Intron encoded sequences necessary for trans splicing in transiently transfected Brugia malayi

Molecular and Biochemical Parasitology

Volumn 137, Issue 1, 2004, Pages 181-184

  Higazi, Tarig B ^a   Unnasch, Thomas R ^a  

^a UNIVERSITY OF ALABAMA SCHOOL OF MEDICINE   (United States)

Author keywords

Filariasis; heat shock protein 70; HSP70; nt; nucleotides; open reading frame; ORF; SL; spliced leader; Spliced leader; Splicing; Transfection

Indexed keywords

HEAT SHOCK PROTEIN;

ARTICLE; BRUGIA MALAYI; DNA SEQUENCE; GENE SEQUENCE; GENE STRUCTURE; GENETIC TRANSFECTION; INTRON; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANS SPLICING;

ANIMALS; BASE SEQUENCE; BRUGIA MALAYI; INTRONS; RNA, HELMINTH; RNA, MESSENGER; TRANS-SPLICING; TRANSFECTION;

BRUGIA; BRUGIA MALAYI; PROTOZOA;

EID: 3343007295     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2004.04.014     Document Type: Article

References (16)

1. Agami R., Aly R., Halman S., Shapira M. Functional analysis of cis-acting DNA elements required for expression of the SL RNA gene in the parasitic protozoan Leishmania amazonensis. Nucl. Acids Res. 22:1994;1959-1965
2. Henkle-Duhrsen K., Liebau E., Walter R.D. The Onchocerca volvulus Messenger RNAs for a HSP70, a collagen-like protein and a ribosomal protein possess a 5′ spliced leader sequence. Trop. Med. Parasitol. 44:1993;337-339
3. Davis R.E., Singh H., Botka C., Hardwick C., Elmeanawy M.A., Villanueva J. RNA trans-splicing in Fasciola hepatica - identification of a spliced leader (SL) RNA and SL sequences on mRNAs. J. Biol. Chem. 269:1994;20026-20030
4. Blaxter M., Liu L. Nematode spliced leaders - ubiquity, evolution and utility. Int. J. Parasitol. 26:1996;1025-1033
5. Donelson J.E., Zeng W. A comparison of trans-RNA splicing in trypanosomes and nematodes. Parasitol. Today. 6:1990;327-334
6. Maroney P.A., Denker J.A., Darzynkiewicz E., Laneve R., Nilsen T.W. Most mRNAs in the nematode Ascaris lumbricoides are trans-spliced: a role for spliced leader addition in translational efficiency. RNA. 1:1995;714-723
7. Blumenthal T. Trans-splicing and polycistronic transcription in Caenorhabditis elegans. Trends Genet. 11:1995;132-136
8. Nilsen T.W. Trans-splicing of nematode premessenger RNA. Ann. Rev. Microbiol. 47:1993;413-440
9. Hannon G.J., Maroney P.A., Denker J.A., Nilsen T.W. Trans splicing of nematode pre-messenger RNA in vitro. Cell. 61:1990;1247-1255
10. Hannon G.E., Hannon G.J., Maroney P.A., Nilsen T.W. Transcription of a nematode U1 small nuclear RNA in vitro - 3′-end formation requires cis-acting elements within the coding sequence. J. Biol. Chem. 269:1994;12387-12390
11. Denker J.A., Maroney P.A., Yu Y.T., Kanost R.A., Nilsen T.W. Multiple requirements for nematode spliced leader RNP function in trans-splicing. RNA. 2:1996;746-755
12. Denker J.A., Zuckerman D.M., Maroney P.A., Nilsen T.W. New components of the spliced leader RNP required for nematode trans-splicing. Nature. 417:2002;667-670
13. Maroney P.A., Yu Y.T., Jankowska M., Nilsen T.W. Direct analysis of nematode cis- and trans-spliceosomes: a functional role for U5 snRNA in spliced leader addition trans-splicing and the identification of novel Sm snRNPs. RNA. 2:1996;735-745
14. Rothstein N., Rajan T.V. Characterization of an HSP70 gene from the human filarial parasite, Brugia malayi (Nematoda). Mol. Biochem. Parasitol. 49:1991;229-237
15. Shu L., Katholi C., Higazi T., Unnasch T.R. Analysis of the Brugia malayi HSP70 promoter using a homologous transient transfection system. Mol. Biochem. Parasitol. 128:2003;67-75
16. Romfo C.M., Maroney P.A., Wu S., Nilsen T.W. 3′ splice site recognition in nematode trans-splicing involves enhancer-dependent recruitment of U2 snRNP. RNA. 7:2001;785-792