Differential effect of the low-molecular-weight heparin, dalteparin, and unfractionated heparin on microvascular endothelial cell hemostatic properties

Haematologica

Volumn 91, Issue 2, 2006, Pages 207-214

  Vignoli, Alfonso ^a   Marchetti, Marina ^a   Balducci, Donatella ^a   Barbui, Tiziano ^a   Falanga, Anna ^a  

^a OSPEDALI RIUNITI   (Italy)

Author keywords

Endothelial cells; Heparin; Low molecular weight heparin; Thrombomodulin; Tissue factor; Tissue factor pathway inhibitor

Indexed keywords

DALTEPARIN; HEPARIN; LIPOPOLYSACCHARIDE; LOW MOLECULAR WEIGHT HEPARIN; MESSENGER RNA; PROCOAGULANT; THROMBOPLASTIN; TISSUE FACTOR PATHWAY INHIBITOR;

ARTICLE; CELL STIMULATION; CONTROLLED STUDY; DRUG ACTIVITY; DRUG EFFECT; ENDOTHELIUM CELL; HEMOSTASIS; HUMAN; HUMAN CELL; PROTEIN EXPRESSION; STIMULUS RESPONSE; UMBILICAL VEIN; VASCULAR ENDOTHELIUM;

CELLS, CULTURED; DALTEPARIN; ENDOTHELIUM, VASCULAR; HEMOSTASIS; HEPARIN; HEPARIN, LOW-MOLECULAR-WEIGHT; HUMANS; MICROCIRCULATION;

EID: 33644544424     PISSN: 03906078     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Hirsh J, Warkentin TE, Shaughnessy SG, Anand SS, Halperin JL, Raschke R, et al. Heparin and low-molecular-weight heparin: mechanisms of action, pharmacokinetics, dosing, monitoring, efficacy, and safety. Chest 2001;119 Suppl 1:64S-94S.
2. Zacharski LR, Ornstein DL. Heparin and cancer. Thromb Haemost 1998;80: 10-23.
3. Elsayed E, Becker RC. The impact of heparin compounds on cellular inflammatory responses: a construct for future investigation and pharmaceutical development. J Thromb Thrombolysis 2003;15:11-8.
4. Cines DB, Pollak ES, Buck CA, Loscalzo J, Zimmerman GA, McEver RP, et al. Endothelial cells in physiology and in the pathophysiology of vascular disorders. Blood 1998;91:3527-61.
5. Esmon CT. Inflammation and thrombosis. J Thromb Haemost 2003;1:1343-8.
6. Perez-Ruiz A, Montes R, Carrasco P, Rocha E. Effects of a low molecular weight heparin, bemiparin, and unfractionated heparin on hemostatic properties of endothelium. Clin Appl Thromb Hemost 2002;8:65-71.
7. Martinez-Sales V, Vila V, Reganon E, Oms JG, Aznar J. Effect of unfractionated heparin and a low molecular weight heparin (enoxaparin) on coagulant activity of cultured human endothelial cells. Haematologica 2003; 88:694-9.
8. Lupu C, Poulsen E, Roquefeuil S, Westmuckett AD, Kakkar VV, Lupu F. Cellular effects of heparin on the production and release of tissue factor pathway inhibitor in human endothelial cells in culture. Arterioscler Thromb Vasc Biol 1999;19:2251-62.
9. Cadroy Y, Gaspin D, Dupouy D, Lormeau JC, Boneu B, Sie P. Heparin reverses the procoagulant properties of stimulated endothelial cells. Thromb Haemost 1996; 75:190-5.
10. Garlanda C, Dejana E. Heterogeneity of endothelial cells. Specific markers. Arterioscler Thromb Vasc Biol 1997; 17:1193-202.
11. Ribeiro MJ, Phillips DJ, Benson JM, Evatt BL, Ades EW, Hooper WC. Hemostatic properties of the SV-40 transfected human microvascular endothelial cell line (HMEC-1). A representative in vitro model for microvascular endothelium. Thromb Res 1995; 79:153-61.
12. Falanga A, Vignoli A, Marchetti M, Barbui T. Defibrotide reduces procoagulant activity and increases fibrinolytic properties of endothelial cells. Leukemia 2003;17:1636-42.
13. Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973;52:2745-56.
14. Falanga A, Marchetti M, Giovanelli S, Barbui T. All-trans-retinoic acid counteracts endothelial cell procoagulant activity induced by a human promyelocytic leukemia-derived cell line (NB4). Blood 1996;87:613-7.
15. Celi A, Pellegnni G, Lorenzet R, De Blasi A, Ready N, Furie BC, et al. P-selectin induces the expression of tissue factor on monocytes. Proc Natl Acad Sci USA 1994;91:8767-71.
16. Marchetti M, Vignoli A, Bani MR, Balducci D, Barbui T, Falanga A. All-trans retinoic acid modulates microvascular endothelial cell hemostatic properties. Haematologica 2003;88:895-905.
17. Tyrrell DJ, Horne AP, Holme KR, Preuss JM, Page CP. Heparin in inflammation: potential therapeutic applications beyond anticoagulation. Adv Pharmacol 1999;46:151-208.
18. Bajaj MS, Birktoft JJ, Steer SA, Bajaj SP. Structure and biology of tissue factor pathway inhibitor. Thromb Haemost 2001;86:959-72.
19. Sandset PM, Abildgaard U, Larsen ML. Heparin induces release of extrinsic coagulation pathway inhibitor (EPI). Thromb Res 1988;50:803-13.
20. Li Y, Rodriquez M, Spencer FA, Becker RC. Comparative effects of unfractionated heparin and low molecular weight heparin on vascular endothelial cell tissue factor pathway inhibitor release: a model for assessing intrinsic thromboresistance. J Thromb Thrombolysis 2002;14:123-9.
21. Westmuckett AD, Kakkar VV, Hamuro T, Lupu F, Lupu C. Bemiparin and fluid flow modulate the expression, activity and release of tissue factor pathway inhibitor in human endothelial cells in vitro. Thromb Haemost 2001;86:1547-54.
22. Crossman DC, Carr DP, Tuddenham EG, Pearson JD, McVey JH. The regulation of tissue factor mRNA in human endothelial cells in response to endotoxin or phorbol ester. J Biol Chem 1990;265:9782-7.
23. Daum G, Hedin U, Wang Y, Wang T, Clowes AW. Diverse effects of heparin on mitogen-activated protein kinase-dependent signal transduction in vascular smooth muscle cells. Circ Res 1997; 81:17-23.
24. Liu Y, Pelekanakis K, Woolkalis MJ. Thrombin and tumour necrosis factor alpha stimulate tissue factor expression in human endothelial cells: regulation through c-Fos and c-Jun. J Biol Chem 2004;279:36142-7.
25. Sohn RH, Deming CB, Johns DC, Champion HC, Bian C, Gardner K, et al. Regulation of endothelial thrombomodulin expression by inflammatory cytokines is mediated by activation of nuclear factor-k B. Blood 2005;105: 3910-7.
26. Ameri A, Kuppuswamy MN, Basu S, Bajaj SP. Expression of tissue factor pathway inhibitor by cultured endothelial cells in response to inflammatory mediators. Blood 1992;79:3219-26.
27. Hansen JB, Svensson B, Olsen R, Ezban M, Osterud B, Paulssen RH. Heparin induces synthesis and secretion of tissue factor pathway inhibitor from endothelial cells in vitro. Thromb Haemost 2000;83:937-43.
28. Sato Y, Kataoka H, Asada Y, Marutsuka K, Kamikubo Y, Koono M, et al. Overexpression of tissue factor pathway inhibitor in aortic smooth muscle cells inhibits cell migration induced by tissue factor/factor VIIa complex. Thromb Res 1999;94:401-6.
29. Bombeli T, Mueller M, Haeberli A. Anticoagulant properties of the vascular endothelium. Thromb Haemost 1997;77:408-23.
30. Nan B, Lin P, Lumsden AB, Yao Q, Chen C. Effects of TNF-α and curcumin on the expression of thrombomodulin and endothelial protein C receptor in human endothelial cells. Thromb Res 2005;115:417-26.
31. Khorana AA, Sahni A, Altiand OD, Francis CW. Heparin inhibition of endothelial cell proliferation and organization is dependent on molecular weight. Arterioscler Thromb Vasc Biol 2003;23:2110-5.