Efficient cleavage by signal peptide peptidase requires residues within the signal peptide between the core and E1 proteins of hepatitis C virus strain J1

Journal of General Virology

Volumn 87, Issue 3, 2006, Pages 623-627

  Graham Hope, R ^a   McElwee, Marion J ^a   McLauchlan, John ^a  

^a INSTITUTE OF VIROLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CORE PROTEIN; DNA FRAGMENT; GLYCOPROTEIN E1; SIGNAL PEPTIDASE; SIGNAL PEPTIDE; VIRUS DNA;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL STRAIN BHK; CELLULAR DISTRIBUTION; EXPRESSION VECTOR; GENE CONSTRUCT; GENE MUTATION; HEPATITIS C VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; RESIDUE ANALYSIS; SEQUENCE ANALYSIS; STRAIN DIFFERENCE; VIRUS MUTANT; VIRUS STRAIN; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ENDOPEPTIDASES; CELL LINE; HEPACIVIRUS; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; VIRAL CORE PROTEINS; VIRAL ENVELOPE PROTEINS;

ANIMALIA; DNA VIRUSES; HEPATITIS C VIRUS;

EID: 33644585599     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/vir.0.81371-0     Document Type: Article

References (13)

1. Clayton, R. F., Owsianka, A., Aitken, J., Graham, S., Bhella, D. & Patel, A. H. (2002). Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C virus-like particles. J Virol 76, 7672-7682.
2. Hope, R. G. & McLauchlan, J. (2000). Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. J Gen Virol 81, 1913-1925.
3. Hope, R. G., Murphy, D. J. & McLauchlan, J. (2002). The domains required to direct core proteins of hepatitis C virus and GB virus-B to lipid droplets share common features with plant oleosin proteins. J Biol Chem 277, 4261-4270.
4. Hüssy, P., Langen, H., Mous, J. & Jacobsen, H. (1996). Hepatitis C virus core protein: carboxy-terminal boundaries of two processed species suggest cleavage by a signal peptide peptidase. Virology 224, 93-104.
5. Kawooya, J. K., Emmons, T. L., Gonzalez-DeWhitt, P. A., Camp, M. C. & D'Andrea, S. C. (2003). Electrophoretic mobility of Alzheimer's amyloid-β peptides in urea-sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem 323, 103-113.
6. Klafki, H.-W., Wiltfang, J. & Staufenbiel, M. (1996). Electrophoretic separation of βA4 peptides (1-40) and (1-42). Anal Biochem 237, 24-29.
7. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
8. Lemberg, M. K. & Martoglio, B. (2002). Requirements for signal peptide peptidase-catalyzed intramembrane proteolysis. Mol Cell 10, 735-744.
9. Lemberg, M. K. & Martoglio, B. (2003). Analysis of polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis alongside in vitro-generated reference peptides. Anal Biochem 319, 327-331.
10. McLauchlan, J., Lemberg, M. K., Hope, G. & Martoglio, B. (2002). Intramembrane proteolysis promotes trafficking of hepatitis C virus core protein to lipid droplets. EMBO J 21, 3980-3988.
11. Okamoto, K., Moriishi, K., Miyamura, T. & Matsuura, Y. (2004). Intramembrane proteolysis and endoplasmic reticulum retention of hepatitis C virus core protein. J Virol 78, 6370-6380.
12. Weihofen, A., Lemberg, M. K., Ploegh, H. L., Bogyo, M. & Martoglio, B. (2000). Release of signal peptide fragments into the cytosol requires cleavage in the transmembrane region by a protease activity that is specifically blocked by a novel cysteine protease inhibitor. J Biol Chem 275, 30951-30956.
13. Weihofen, A., Lemberg, M. K., Friedmann, E., Rueeger, H., Schmitz, A., Paganetti, P., Rovelli, G. & Martoglio, B. (2003). Targeting presenilin-type aspartic protease signal peptide peptidase with γ-secretase inhibitors. J Biol Chem 278, 16528-16533.