Biochemical and genetic analyses of a novel γ-cyclodextrin glucanotransferase from an alkalophilic Bacillus clarkii 7364

Journal of Biochemistry

Volumn 133, Issue 3, 2003, Pages 317-324

  Takada, Masayasu ^a   Nakagawa, Yoshinori ^a   Yamamoto, Mikio ^a  

^a Fuji   (Japan)

Author keywords

CGTase; Amino acid sequence; Bacillus clarkii; Cyclic specificity; Cyclodextrin

Indexed keywords

GAMMA CYCLODEXTRIN; GLUCANOTRANSFERASE; PRIMER DNA; TRANSFERASE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS; BACILLUS CLARKII; BACTERIUM IDENTIFICATION; CHEMICAL ANALYSIS; CYCLIZATION; ENZYME SPECIFICITY; GEL FILTRATION; GENE AMPLIFICATION; GENETIC ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

AMINO ACID SEQUENCE; BACILLUS; CYCLODEXTRINS; GAMMA-CYCLODEXTRINS; GLUCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PROTEIN BINDING;

BACILLUS CLARKII; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; POSIBACTERIA; SOLANUM TUBEROSUM;

EID: 0037368504     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvg043     Document Type: Article

References (29)

1. Duchêne, D. (1991) Cyclodextrins and Their Industrial Uses, editions de Santé, Paris
2. van der Veen, B.A., Uitdehaag, J.C.M., Dijkstra, B.W., and Dijkhuizen, L. (2000) Engineering of cyclodextrin glycosyltransferase reaction and product specificity. Bicochim. Biophys. Acta 1543, 336-360
3. Janecek, S., (1997) α-Amylase family: molecular biology and evolution. Prog. Biophys. Mol. Biol. 67, 67-97
4. Uitdehaag, J.C.M., van Alebeek, G.W.M., van der Veen, B.A., Dijkhuizen, L., and Dijkstra, B.W. (2000) Structures of maltohexaose and maltoheptaose bound at the donor sites of cyclodextrin glycosyltransferase give insight into the mechanisms of transglycosylation activity and cyclodextrin size specificity. Biochemistry 39, 7772-7780
5. Ebglbrecht, A., Harrer, G., Lebert, M., and Schmid, G. (1990) Biochemical and genetic characterization of a CGTase from an alkalophilic bacterium forming primary γ-cyclodextrin in Proceeding of the 5th International Symposium on Cyclodextrins (Hubber, O. and Szejyli, J., eds.) pp. 25-31, Kluwer Academic
6. Martins, R.F. and Hatii-Kaul, R. (2002) A new cyclodextrin glycosyltransferase from an alkaliphilic Bacillus agaradhaerens isolate: purification and characterization. Enzyme Microb. Technol. 30, 116-124
7. Takano, T., Fukuda, M., Monma, M., Kobayashi, S., Kainuma, K., and Yamane, K. (1986) Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene. J. Bacteriol. 166, 1118-1122
8. Penninga, D., Strokopytov, B., Rozeboom, H.J., Lawson, C.L., Dijkstra, B.W., Bergsma, J., and Dijkhuizen, L. (1995) Site-directed mutations in tyrosine 195 of cyclodextrin glycosyl-transferase from Bacillus circulans strain 251 affect activity and product specificity. Biochemistry 34, 3368-3376
9. Kato, T. and Horikoshi, K. (1986) A new γ-cyclodextrin forming enzyme produced by Bacillus subtilis no. 313. J. Jpn. Soc. Starch Sci. 34, 137-143
10. Nakamura, A., Haga, K., and Yamane, K. (1994) Four aromatic residues in the active center of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011: effects of replacements on substrate binding and cyclization characteristics. Biochemistry 33, 929-9936
11. Sin, K.A., Nakamura, A., Masaki, H., and Uozumi, T. (1993) Extracellular production of Bacillus ohbensis cyclodextrin glucanotransferase by B. subtilis. Biosci. Biotechnol. Biochem. 57, 346-347
12. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265-275
13. Fuwa, H. (1954) A new method for microdetermination of amylase activity by the use of amylose as the substrate. J. Biochem. 41, 583-603
14. Kato, T. and Horikoshi, K. (1984) Colorimetric determination of γ-cyclodextrin. Anal. Chem. 56, 1738-1740
15. Declerk, N., Joyet, P., Coq, D.L., and Heslot, H. (1998) Integration, amplification and expression of the Bacillus licheniformis α-amylase gene in Bacillus subtilis chromosome. J. Biotechnol. 8, 23-38
16. Smith, L.M., Sanders, J.Z., Kaiser, R.J., Hughes, P., Dodd, C., Connell, C.R., Heiner, C., Kent, S.B.H., and Hood, L.E. (1986) Fluorescence detection in automated DNA sequence analysis. Nature 321, 674-679
17. Collins, F.S. and Weissman, S.M. (1984) Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method. Proc. Natl Acad. Sci. USA 81, 6812-6816
18. Nielsen, P., Fritze, D., and Priest, F.G. (1995) Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745-1761
19. Yumoto, I., Yamazaki, K., Sawabe, T., Nakano, K., Kawasaki, K., Ezura, Y., and Shinano H. (1998) Bacillus horti sp. nov. a new gram-negative alkaliphilic bacillus. Int. J. Syst. Bacteriol. 23, 238-242
20. Hagihara, H., Igarashi, K., Hayashi, Y., Endo, K., Kitayamama, K., Ozaki, K., Kawai S., and Ito S. (2001) Novel α-amylase that is highly resistant to chelating reagents and chemical oxidants from the alkaliphilic Bacillus isolate KSM-K-38. Appl. Environ. Microbiol. 67, 1744-1750
21. Murray, C.L. and Rabinowitz, J.C. (1982) Nucleotide sequences of transcription and translation regions in Bacillus phage Φ29 early genes. J. Biol. Chem. 257, 1053-1062
22. Uitdehaag, J.C.M., Kalk, K.H., van der Veen, B.A., Dijkhuizen, L., and Dijkstra, B.W. (1999) The cyclization mechanism of cyclodextrin glycosyltransferase (CGTase) as revealed by a γ-cyclodextrin-CGTase complex at 1.8-Å resolution. J. Biol. Chem. 274, 34868-34876
23. Strokopytov, B., Knegtel, R.M.A., Penninga, D., Rozeboom, H.J., Kalk, K.H., Dijkhuizen, L., and Dijkstra, B.W. (1996) Structure of cyclodextrin glycosyl-transferase complexed with a maltononaose inhibitor at 2.6 Å resolution. Implication for product specificity. Biochemistry 35, 4241-4249
24. Sin, K.A., Nakamura, A., Masaki, H., Matsuura, Y., and Uozumi, T. (1994) Replacement of an amino acid residue of cyclodextrin glucanotransferase of Bacillus ohbensis doubles the production of γ-CD. J. Biotechnol. 32, 283-288
25. Parsiegla, G., Schmidt, A.K., and Schulz, G.E. (1998) Substrate binding to a cyclodextrin glycosyltransferase and mutations increasing the γ-cyclodextrin production. Eur. J. Biochem. 255, 710-717
26. Fujiwara, S., Kakihara, H., Sakaguchi, T, and Imanaka, T. (1992) Analysis of mutations in cyclodextrin glucanotransferase from Bacillus stearothermophilus which affect cyclization characteristics and thermostability. J. Bacteriol. 174, 7478-7481
27. Fujita, Y., Tsybouchi, H., Inagi, Y., Tomita, K., Ozaki, A., and Nakanishi, K. (1990) Purification and properties of cyclodextrin glycosyltransferase from Bacillus sp. AL-6. J. Ferment. Bioeng. 70, 150-154
28. Mori, S., Hirose, S., Oya, T., and Kitahata, S. (1994) Purification and properties of cyclodextrin glucanotransferase from Brevibacterium sp. no. 9605. Biosci. Biotechnol. Biochem. 58, 1968-1972
29. Kitahata, S., Tsuyama, N., and Okada, S. (1974) Purification and some properties of cyclodextrin glycosyltransferase from a strain of Bacillus species. Agric. Biol. Chem. 38, 387-393