pWormgatePro enables promoter-driven knockdown by hairpin RNA interference of muscle and neuronal gene products in Caenorhabditis elegans

Invertebrate Neuroscience

Volumn 6, Issue 1, 2006, Pages 5-12

  Briese, Michael ^a   Esmaeili, Behrooz ^a,b   Johnson, Nicholas M ^c   Sattelle, David B ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

C. elegans; ORFeome; pWormgate; RNAi; sid 1; unc 22; unc 5

Indexed keywords

GENE PRODUCT;

ARTICLE; CAENORHABDITIS ELEGANS; CELL SPREADING; GENE SILENCING; GENE TECHNOLOGY; MOLECULAR CLONING; MUSCLE; NERVE; NEUROSYPHILIS; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

ANIMALS; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; CLONING, MOLECULAR; GENE SILENCING; MUSCLES; NEURONS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); RNA INTERFERENCE;

EID: 33644555529     PISSN: 13542516     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10158-005-0011-x     Document Type: Article

References (40)

1. Altun-Gultekin Z, Andachi Y, Tsalik EL, Pilgrim D, Kohara Y, Hobert O (2001) A regulatory cascade of three homeobox genes, ceh-10, ttx-3 and ceh-23, controls cell fate specification of a defined interneuron class in C. elegans. Development 128:1951-1969
2. Berezikov E, Bargmann CI, Plasterk RH (2004) Homologous gene targeting in Caenorhabditis elegans by biolistic transformation. Nucleic Acids Res 32:e40
3. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77:71-94
4. Colavita A, Culotti JG (1998) Suppressors of ectopic UNC-5 growth cone steering identify eight genes involved in axon guidance in Caenorhabditis elegans. Dev Biol 194:72-85
5. Colavita A, Krishna S, Zheng H, Padgett RW, Culotti JG (1998) Pioneer axon guidance by UNC-129, a C. elegans TGF-beta. Science 281:706-709
6. Culetto E, Sattelle DB (2000) A role for Caenorhabditis elegans in understanding the function and interactions of human disease genes. Hum Mol Genet 9:869-877
7. Fire A, Harrison SW, Dixon D (1990) A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans. Gene 93:189-198
8. Fire A, Albertson D, Harrison SW, Moerman DG (1991) Production of antisense RNA leads to effective and specific inhibition of gene expression in C. elegans muscle. Development 113:503-514
9. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811
10. Fraser A (2004) Towards full employment: using RNAi to find roles for the redundant. Oncogene 23:8346-8352
11. Gonczy P, Echeverri C, Oegema K, Coulson A, Jones SJ, Copley RR, Duperon J, Oegema J, Brehm M, Cassin E, Hannak E, Kirkham M, Pichler S, Flohrs K, Goessen A, Leidel S, Alleaume AM, Martin C, Ozlu N, Bork P, Hyman AA (2000) Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature 408:331-336
12. Hartley JL, Temple GF, Brasch MA (2000) DNA cloning using in vitro site-specific recombination. Genome Res 10:1788-1795
13. Hedgecock EM, Culotti JG, Hall DH (1990) The unc-5, unc-6, and unc-40 genes guide circumferential migrations of pioneer axons and mesodermal cells on the epidermis in C. elegans. Neuron 4:61-85
14. Johnson NM, Behm CA, Trowell SC (2005) Heritable and inducible gene knockdown in C. elegans using Wormgate and the ORFeome. Gene 359:26-34
15. Jones D, Russnak RH, Kay RJ, Candido EP (1986) Structure, expression, and evolution of a heat shock gene locus in Caenorhabditis elegans that is flanked by repetitive elements. J Biol Chem 261:12006-12015
16. Kamath RS, Ahringer J (2003) Genome-wide RNAi screening in Caenorhabditis elegans. Methods 30:313-321
17. Kamath RS, Fraser AG, Dong Y, Poulin G, Durbin R, Gotta M, Kanapin A, Le Bot N, Moreno S, Sohrmann M, Welchman DP, Zipperlen P, Ahringer J (2003) Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421:231-237
18. Kennedy S, Wang D, Ruvkun G (2004) A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans. Nature 427:645-649
19. Lamesch P, Milstein S, Hao T, Rosenberg J, Li N, Sequerra R, Bosak S, Doucette-Stamm L, Vandenhaute J, Hill DE, Vidal M (2004) C. elegans ORFeome version 3.1: increasing the coverage of ORFeome resources with improved gene predictions. Genome Res 14:2064-2069
20. Lee YS, Carthew RW (2003) Making a better RNAi vector for Drosophila: use of intron spacers. Methods 30:322-329
21. Maduro M, Pilgrim D (1995) Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system. Genetics 141:977-988
22. Maeda I, Kohara Y, Yamamoto M, Sugimoto A (2001) Largescale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi. Curr Biol 11:171-176
23. Mello CC, Kramer JM, Stinchcomb D, Ambros V (1991) Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. EMBO J 10:3959-3970
24. Poinat P, De Arcangelis A, Sookhareea S, Zhu X, Hedgecock EM, Labouesse M, Georges-Labouesse E (2002) A conserved interaction between betal integrin/PAT-3 and Nek-interacting kinase/MIG-15 that mediates commissural axon navigation in C. elegans. Curr Biol 12:622-631
25. Praitis V, Casey E, Collar D, Austin J (2001) Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157:1217-1226
26. Rand KN (1996) Crystal violet can be used to visualize DNA bands during gel electrophoresis and to improve cloning efficiency. Technical Tips Online 1:T40022
27. Sieburth D, Ch'ng Q, Dybbs M, Tavazoie M, Kennedy S, Wang D, Dupuy D, Ruai JF, Hill DE, Vidal M, Ruvkun G, Kaplan JM (2005) Systematic analysis of genes required for synapse structure and function. Nature 436:510-517
28. Simmer F, Tijsterman M, Parrish S, Koushika SP, Nonet ML, Fire A, Ahringer J, Plasterk RH (2002) Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr Biol 12:1317-1319
29. Smith NA, Singh SP, Wang MB, Stoutjesdijk PA, Green AG, Waterhouse PM (2000) Total silencing by intron-spliced hairpin RNAs. Nature 407:319-320
30. Sonnichsen B, Koski LB, Walsh A, Marschall P, Neumann B, Brehm M, Alleaume AM, Artelt J, Bettencourt P, Cassin E, Hewitson M, Holz C, Khan M, Lazik S, Martin C, Nitzsche B, Ruer M, Stamford J, Winzi M, Heinkel R, Roder M, Finell J, Hantsch H, Jones SJ, Jones M, Piano F, Gunsalus KC, Oegema K, Gonczy P, Coulson A, Hyman AA, Echeverri CJ (2005) Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans. Nature 434:462-469
31. Starr DA, Han M (2002) Role of ANC-1 in tethering nuclei to the actin cytoskeleton. Science 298:406-409
32. Stinchcomb DT, Shaw JE, Carr SH, Hirsh D (1985) Extrachromosomal DNA transformation of Caenorhabditis elegans. Mol Cell Biol 5:3484-3496
33. Tavernarakis N, Wang SL, Dorovkov M, Ryazanov A, Driscoll M (2000) Heritable and inducible genetic interference by double-stranded RNA encoded by transgenes. Nat Genet 24:180-183
34. Tijsterman M, May RC, Simmer F, Okihara KL, Plasterk RH (2004) Genes required for systemic RNA interference in Caenorhabditis elegans. Curr Biol 14:111-116
35. Timmons L (2004) Endogenous inhibitors of RNA interference in Caenorhabditis elegans. Bioessays 26:715-718
36. Timmons L, Court DL, Fire A (2001) Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene 263:103-112
37. Timmons L, Tabara H, Mello CC, Fire AZ (2003) Inducible systemic RNA silencing in Caenorhabditis elegans. Mol Biol Cell 14:2972-2983
38. Wang D, Kennedy S, Conte D Jr, Kim JK, Gabel HW, Kamath RS, Mello CC, Ruvkun G (2005) Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants. Nature 436:593-597
39. Waterston RH, Thomson JN, Brenner S (1980) Mutants with altered muscle structure of Caenorhabditis elegans. Dev Biol 77:271-302
40. Winston WM, Molodowitch C, Hunter CP (2002) Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1. Science 295:2456-2459