Architecture of a fungal fatty acid synthase at 5 Å resolution

Science

Volumn 311, Issue 5765, 2006, Pages 1263-1267

  Jenni, Simon ^a   Leibundgut, Marc ^a   Maier, Timm ^a   Ban, Nenad ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER CONCENTRATION; CRYSTALLOGRAPHY; ENZYMES; FUNGI; PROTEINS; SUBSTRATES; SYNTHESIS (CHEMICAL);

ACTIVE SITES; FUNGAL FATTY ACID; MATRIX; MULTIENZYME; REACTION CHAMBERS; REACTION CYCLE; STRUCTURAL FEATURES;

FATTY ACIDS;

ACYL CARRIER PROTEIN; FATTY ACID SYNTHASE;

ENZYME ACTIVITY; FATTY ACID; FUNGUS;

ARTICLE; CATALYSIS; ELECTRON; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; FATTY ACID SYNTHESIS; FUNGUS; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

ACYL CARRIER PROTEIN; ASCOMYCOTA; BINDING SITES; CATALYTIC DOMAIN; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; FATTY ACID SYNTHETASE COMPLEX; FATTY ACIDS; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID;

FUNGI;

EID: 33644663441     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1123251     Document Type: Article

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39. All data were collected at the Swiss Light Source (SLS, Paul Scherrer Institute, Villigen, Switzerland). We thank C. Schutze-Briese, A. Wagner, E. Pohl, T. Tomizaki, and S. Gutmann for their outstanding support at the SLS; our colleagues M. Steiner, B. Mikolasek, and C. Frick for their help in FAS preparation; members of the Ban laboratory for suggestions and discussions; H. Gross and W. Baumeister for their help in interpreting electron microscopic images; G. Kaim, T. Meier, U. Bauer, and A. Lehmann for assisting in cell fermentation; C. Vonrhein for a prerelease version of the program SHARP; and D. Sargent for technical assistance. T. lonuginosus expressed sequence tag sequence data were produced by the Fungal Genomics Project (fungalgenomics.concordia.ca) . We thank A. Tsang for providing the raw sequencing data and P. Pencek for providing the cryoelectron microscopic map of the Saccharomyces cerevisiae FAS. S.J. was supported by the Swiss Study Foundation and thanks the instructors of the 2003 x-ray course at Cold Spring Harbor Laboratory. This work was supported by the Swiss National Science Foundation (SNSF) and the National Center of Competence in Research Structural Biology program of the SNSF. Coordinates of the rigid-body fitted homologous domains have been deposited in the Protein Data Bank with accession code 2CDH.