In vitro pollen tube growth reveals the cytotoxic potential of the flavonols, quercetin and rutin

Alternatives to Laboratory Animals

Volumn 32, Issue 2, 2004, Pages 79-90

  Antognoni, Fabiana ^a   Ovidi, Elisa ^b   Taddei, Anna Rita ^b   Gambellini, Gabriella ^b   Speranza, Anna ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF TUSCIA   (Italy)

Author keywords

Cytotoxicity; Pollen tube growth; Quercetin; Rutin

Indexed keywords

ANTIOXIDANT; FLAVONOL; GLYCOSIDE; QUERCETIN; RUTOSIDE;

ARTICLE; BIOSAFETY; CELL NUCLEUS; CELL ORGANELLE; CELL VACUOLE; CONGENITAL MALFORMATION; CYTOTOXICITY; DIAGNOSTIC TEST; DIET SUPPLEMENTATION; DNA DAMAGE; EXPERIMENTAL ANIMAL; FOOD INTAKE; GENOTOXICITY; IN VITRO STUDY; KIWIFRUIT; NONHUMAN; NUTRIENT; NUTRITION; PHOTOMETRY; PHYTOCHEMISTRY; POLLEN; POLLEN TUBE; POLLEN TUBE GROWTH TEST; PREVENTIVE MEDICINE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; SENSITIVITY AND SPECIFICITY; SUSPENSION CELL CULTURE; TOXICOLOGY;

ACTINIDIA DELICIOSA; ANIMALIA;

EID: 3343002113     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290403200205     Document Type: Article

References (34)

1. Peterson, J. & Dwyer, J. (1998). Flavonoids: dietary occurrence and biochemical activity. Nutrition Research 18, 1995-2018.
2. Beecher, G.R. (2003). Overview of dietary flavonoids: nomenclature, occurrence and intake. Journal of Nutrition 133, Suppl., 3248-3254.
3. Crespi, V., Morand, C., Besson, C., Manach, C., Demigne, C. & Remesy, C. (2002). Quercetin, but not its glycosides, is absorbed from the rat stomach. Journal of Agricultural Food Chemistry 50, 618-621.
4. Graefe, E.U., Wittig, J., Mueller, S., Riethling, A.K., Uehleke, B., Drewlow, B., Pforte, H., Jacobasch, G., Derendorf, H. & Veit, M. (2001). Pharmacokinetics and bioavailability of quercetin glycosides in humans. Journal of Clinical Pharmacology 41, 492-499.
5. Hertog, M.G.L., Kromhout, D., Aravanis, C., Blackburn, H., Buzina, R., Fidanza, F., Giampaoli, S., Jansen, A., Menotti, A., Nedeljkovic, S., Pekkarinen, M., Simic, B.S., Toshima, H., Feskens, E.J.M., Hollman, P.C.H. & Katan, M.B. (1995). Flavonoid intake and long-term risk of coronary heart disease and cancer in the seven countries study. Archives of International Medicine 155, 381-386.
6. Ren, W., Qiao, Z., Wang, H., Zhu, L. & Zhang, L. (2003). Flavonoids: promising anticancer agents. Medical Research Reviews 23, 519-534.
7. Zao, C., Shi, Y., Lin, W., Wang, W., Jia, Z., Yao, S., Fan, B. & Zheng, R. (2001). Fast repair of the radical cations of dCMP and poly C by quercetin and rutin. Mutagenesis 16, 271-275.
8. Gaspar, J., Rodrigues, A., Laires, A., Silva, F., Costa, S., Monteiro, M.J. & Rueff, J. (1994). On the mechanism of genotoxicity and metabolism of quercetin. Mutagenesis 9, 445-449.
9. Yamashita, N., Tanemura, H. & Kawanishi, S. (1999). Mechanism of oxidative DNA damage induced by quercetin in the presence of Cu(II). Mutation Research 425, 107-115.
10. Van der Hoeven, J.C.M., Bruggeman, I.M. & Debets, F.M.H. (1984). Genotoxicity of quercetin in cultured mammalian cells. Mutation Research 136, 9-21.
11. Kuhlmann, M.K., Horsch, E., Burkhardt, G., Wagner, M. & Kölher, H. (1998). Reduction of cisplatin toxicity in cultured renal tubular cells by the bioflavonoid quercetin. Archives of Toxicology 72, 536-540.
12. Bear, W.L. & Teel, R.W. (2000). Effects of citrus flavonoids on the mutagenicity of heterocyclic amines and on cytochrome P450 1A2 activity. Anticancer Research 20, 3609-3614.
13. Autore, G., Rastrelli, L., Lauro, M.R., Marzocco, S., Sorrentino, R., Sorrentino, U., Pinto, A. & Aquino, R. (2001). Inhibition of nitric oxide synthase expression by a methanolic extract of Crescentia alata and its derivative flavonols. Life Sciences 70, 523-534.
14. Taylor, L.P. & Hepler, P.K. (1977). Pollen germination and tube growth. Annual Review of Plant Physiology and Plant Molecular Biology 48, 461-491.
15. Kristen, U. (1996). Main features of basal cytotoxicity: sites of toxic action and interaction in the pollen tube cell. ATLA 24, 429-434.
16. Kristen, U. & Kappler, R. (1995). The pollen tube growth test. In Methods in Molecular Biology, Vol. 43, In Vitro Toxicity Testing Protocols (ed. S. O'Hare & C.K. Atterwill), pp. 189-198. Totowa, NJ, USA: Humana Press.
17. Kristen, U. (1997). Use of higher plants as screen for toxicity assessment. Toxicology in Vitro 11, 181-191.
18. Strube, K., Janke, D., Kappler, R. & Kristen, U. (1991). Toxicity of some herbicides to in vitro growing tobacco pollen tubes (the pollen test). Environmental and Experimental Botany 31, 217-222.
19. Gettings, S.D., Teal, J.J., Bagley, D.M., Demetrulias, J.L., Di Pasquale, L.C., Hintze, K.L., Rozen, M.G., Weise, S.L., Chudkowski, M., Marenus, K.D., Pape, W.J.W., Roddy, M.T., Schnetzinger, R., Silber, P.M., Glaza, S.M. & Kurtz, P.J. (1991). The CTFA Evaluation of Alternatives Program: an evaluation of in vitro alternatives to the Draize primary eye irritation test. (Phase I) hydro-alcoholic formulations; (Part 2) data analysis and biological significance. In Vitro Toxicology 4, 247-288.
20. Kristen, U., Hoppe, U. & Pape, W. (1993). The pollen tube growth test: a new alternative to the Draize eye irritation assay. Journal of the Society of Cosmetic Chemists 44, 153-162.
21. Gettings, S.D., Di Pasquale, L.C., Bagley, D.M., Casterton, P.L., Chudkowski, M., Curren, R.D., Demetrulias, J.L., Feder, P.I., Galli, C.L., Gay, R., Glaza, S.M., Hintze, K.L., Janus, J., Kurtz, P.J., Lordo, R.A., Marenus, K.D., Moral, J., Muscatiello, M., Pape, W.J.W., Renskers, K.J., Roddy, M.T. & Rozen, M.G. (1994). The CTFA Evaluation of Alternatives Program: an evaluation of in vitro alternatives to the Draize primary eye irritation test. (Phase II) oil/water emulsions. Food and Chemical Toxicology 32, 943-976.
22. Barile, F.A., Dierickx, P.J. & Kristen, U. (1994). In vitro cytotoxicity testing for prediction of acute human toxicity. Cell Biology and Toxicology 10, 155-162.
23. Clemedson, C., McFarlane-Abdulla, E., Andersson, M., Barile, F.A., Calleja, M.C., Chesné, C., Clothier, R., Cottin, M., Curren, R., Dierickx, P., Ferro, M., Fiskesjö, G., Garza-Ocafias, L., Gómez-Lechón, M.J., Gülden, M., Isomaa, B., Janus, J., Judge, P., Kahru, A., Kemp, R.B., Kerszman, G., Kristen, U., Kunimoto, M., Kärenlampi, S., Lavrijsen, K., Lewan, L., Lilius, H., Malmsten, A., Ohno, T., Persoone, G., Pettersson, R., Roguet, R., Romert, L., Sandberg, M., Sawyer, T.W., Seibert, H., Shrivastava, R., Sjoström, M., Stammati, A., Tanaka, N., Torres-Alanis, O., Voss, J-U., Wakuri, S., Walum, E., Wang, X., Zucco, F. & Ekwall, B. (1996). MEIC evaluation of acute systemic toxicity. Part II. In vitro results from 68 toxicity assays used to test the first 30 reference chemicals and a comparative cytotoxicity analysis. ATLA 24, Suppl. 1, 273-311.
24. Ekwall, B., Clemedson, C., Ekwall, B., Ring, P. & Romert, L. (1999). EDIT: a new international multicentre programme to develop and evaluate batteries of in vitro tests for acute and chronic systemic toxicity. ATLA 27, 339-349.
25. Speranza, A., Scoccianti, V., Crinelli, R., Calzoni, G.L. & Magnani, M. (2001). Inhibition of proteasome activity strongly affects kiwifruit pollen germination. Involvement of the ubiquitin/proteasome pathway as a major regulator. Plant Physiology 126, 1150-1161.
26. Calzoni, G.L., Borghini, F., Del Giudice, E., Betti, L., Dal Rio, F., Migliori, M., Trebbi, G. & Speranza, A. (2003). Weak extremely high frequency microwaves affect pollen-tube emergence and growth in kiwifruit: pollen grain irradiation and water-mediated effects. Journal of Alternative and Complementary Medicine 9, 217-233.
27. Scoccianti, V., Ovidi, E., Taddei, A.R., Tiezzi, A., Crinelli, R., Gentilini, L. & Speranza, A. (2003). Involvement of the ubiquitin/proteasome pathway in the organisation and polarised growth of kiwifruit pollen tubes. Sexual Plant Reproduction 16, 123-133.
28. Aherne, S.A. & O'Brien, N.M. (2000). Mechanism of protection by the flavonoids, quercetin and rutin, against tert-butylhydroperoxide- and menadione-induced DNA single strand breaks in Caco-2 cells. Free Radical Biology and Medicine 29, 507-514.
29. Fiorani, M., De Sanctis, R., De Bellis, R. & Dacha, M. (2002). Intracellular flavonoids as electron donors for extracellular ferricyanide reduction in human erythrocytes. Free Radical Biology and Medicine 32, 64-72.
30. Röhrdanz, E., Bittner, A., Tran-Thi, Q.H. & Kahl, R. (2003). The effect of quercetin on the mRNA expression of different antioxidant enzymes in rat hepatoma cells. Archives of Toxicology 77, 506-510.
31. Arora, A., Byrem, T.M., Nair, M.G. & Strasburg, G.M. (2000). Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Archives of Biochemistry and Biophysics 373, 102-109.
32. Saija, A., Scalese, M., Lanza, M., Marzullo, D., Bonina, F. & Castelli, F. (1995). Flavonoids as antioxidant agents: importance of their interaction with biomembranes. Free Radical Biology and Medicine 19, 481-486.
33. Kristen, U., Bischoff, N., Lisboa, S., Schirmer, E., Witt, S. & Quader, H. (2002). Morphology of cell injury: an approach to the EDIT programme by the use of tobacco pollen tubes. ATLA 30, 323-329.
34. Åstrom, H., Sorri, O. & Raudaskoski, M. (1995). Role of microtubules in the movement of the vegetative nucleus and generative cell in tobacco pollen tubes. Sexual Plant Reproduction 8, 61-69.