Ultrastructural and immunocytochemical evidences of core-particle formation in the methylotrophic Pichia pastoris yeast when expressing HCV structural proteins (core-E1)

Tissue and Cell

Volumn 31, Issue 2, 1999, Pages 117-125

  Falcon V ^a   Garcia C ^a   De la Rosa, M C ^a   Menendez I ^a   Seoane, J ^a   Grillo, J M ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Core protein; HCV; Pichia pastoris; Transmission electron microscopy; Virus like particles

Indexed keywords

CORE PROTEIN; STRUCTURAL PROTEIN; VIRUS ANTIGEN; VIRUS PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL VACUOLE; CLONE; CRYSTALLOID; CYTOARCHITECTURE; ENDOPLASMIC RETICULUM; EXPRESSION VECTOR; HEPATITIS C VIRUS; MEMBRANE COMPONENT; MORPHOGENESIS; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; TRANSMISSION ELECTRON MICROSCOPY; VIRUS ASSEMBLY; VIRUS NUCLEOCAPSID; VIRUS PARTICLE;

EID: 0033133933     PISSN: 00408166     EISSN: None     Source Type: Journal    
DOI: 10.1054/tice.1999.0032     Document Type: Article

References (34)

1. Cabral, G. A., Marciano-Cabral, F. and Frank, G. A. et al. 1978. Cellular and humoral immunity in guines pigs to major polypeptides derived from hepatitis B surface antigen. Journal of General Virology 38, 339-350.
2. Canales, M., Enríquez, A and Ramos, E. et al. 1997. Large-scale production in Pichia pastoris of the recombinant vaccine GAVAC against cattle tick. Vaccine, 15, 414-422.
3. Douma, A. C., Veenhuis, M., Wim de Koning, Melchior, E. and Harder, W. 1985. Dihydroxyacetone synthase is localized in the peroxisomal matrix of methanol-grown polymorpha. Arch. in Microbiology 143, 237-243.
4. García. C., Barro, M. and Viña, A. et al. 1994. Predominance of type 1b hepatitis C virus infection in Cuban carriers. Biotechnología Aplicada 12, 71.
5. Gleeson, M. A. and Sudbery, P. E. 1988. The methylotrophic yeast. Yeast 4, 1-15.
6. Grakoui, A., McCourt, D. W., Wychowski, C., Feinstone, S. M. and Rice, C. M. 1993a. A second hepatitis C virus-encoded proteinase. Proc. Nat. Acad. Sci. USA 90, 10583-10587.
7. Grakoui, A., McCourt, D. W., Wychowski, C., Feinstone, S. M. and Rice, C. M. 1993b. Characterization of hepatitis C virus-encoded serine proteinase: Determination of proteinase-dependent polyprotein cleavage sites. Journal of Virology 67, 2832-2843.
8. Grakoui, A., Wychowski, C., Lin, C., Feinstone, S. M. and Rice, C. M. 1993c. Expression and identification of hepatitis C virus polyprotein cleavage products. Journal of Virology 67, 1385-1395.
9. Hijikata, M., Kato, N., Ootsuyama, Y., Nakagawa, M. and Shimotohno, K. 1991. Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis. Proc. Nat. Acad. Sci. USA 88, 5547-5551.
10. Hijikata, M., Mizushima, H., and Akagi, T. et al. 1993a. Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. Journal of Virology 67, 4665-4675.
11. Hijikata, M., Mizushima, H., and Tanji, Y. et al. 1993b. Proteolytic processing and membrane association of putative nonstructural protein of hepatitis C virus. Proc. Nat. Acad. Sci. USA 90, 10773-10777.
12. Hijikata, M., Shimizu, Y. K., and Kato, H. et al. 1993c. Equilibrium centrifugation studies of hepatitis C virus: Evidences for circulating immune complexes. Journal of Virology 67, 1953-1958.
13. Janda, M. and Ahlquist, P. 1993. RNA-dependent replication, transcription, and persistence of Brome Mosaic virus RNA replicons in S. cerevisiae. Cell 72, 961-970.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the bacteriophage T4. Nature 227, 680-685.
15. Lo, S.-Y, Selby, M. J. and Ou J.-H. 1996. Interaction between hepatitic C virus core protein and E1 envelope protein. Journal of Virology 70, 5177-5182.
16. Martínez, E., García, C. and Grillo, J. M. 1993. Rapid transformation of non-Saccharomyces yeast by electroporation. Biotechnology Techniques 7, 12, 895-896.
17. Masashi, Y., Yoshinobu, M., Yoshikane, H., Keishin, S. and Hiroshi, M. 1994. Dynamics of hepatitis B virus core antigen in a transformed yeast cell: Analysis with an inducible system. Journal of Electron Microscopy 43, 386-396.
18. Matsumoto, M., Hwang, S. B., Jeng, K.-S., Zhu, N. and Lai, M. M. C. 1996. Homotypic interaction and multimerization of hepatitis C virus core protein. Virology 218, 43-51.
19. Mizuno, M., Yamada, G., Tanaka, T., Shimotohno, K., Takatani, M. and Tsuji, T. 1995. Virion-like structures in HeLa G cells transfected with the full-length sequence of the hepatitis C virus genome. Gastroenterology 109, 1933-1940.
20. Pentón, E., Muzio, V. and González-Griego, M. 1994. The hepatitis B virus (HBV) infection and its prevention by a recombinant-DNA viral surface antigen (rec-HBsAg) vaccine. Biotecnología Aplicada 11, 1-11.
21. Price, B. D., Rueckert, R. R. and Ahlquist, P. 1996. Complete replication of an animal virus and maintenance of expression vectors derived from it in Saccharomyces cerevisiae. Proc. Nat. Acad. Sci. USA 93, 9465-9470.
22. Quadt, R., Ishikawa, M., Janda, M. and Ahlquist, P. 1995. Formation of brome mosaic virus RNA-dependent RNA polymerase in yeast requires coexpression of viral proteins and viral RNA. Proc. Nat. Acad. Sci. USA 92, 4892-4896.
23. Sambrook, J., Fritsch, E. F., and Maniatis, T. 1989. In: Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
24. Santolini, R., Migliaccio, G. and La Monica, N. 1994. Biosynthesis and biochemical properties of the hepatitis C virus core protein. Journal of Virology 68, 3631-3641.
25. Shimizu, Y. K., Feinstone, S. M., Kohara, M., Purcell, R. H. and Yoshikura, H. 1996. Hepatitis C virus: Detection of intracellular virus particles by electron microscopy. Hepatology 23, 205-209.
26. Spurr, A. R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructural Research 26, 31-43.
27. Sugrue, R. J., Fu, J., Howe, J. and Chan, Y.-C. 1997. Expression of the dengue virus structural proteins in Pichia pastoris leads to the generation of virus-like particles. Journal of General Virology 78, 1861-1866.
28. Sung, J. S., Diwan, A. R., Falkler, W. A., Jr, Yang, H.-Y. and Halstead, S. B. 1975. Dengue carrier culture and antigen production in human lymphoblastoid lines. Intervirology 5, 137-149.
29. Takahashi, K., Kishimoto, S., Yoshizawa, H., Okamoto, H., Yoshikawa, A. and Mishiro, S. 1992. p26 protein and 33-nm particle associated with nucleocapsid of hepatitis C recovered from the circulation of infected hosts. Virology 191, 431-434.
30. Takamizawa, A., Mori, C., and Fuke, I. et al. 1991. Structure and organization of the hepatitis C virus genome isolated from human carriers. Journal of Virology 65, 1105-1113.
31. Van der Klei, I. J., Harder, W. and Veenhuis, M. 1991. Biosynthesis and assembly of alcohol oxidase, a peroxisomal matrix protein in methylotrophic yeast: A review. Yeast 7, 195-209.
32. Yong, V., Herrera, L., and Margolles, E. et al. 1991. Method for the Expression os Heterologous Genes in the Yeast Pichia Pastoris, Expression Vectors and Transformed Microorganisms. European Patent Application. Publication number: 0438 200 A1.
33. Yong, V., Gonzales, M.E., Herrera, L. and Delgado, J. 1992. El gen His3 complementa una mutación his de Pichia pastoris. Biotecnologia Aplicada 9, 55-61.
34. Yoshida, S., Manabe, S., and Tanishita, O. et al. 1996. Assembly of hepatitis C virus antigens expressed from a recombinant vaccinia virus. Proceeding of the Japan Academy 72, 73-78.