Use of fluorescence to monitor the incorporation of horseradish peroxidase into a sol-gel derived medium

Biophysical Chemistry

Volumn 120, Issue 2, 2006, Pages 81-86

  Hungerford, Graham ^a   Rei, Ana ^a   Ferreira, M Isabel C ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Catalytic activity; Energy transfer; Molecular dynamics; Nile red

Indexed keywords

FLUORESCENT DYE; HORSERADISH PEROXIDASE;

AGING; ARTICLE; CATALYSIS; ENERGY TRANSFER; ENZYME ACTIVITY; FLUORESCENCE; GEL; MOLECULAR DYNAMICS; PRIORITY JOURNAL; SPECTROFLUOROMETRY; TEMPERATURE;

FLUORESCENT DYES; HORSERADISH PEROXIDASE; OXAZINES; PHASE TRANSITION; SPECTROMETRY, FLUORESCENCE; TEMPERATURE;

ARMORACIA RUSTICANA;

EID: 33644760262     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bpc.2005.10.009     Document Type: Article

References (24)

1. C.J. Brinker, and G.W. Scherer Sol-Gel Science: The Physics and Chemistry of Sol-Gel Processing 1990 Academic Press
2. L.L. Hench, and J.K. West The sol-gel process Chem. Rev. 90 1990 33 72
3. W. Jin, and J.D. Brennan Properties and applications of proteins encapsulated within sol-gel derived materials Anal. Chim. Acta 461 2002 1 36
4. R. Reisfeld Spectroscopy and applications of molecules in glasses J. Non-Cryst. Solids 121 1990 254 266
5. A.C. Pierre The sol-gel encapsulation of enzymes Biocatal. Biotransform. 22 2004 145 170
6. Y.-W. Cho, and S. Han Catalytic activities of glass-encapsulated horseradish peroxidase at extreme pHs and temperatures Bull. Korean Chem. Soc. 20 1999 1363 1364
7. F.A. ElEssi, A.Z.A. Zuhri, S.I. Al-Khalil, and M.S. Abdel-Latif Spectrophotometric determination of enzymatically generated hydrogen peroxide using sol-gel immobilized horseradish peroxidase Talanta 44 1997 2051 2058
8. E.H. Lan, B.C. Dave, J.M. Fukuto, B. Dunn, J.I. Zink, and J.S. Valentine Synthesis of sol-gel encapsulated heme proteins with chemical sensing properties J. Mater. Chem. 9 1999 45 53
9. E.N. Kadnikova, and N.M. Kostić Sol-gel glass is not necessarily an inert matrix for enzyme encapsulation. Catalysis of sulfoxidation by silica J. Non-Cryst. Solids 282 2001 63 68
10. R.B. Bhatia, C.J. Brinker, A.K. Gupta, and A.K. Singh Aqueous sol-gel process for protein encapsulation Chem. Mater. 12 2000 2434 2441
11. D.M. Liu, and I.W. Chen Encapsulation of protein molecules in transparent porous silica matrices via an aqueous colloidal sol-gel process Acta Mater. 47 1999 4535 4544
12. B. Dunn, J.M. Miller, B.C. Dave, J.S. Valentine, and J.I. Zink Strategies for encapsulating biomolecules in sol-gel matrices Acta Mater. 46 1998 737 741
13. Y. Liu, M. Wang, J. Li, Z. Li, P. He, H. Liu, and J. Li Highly active horseradish peroxidase immobilized in 1-butyl-3-methylimidazolium tetrafluoroborate room-temperature ionic liquid based sol-gel host materials Chem. Commun. 2005 1778 1780
14. N. Ghoneim Photophysics of nile red in solution steady state spectroscopy Spectrochim. Acta, Part A 56 2000 1003 1010
15. A.K. Dutta, K. Kamada, and K. Ohta Spectroscopic studies of nile red in organic solvents and polymers J. Photochem. Photobiol., A Chem. 93 1996 57 64
16. G. Hungerford, A. Rei, M.I.C. Ferreira. Studies on the interaction of nile red with horseradish peroxidase in solution. FEBS J. (In press).
17. R.E. Childs, and W.G. Bardsley The steady-state kinetics of peroxidase with 2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen Biochem. J. 145 1975 93 103
18. E.N. Kadnikova, and N.M. Kostić Oxidation of ABTS by hydrogen peroxide catalysed by horseradish peroxidase encapsulated into sol-gel glass. Effects of glass matrix on reactivity J. Mol. Catal., B Enzym. 18 2002 39 48
19. T.M.R. Viseu, G. Hungerford, A.F. Coelho, and M.I.C. Ferreira Dye-host interactions for local effects recognition in homogeneous and nanostructured media J. Phys. Chem., B 107 2003 13300 13312
20. V. Sanz, S. deMarcos, J.R. Castillo, and J. Galbán Application of molecular absorption properies of horseradish peroxidase for self-indicating enzymatic interactions and analytical methods J. Am. Chem. Soc. 127 2005 1038 1048
21. D.K. Eggers, and J.S. Valentine Molecular confinement influences protein structure and enhances thermal protein stability Protein Sci. 10 2001 250 261
22. J.D. Badjić, and N.M. Kostić Effects of encapsulation in sol-gel silica glass on esterase activity, conformational stability, and unfolding of bovine carbonic anhydrase II Chem. Mater. 11 1999 3671 3679
23. K. Chattopadhyay, and S. Mazumdar Structural and conformational stability of horseradish peroxidase: effect of temperature and pH Biochemistry 39 2000 263 270
24. D.G. Pina, A.V. Shnyrova, F. Gavilanes, A. Rodríguez, F. Leal, M.G. Roig, I.Y. Sakharov, G.G. Zhadan, E. Villar, and V.L. Shnyrov Thermally induced conformational changes in horseradish peroxidase Eur. J. Biochem. 268 2001 120 126