Expression and processing of hepatitis C virus structural proteins in Pichia pastoris yeast

Biochemical and Biophysical Research Communications

Volumn 342, Issue 2, 2006, Pages 625-631

  Martinez Donato, Gillian ^a   Acosta Rivero, Nelson ^a   Morales Grillo, Juan ^a   Musacchio, Alexis ^a   Vina, Ariel ^a   Alvarez, Catalina ^a   Figueroa, Nelvis ^a   Guerra, Ivis ^a   Garcia, Jose ^a   Varas, Laura ^a   Muzio, Verena ^a   Dueñas Carrera, Santiago ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Core; E1; E2; Hepatitis C; Pichia pastoris

Indexed keywords

POLYPROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CULTURE MEDIUM; HEPATITIS C VIRUS; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN STRUCTURE; YEAST;

ANTIGENS, VIRAL; CLONING, MOLECULAR; HEPACIVIRUS; HEPATITIS C ANTIGENS; PICHIA; PROTEIN PROCESSING, POST-TRANSLATIONAL; VIRAL CORE PROTEINS; VIRAL ENVELOPE PROTEINS; VIRAL STRUCTURAL PROTEINS;

HEPATITIS C VIRUS; PICHIA PASTORIS;

EID: 33344465872     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2006.01.157     Document Type: Article

References (33)

1. Q.L. Choo, G. Kuo, A.J. Weiner, L.R. Overby, D.W. Bradley, and M. Houghton Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome Science 244 1989 359 362
2. A. Grakoui, C. Wychowski, C. Lin, S.M. Feinstone, and C.M. Rice Expression and identification of hepatitis C virus polyprotein cleavage products J. Virol. 67 1993 1385 1395
3. M. Hijikata, N. Kato, Y. Ootsuyama, M. Nakagawa, and K. Shimotohno Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis Proc. Natl. Acad. Sci. USA 88 1991 5547 5551
4. N. Acosta-Rivero, A. Musacchio, L. Lorenzo, C. Alvarez, and J. Morales Processing of the Hepatitis C virus precursor protein expressed in the methylotrophic yeast Pichia pastoris Biochem. Biophys. Res. Commun. 295 2002 81 84
5. R. Bartenschlager In vitro models for hepatitis C Virus Res. 82 2002 25 32
6. E. Blanchard, D. Brand, S. Trassard, A. Goudeau, and P. Roingeard Hepatitis C virus-like particle morphogenesis J. Virol. 76 2002 4073 4079
7. E. Santolini, G. Migliaccio, and N. La Monica Biosynthesis and biochemical properties of the hepatitis C virus core protein J. Virol. 68 1994 3631 3641
8. P. Hussy, H. Langen, J. Mous, and H. Jacobsen Hepatitis C virus core protein: carboxy-terminal boundaries of two processed species suggest cleavage by a signal peptide peptidase Virology 224 1996 93 104
9. J. McLauchlan, M.K. Lemberg, G. Hope, and B. Martoglio Intramembrane proteolysis promotes trafficking of hepatitis C virus core protein to lipid droplets EMBO J. 21 2002 3980 3988
10. N. Acosta-Rivero, V. Falcon, C. Alvarez, A. Musacchio, G. Chinea, d.l.R. Cristina, A. Rodriguez, S. Duenas-Carrera, V. Tsutsumi, M. Shibayama, I. Menendez, J. Luna-Munoz, M.M. Miranda-Sanchez, J. Kouri, and J. Morales-Grillo Structured HCV nucleocapsids composed of P21 core protein assemble primary in the nucleus of Pichia pastoris yeast Biochem. Biophys. Res. Commun. 310 2003 48 53
11. T. Isoyama, S. Kuge, and A. Nomoto The core protein of hepatitis C virus is imported into the nucleus by transport receptor Kap123p but inhibits Kap121p-dependent nuclear import of yeast AP1-like transcription factor in yeast cells J. Biol. Chem. 277 2002 39634 39641
12. N. Majeau, V. Gagne, A. Boivin, M. Bolduc, J.A. Majeau, D. Ouellet, and D. Leclerc The N-terminal half of the core protein of hepatitis C virus is sufficient for nucleocapsid formation J. Gen. Virol. 85 2004 971 981
13. J. Dubuisson Folding, assembly and subcellular localization of hepatitis C virus glycoproteins Curr. Top. Microbiol. Immunol. 242 2000 135 148
14. Y. Matsuura, T. Suzuki, R. Suzuki, M. Sato, H. Aizaki, I. Saito, and T. Miyamura Processing of E1 and E2 glycoproteins of hepatitis C virus expressed in mammalian and insect cells Virology 205 1994 141 150
15. M. Houghton Strategies and prospects for vaccination against the hepatitis C viruses Curr. Top. Microbiol. Immunol. 242 2000 327 339
16. T.J. Liang, B. Rehermann, L.B. Seeff, and J.H. Hoofnagle Pathogenesis, natural history, treatment, and prevention of hepatitis C Ann. Intern. Med. 132 2000 296 305
17. N. Acosta-Rivero, J.C. Alvarez-Obregon, A. Musacchio, V. Falcon, S. Duenas-Carrera, J. Marante, I. Menendez, and J. Morales In vitro self-assembled HCV core virus-like particles induce a strong antibody immune response in sheep Biochem. Biophys. Res. Commun. 290 2002 300 304
18. G. Martínez-Donato, Y. Capdesuñer, N. Acosta-Rivero, A. Rodríguez, J. Morales-Grillo, E. Martínez, M. González, J.C. Alvarez-Obregon, S. Dueñas-Carrera, Multimeric HCV E2 protein obtained from Pichia pastoris cells induces a strong immune response in mice, Am. J. Biochem. 1 2005 (accepted for publication).
19. V. Falcon, C. Garcia, M.C. de la Rosa, I. Menendez, J. Seoane, and J.M. Grillo Ultrastructural and immunocytochemical evidences of core-particle formation in the methylotrophic Pichia pastoris yeast when expressing HCV structural proteins (core-E1) Tissue Cell 31 1999 117 125
20. N. Majeau, V. Gagne, M. Bolduc, and D. Leclerc Signal peptide peptidase promotes the formation of hepatitis C virus non-enveloped particles and is captured on the viral membrane during assembly J. Gen. Virol. 86 2005 3055 3064
21. V. Falcon, N. Acosta-Rivero, G. Chinea, J. Gavilondo, M.C. dela Rosa, I. Menendez, S. Duenas-Carrera, A. Vina, W. Garcia, B. Gra, M. Noa, E. Reytor, M.T. Barcelo, F. Alvarez, and J. Morales-Grillo Ultrastructural evidences of HCV infection in hepatocytes of chronically HCV-infected patients Biochem. Biophys. Res. Commun. 305 2003 1085 1090
22. L.J. Lorenzo, O. Garcia, N. Acosta-Rivero, S. Duenas-Carrera, G. Martinez, J. Alvarez-Obregon, D. Pichardo, A. Ramos, I. Guerra, and J. Morales Expression and immunological evaluation of the Escherichia coli-derived hepatitis C virus envelope E1 protein Biotechnol. Appl. Biochem. 32 2000 137 143
23. S. Dueñas-Carrera, J. Morales, N. Acosta-Rivero, L.J. Lorenzo, C. García, T. Ramos, I. Guerra, and M. Peña Variable level expression of Hepatitis C virus core protein in a prokaryotic system. Analysis of the humoral response in rabbit Biotecnología Aplicada 16 1999 226 231
24. J. Morales, A. Viña, C. Garcia, N. Acosta-Rivero, S. Dueñas-Carrera, O. Garcia, I. Guerra, V. Falcon, Sequences derived from the genome of the hepatitis C virus, and use thereof, WO 98/25960, 1998.
25. J.C. Meunier, A. Fournillier, A. Choukhi, A. Cahour, L. Cocquerel, J. Dubuisson, and C. Wychowski Analysis of the glycosylation sites of hepatitis C virus (HCV) glycoprotein E1 and the influence of E1 glycans on the formation of the HCV glycoprotein complex J. Gen. Virol. 80 1999 887 896
26. T. Slater-Handshy, D.A. Droll, X. Fan, A.M. Di Bisceglie, and T.J. Chambers HCV E2 glycoprotein: mutagenesis of N-linked glycosylation sites and its effects on E2 expression and processing Virology 319 2004 36 48
27. M. Esumi, N. Hayashi, H. Takahashi, T. Shikata, M. Moriyama, Y. Arakawa, T. Eto, T. Nishihara, C. Nozaki, and K. Mizuno Immunoreactive core peptides of hepatitis C virus produced in Escherichia coli and in vitro DNA amplification-restricted transcription-translation system J. Virol. Methods 59 1996 91 98
28. S. Boulant, M. Becchi, F. Penin, and J.P. Lavergne Unusual multiple recoding events leading to alternative forms of hepatitis C virus core protein from genotype 1b J. Biol. Chem. 278 2003 45785 45792
29. J.A. Fournillier, A. Cahour, N. Escriou, M. Girad, and C. Wychowski Processing of the E1 glycoprotein of hepatitis C virus expressed in mammalian cells J. Gen. Virol. 77 Pt. 5 1996 1055 1064
30. T. Slater-Handshy, D.A. Droll, X. Fan, A.M. Di Bisceglie, and T.J. Chambers HCV E2 glycoprotein: mutagenesis of N-linked glycosylation sites and its effects on E2 expression and processing Virology 319 2004 36 48
31. L. Cocquerel, J.C. Meunier, A. Pillez, C. Wychowski, and J. Dubuisson A retention signal necessary and sufficient for endoplasmic reticulum localization maps to the transmembrane domain of hepatitis C virus glycoprotein E2 J. Virol. 72 2004 2183 2191
32. J. Patel, A.H. Patel, and J. McLauchlan The transmembrane domain of the hepatitis C virus E2 glycoprotein is required for correct folding of the E1 glycoprotein and native complex formation Virology 279 2001 58 68
33. L. Cocquerel, C. Wychowski, F. Minner, F. Penin, and J. Dubuisson Charged residues in the transmembrane domains of hepatitis C virus glycoproteins play a major role in the processing, subcellular localization, and assembly of these envelope proteins J. Virol. 74 2000 3623 3633