SPECIFICITY; PRIORITY JOURNAL; REGRESSION ANALYSIS; SNAKE;

ANILIDES; CROTALID VENOMS; KINETICS; REGRESSION ANALYSIS; SUBSTRATE SPECIFICITY;

LACHESIS MUTA MUTA;

EID: 33644837215     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2006.01.001     Document Type: Article

References (24)

1. G. Amiconi, A. Amoresano, G. Bourmis, A. Brancaccio, R. De Cristofaro, and A. De Pascalis A novel venombin B from Agkistrodon contortrix contortrix: evidence for recognition properties in the surface around the primary specificity pocket different from thrombin Biochemistry 39 2000 10294 10308
2. W. Bode, D. Turk, and A. Karshikov The refined 1.9-Angstrom X-ray crystal structure of D-Phe-Pro-Arg-chloromethylketone-inhibited human [alpha]-thrombin: structure analysis, overall structure, electrostatic properties, detailed active site geometry and structure-function relationships Prot. Sci. 1 1992 426 471
3. H.C. Castro, D.M. Silva, C. Craik, and R.B. Zingali Structural features of a snake thrombin-like enzyme: thrombin and trypsin on a single catalytic platform Biochim. Biophys. Acta 1547 2001 183 195
4. H.C. Castro, R.B. Zingali, M.G. Albuquerque, M. Pujol-Luz, and C.R. Rodrigues Snake thrombin-like enzymes: from reptilase to now Cell. Mol. Life Sci. 61 2004 843 856
5. E. Di Cera, Q.D. Dang, and Y.M. Ayala Molecular mechanism of thrombin function Cell. Mol. Life Sci. 53 1997 701 730
6. L. Earps, and P.M. Shoolingin-Jordan Molecular modelling of batroxobin on Kallikreins Biochem. Soc. Trans. 26 1998 283
7. S.M. Free, and J.W. Wilson A mathematical contribution to structure-activity studies J. Med. Chem. 7 1964 395 399
8. Y.W. Guo, T.Y. Chang, K.T. Lin, H.W. Liu, K.C. Shih, and S.H. Cheng Cloning and functional expression of the mucrosobin protein, a beta-fibrinogenase of Trimeresurus mucrosquamatus (Taiwan Habu) Protein Expt. Purif. 23 2001 483 490
9. A.H. Henschen-Edman, I. Theodor, B.F. Edwards, and H. Pirkle Crotalase, a fibrinogen-clotting snake venom thrombin-like enzyme: primary structure and evidence for a fibrinogen recognition exosite different from thrombin Thromb. Haemostasis 81 1999 81 86
10. R.G. Junqueira, and M.L. Mares-Guia A statistical treatment for solution of a family of simultaneous equations derived from enzyme inhibition studies Brazilian. J. Med. Biol. Res. 23 1990 773 784
11. A. Magalhães, B.C.B. Da Fonseca, C.R. Diniz, J. Gilroy, and M. Richardson The complete amino acid sequence of a thrombin-like enzyme/gyroxin analogue from venom of bushmaster snake (Lachesis muta muta) FEBS Lett. 329 1993 116 120
12. A. Magalhães, M.R. Monteiro, H.P.B. Magalhães, M. Mares-Guia, and E. Rogana Thrombin-like enzyme from Lachesis muta muta venom: isolation and topographical analysis of its active site structure by means of the binding of amidines and guanidines as competitive inhibitors Toxicon 35 10 1997 1549 1559
13. R.C. Maroun Molecular basis for the partition of the essential functions of thrombin among snake venom serine proteinases Haemostasis 31 2001 247 256
14. N. Marsh, and V. Williams Practical applications of snake venom toxins in haemostasis Toxicon 45 8 2005 1171 1181
15. M.A. Parry, U. Jacob, R. Huber, A. Wisner, C. Bon, and W. Bode The crystal structure of the novel snake venom plasminogen activator TSV-PA: a prototype structure for snake venom serine proteinase Structure 6 1998 1195 1206
16. H. Pirkle Thrombin-like enzymes from snake venoms: an update inventory Thromb. Haemostasis 79 1998 675 683
17. B. Polack Thrombin a multifunctional enzyme Ann. Biol. Clin. (Paris) 61 2003 23 31
18. M. Pozsgay, R. Gáspar, S. Bajusz, and P. Elódi A method for designing peptide substrates for proteases Eur. J. Biochem. 95 1979 115 119
19. I. Schecther, and A. Berger On the size of active site in proteases. I-Papain Biochem. Biophys. Res. Commun. 27 1967 157 162
20. A.M.V. Silveira, A. Magalhães, C.R. Diniz, and E.B. Oliveira Purification and properties of the thrombin-like enzyme from the venom of Lachesis muta muta Int. J. Biochem. 21 1989 863 872
21. R. Tatematsu, Y. Komori, and T. Nikai A new thrombin-like enzyme, flavoridiobin, from the venom of Trimeresurus flavoridis (habu) J. Nat. Toxins 9 2001 327 339
22. Y.M. Wang, S.R. Wang, and I.H. Tsai Serine protease isoforms of Deinagkistrodon acutus venom: cloning, sequencing and phylogenetic analysis Biochem. J. 354 2001 161 168
23. K. Weber, and M. Osborn Proteins and sodium dodecyl sulfate: Molecular weight determination on polyacrylamide gels and related procedures H. Neurath R.L. Hill The Proteins vol. 1 1976 Academic Press New York 179 223 (Chapter 3)
24. G.N. Wilkinson Statistical estimations in enzyme kinetics Biochem. J. 80 1961 324 332